CERT1 (NM_001130105) Human Tagged ORF Clone

CAT#: RC226135

  • TrueORF®

COL4A3BP (Myc-DDK-tagged)-Human collagen, type IV, alpha 3 (Goodpasture antigen) binding protein (COL4A3BP), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001130105" in other vectors (4)

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CNY 7,890.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-COL4A3BP Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CERT; CERTL; COL4A3BP; GPBP; MRD34; STARD11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC226135 representing NM_001130105
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCACAGCTGCATCCCTACCCCGCCCTCTCCTTTCTCCGCTCCTCCTGCTTTTCTACCCGTCGTCA
CCCGGGAGAGCCGGAGGGGGCTAAGTTCGGGTGGCAGCGCCGGGCGCAACGCAGGGGTCACGGCGACGGC
GGCGGCGGCTGACGGCTGGAAGGGTAGGCTTCCTTCACCGCTCGTCCTCCTTCCTCGCTCCGCTCGGTGT
CAGGCGCGGCGGCGGCGCGGCGGGCGGACTTCGTCCCTCCTCCTGCTCCCCCCCACACCGGAGCGGGCAC
TCTTCGCTTCGCCATCCCCCGACCCTTCACCCCGAGGACTGGGCGCCTCCTCCGGCGCAGCTGAGGGAGC
GGGGGCCGGTCTCCTGCTCGGTTGTCGAGCCTCCATGTCGGATAATCAGAGCTGGAACTCGTCGGGCTCG
GAGGAGGATCCAGAGACGGAGTCTGGGCCGCCTGTGGAGCGCTGCGGGGTCCTCAGTAAGTGGACAAACT
ACATTCATGGGTGGCAGGATCGTTGGGTAGTTTTGAAAAATAATGCTCTGAGTTACTACAAATCTGAAGA
TGAAACAGAGTATGGCTGCAGAGGATCCATCTGTCTTAGCAAGGCTGTCATCACACCTCACGATTTTGAT
GAATGTCGATTTGATATTAGTGTAAATGATAGTGTTTGGTATCTTCGTGCTCAGGATCCAGATCATAGAC
AGCAATGGATAGATGCCATTGAACAGCACAAGACTGAATCTGGATATGGATCTGAATCCAGCTTGCGTCG
ACATGGCTCAATGGTGTCCCTGGTGTCTGGAGCAAGTGGCTACTCTGCAACATCCACCTCTTCATTCAAG
AAAGGCCACAGTTTACGTGAGAAGTTGGCTGAAATGGAAACATTTAGAGACATCTTATGTAGACAAGTTG
ACACGCTACAGAAGTACTTTGATGCCTGTGCTGATGCTGTCTCTAAGGATGAACTTCAAAGGGATAAAGT
GGTAGAAGATGATGAAGATGACTTTCCTACAACGCGTTCTGATGGTGACTTCTTGCATAGTACCAACGGC
AATAAAGAAAAGTTATTTCCACATGTGACACCAAAAGGAATTAATGGTATAGACTTTAAAGGGGAAGCGA
TAACTTTTAAAGCAACTACTGCTGGAATCCTTGCAACACTTTCTCATTGTATTGAACTAATGGTTAAACG
TGAGGACAGCTGGCAGAAGAGACTGGATAAGGAAACTGAGAAGAAAAGAAGAACAGAGGAAGCATATAAA
AATGCAATGACAGAACTTAAGAAAAAATCCCACTTTGGAGGACCAGATTATGAAGAAGGCCCTAACAGTC
TGATTAATGAAGAAGAGTTCTTTGATGCTGTTGAAGCTGCTCTTGACAGACAAGATAAAATAGAAGAACA
GTCACAGAGTGAAAAGGTGAGATTACATTGGCCTACATCCTTGCCCTCTGGAGATGCCTTTTCTTCTGTG
GGGACACATAGATTTGTCCAAAAGCCCTATAGTCGCTCTTCCTCCATGTCTTCCATTGATCTAGTCAGTG
CCTCTGATGATGTTCACAGATTCAGCTCCCAGGTTGAAGAGATGGTGCAGAACCACATGACTTACTCATT
ACAGGATGTAGGCGGAGATGCCAATTGGCAGTTGGTTGTAGAAGAAGGAGAAATGAAGGTATACAGAAGA
GAAGTAGAAGAAAATGGGATTGTTCTGGATCCTTTAAAAGCTACCCATGCAGTTAAAGGCGTCACAGGAC
ATGAAGTCTGCAATTATTTCTGGAATGTTGACGTTCGCAATGACTGGGAAACAACTATAGAAAACTTTCA
TGTGGTGGAAACATTAGCTGATAATGCAATCATCATTTATCAAACACACAAGAGGGTGTGGCCTGCTTCT
CAGCGAGACGTATTATATCTTTCTGTCATTCGAAAGATACCAGCCTTGACTGAAAATGACCCTGAAACTT
GGATAGTTTGTAATTTTTCTGTGGATCATGACAGTGCTCCTCTAAACAACCGATGTGTCCGTGCCAAAAT
AAATGTTGCTATGATTTGTCAAACCTTGGTAAGCCCACCAGAGGGAAACCAGGAAATTAGCAGGGACAAC
ATTCTATGCAAGATTACATATGTAGCTAATGTGAACCCTGGAGGATGGGCACCAGCCTCAGTGTTAAGGG
CAGTGGCAAAGCGAGAGTATCCTAAATTTCTAAAACGTTTTACTTCTTACGTCCAAGAAAAAACTGCAGG
AAAGCCTATTTTGTTC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC226135 representing NM_001130105
Red=Cloning site Green=Tags(s)

MQHSCIPTPPSPFSAPPAFLPVVTRESRRGLSSGGSAGRNAGVTATAAAADGWKGRLPSPLVLLPRSARC
QARRRRGGRTSSLLLLPPTPERALFASPSPDPSPRGLGASSGAAEGAGAGLLLGCRASMSDNQSWNSSGS
EEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNALSYYKSEDETEYGCRGSICLSKAVITPHDFD
ECRFDISVNDSVWYLRAQDPDHRQQWIDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFK
KGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHSTNG
NKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREDSWQKRLDKETEKKRRTEEAYK
NAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDAFSSV
GTHRFVQKPYSRSSSMSSIDLVSASDDVHRFSSQVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRR
EVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPAS
QRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDN
ILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001130105
ORF Size 2256 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001130105.1, NP_001123577.1
RefSeq ORF 2259 bp
Locus ID 10087
UniProt ID Q9Y5P4
MW 83.5 kDa
Gene Summary This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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