Troponin T1 (TNNT1) (NM_001126132) Human Tagged ORF Clone
CAT#: RC225355
- TrueORF®
TNNT1 (Myc-DDK-tagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 2
ORF Plasmid: tGFP
"NM_001126132" in other vectors (4)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ANM; NEM5; STNT; TNT; TNTS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225355 representing NM_001126132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACACCGAGGAGCAGGAATATGAGGAGGAGCAGCCGGAAGAGGAGGCTGCGGAGGAGGAGGAGG AAGCCCCCGAAGAGCCGGAGCCGGTGGCAGAGCCAGAAGAGGAACGCCCCAAACCAAGCCGCCCCGTGGT GCCTCCTTTGATCCCGCCAAAGATCCCAGAAGGGGAGCGCGTTGACTTCGATGACATCCACCGCAAGCGC ATGGAGAAAGACCTGCTGGAGCTGCAGACACTCATCGATGTACATTTCGAGCAGCGGAAGAAGGAGGAAG AGGAGCTGGTTGCCTTGAAGGAGCGCATTGAGCGGCGCCGGTCAGAGAGAGCCGAGCAACAGCGCTTCAG AACTGAGAAGGAACGCGAACGTCAGGCTAAGCTGGCGGAGGAGAAGATGAGGAAGGAAGAGGAAGAGGCC AAGAAGCGGGCAGAGGATGATGCCAAGAAAAAGAAGGTGCTGTCCAACATGGGGGCCCATTTTGGCGGCT ACCTGGTCAAGGCAGAACAGAAGCGTGGTAAGCGGCAGACGGGGCGGGAGATGAAGGTGCGCATCCTCTC CGAGCGTAAGAAGCCTCTGGACATTGACTACATGGGGGAGGAACAGCTCCGGGAGAAAGCCCAGGAGCTG TCGGACTGGATCCACCAGCTGGAGTCTGAGAAGTTCGACCTGATGGCGAAGCTGAAACAGCAGAAATATG AGATCAACGTGCTGTACAACCGCATCAGCCACGCCCAGAAGTTCCGGAAGGGGGCAGGGAAGGGCCGCGT TGGAGGCCGCTGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225355 representing NM_001126132
Red=Cloning site Green=Tags(s) MSDTEEQEYEEEQPEEEAAEEEEEAPEEPEPVAEPEEERPKPSRPVVPPLIPPKIPEGERVDFDDIHRKR MEKDLLELQTLIDVHFEQRKKEEEELVALKERIERRRSERAEQQRFRTEKERERQAKLAEEKMRKEEEEA KKRAEDDAKKKKVLSNMGAHFGGYLVKAEQKRGKRQTGREMKVRILSERKKPLDIDYMGEEQLREKAQEL SDWIHQLESEKFDLMAKLKQQKYEINVLYNRISHAQKFRKGAGKGRVGGRWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001126132 |
ORF Size | 786 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001126132.3 |
RefSeq ORF | 789 bp |
Locus ID | 7138 |
UniProt ID | P13805 |
Protein Families | Druggable Genome |
MW | 31.1 kDa |
Gene Summary | This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC225355L3 | Lenti-ORF clone of TNNT1 (Myc-DDK-tagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 2 |
CNY 5,890.00 |
|
RC225355L4 | Lenti-ORF clone of TNNT1 (mGFP-tagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 2 |
CNY 5,890.00 |
|
RG225355 | TNNT1 (tGFP-tagged) - Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 2 |
CNY 4,370.00 |
|
SC322933 | TNNT1 (untagged)-Human troponin T type 1 (skeletal, slow) (TNNT1), transcript variant 2 |
CNY 3,990.00 |
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