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NELF (NSMF) (NM_001130971) Human Tagged ORF Clone
CAT＃: RC225867
- TrueORF®
NSMF (Myc-DDK-tagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 4
ORF Plasmid: tGFP
"NM_001130971" in other vectors (4)
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CNY 5,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HH9; NELF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC225867 representing NM_001130971
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCGCCGCCTCCAGGAGGAGGGCGCTGAGGAGCGAGGCCATGTCCTCGGTGGCGGCCAAAGTGC GAGCAGCCCGAGCGTTTGGAGAGTACCTGTCCCAGAGTCACCCTGAGAACCGCAACGGCGCAGATCACCT GCTGGCTGATGCCTACTCTGGCCACGACGGGTCCCCCGAGATGCAGCCGGCCCCCCAGAACAAGCGCCGC CTGTCCCTCGTCTCCAACGGCTGCTACGAGGGCAGCCTCTCAGAGGAGCCCAGCATTAGGAAGCCCGCAG GCGAGGGCCCTCAGCCTCGAGTGTACACCATCTCTGGGGAGCCTGCCCTGCTGCCCAGCCCTGAGGCGGA GGCCATTGAGCTGGCGGTGGTGAAGGGGCGGCGGCAGCGGCACCCTCACCATCACAGCCAGCCCCTGCGC GCCAGCCCTGGTGGCAGCCGGGAGGACGTCAGCAGGCCCTGCCAGAGCTGGGCGGGCAGCCGCCAGGGCT CCAAGGAGTGCCCCGGATGTGCCCAGCTGGCTCCTGGCCCCACCCCTCGGGCCTTTGGGCTGGACCAGCC ACCTCTGCCTGAGACCTCCGGTCGCCGCAAGAAGCTGGAGAGGATGTACAGCGTTGACCGTGTGTCTGAC GACATCCCTATTCGTACCTGGTTCCCCAAGGAAAATCTTTTCAGCTTCCAGACAGCAACCACAACTATGC AAGCGAACTTCCGCAAACACCTGCGCATGGTCGGCAGCCGGAGGGTGAAGGCCCAGACGTTCGCTGAGCG GCGCGAGCGGAGCTTCAGCCGGTCCTGGAGCGACCCCACCCCCATGAAAGCCGACACTTCCCACGACTCC CGAGACAGCAGTGACCTGCAGAGCTCCCACTGCACGCTGGACGAGGCCTTCGAGGACCTGGACTGGGACA CTGAGAAGGGCCTGGAGGCTGTGGCCTGCGACACCGAAGGCTTCGTGCCACCAAAGGTCATGCTCATTTC CTCCAAGGTGCCCAAGGCTGAGTACATCCCCACTATCATCCGCCGGGATGACCCCTCCATCATCCCCATC CTCTACGACCATGAGCACGCAACCTTCGAGGACATCCTTGAGGAGATAGAGAGGAAGCTGAACGTCTACC ACAAGGGAGCCAAGATCTGGAAAATGCTGATTTTCTGCCAGGGAGGTCCTGGACACCTCTATCTCCTCAA GAACAAGGTGGCCACCTTTGCCAAAGTGGAGAAGGAAGAGGACATGATTCACTTCTGGAAGCGGCTGAGC CGCCTGATGAGCAAAGTGAACCCAGAGCCGAACGTCATCCACATCATGGGCTGCTACATTCTGGGGAACC CCAATGGAGAGAAGCTGTTCCAGAACCTCAGGACCCTCATGACTCCTTATAGGGTCACCTTCGAGTCACC CCTGGAGCTCTCAGCCCAAGGGAAGCAGATGATCGAGACGTACTTTGACTTCCGGTTGTATCGCCTGTGG AAGAGCCGCCAGCACTCGAAGCTGCTGGACTTTGACGACGTCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225867 representing NM_001130971
Red=Cloning site Green=Tags(s) MGAAASRRRALRSEAMSSVAAKVRAARAFGEYLSQSHPENRNGADHLLADAYSGHDGSPEMQPAPQNKRR LSLVSNGCYEGSLSEEPSIRKPAGEGPQPRVYTISGEPALLPSPEAEAIELAVVKGRRQRHPHHHSQPLR ASPGGSREDVSRPCQSWAGSRQGSKECPGCAQLAPGPTPRAFGLDQPPLPETSGRRKKLERMYSVDRVSD DIPIRTWFPKENLFSFQTATTTMQANFRKHLRMVGSRRVKAQTFAERRERSFSRSWSDPTPMKADTSHDS RDSSDLQSSHCTLDEAFEDLDWDTEKGLEAVACDTEGFVPPKVMLISSKVPKAEYIPTIIRRDDPSIIPI LYDHEHATFEDILEEIERKLNVYHKGAKIWKMLIFCQGGPGHLYLLKNKVATFAKVEKEEDMIHFWKRLS RLMSKVNPEPNVIHIMGCYILGNPNGEKLFQNLRTLMTPYRVTFESPLELSAQGKQMIETYFDFRLYRLW KSRQHSKLLDFDDVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130971 |
| ORF Size | 1515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130971.1, NP_001124443.1 |
| RefSeq ORF | 1518 bp |
| Locus ID | 26012 |
| UniProt ID | Q6X4W1 |
| Protein Families | Druggable Genome |
| MW | 57 kDa |
| Gene Summary | The protein encoded by this gene is involved in guidance of olfactory axon projections and migration of luteinizing hormone-releasing hormone neurons. Defects in this gene are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC225867L3 | Lenti-ORF clone of NSMF (Myc-DDK-tagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 4 |
CNY 6,940.00 |
|
| RC225867L4 | Lenti-ORF clone of NSMF (mGFP-tagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 4 |
CNY 6,940.00 |
|
| RG225867 | NSMF (tGFP-tagged) - Human nasal embryonic LHRH factor (NELF), transcript variant 4 |
CNY 5,510.00 |
|
| SC325140 | NSMF (untagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 4 |
CNY 7,220.00 |
