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NRDE2 (NM_017970) Human Tagged ORF Clone
CAT＃: RC224847
- TrueORF®
NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1
ORF Plasmid: tGFP
"NM_017970" in other vectors (6)
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CNY 12,160.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C14orf102 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224847 representing NM_017970
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGTTCCCAGCCTTTGCGGGGCTTAGTGAGGCTCCCGATGGCGGGAGCTCCAGGAAAGAGTTAG ACTGGCTGAGCAACCCAAGCTTTTGTGTTGGATCCATAACGTCCCTGAGCCAACAAACTGAAGCAGCTCC AGCCCATGTTTCTGAAGGGTTACCGCTGACAAGGAGTCATCTGAAATCAGAGTCTTCAGATGAAAGTGAC ACTAACAAAAAGCTCAAACAAACAAGTAGAAAAAAGAAGAAAGAGAAAAAGAAAAAAAGGAAGCATCAGC ATCATAAGAAAACAAAGAGGAAGCATGGGCCGTCGAGTAGCAGCAGGTCTGAGACAGACACCGATTCTGA AAAGGACAAACCTTCCAGAGGCGTTGGAGGCAGTAAAAAGGAATCTGAGGAACCGAATCAAGGAAATAAT GCTGCAGCTGATACTGGACATCGCTTTGTTTGGCTTGAGGACATTCAGGCTGTGACGGGAGAAACCTTCA GAACAGATAAGAAACCAGATCCTGCGAACTGGGAGTACAAGTCTCTCTACCGAGGGGATATAGCAAGATA CAAGAGGAAAGGAGACTCCTGCCTTGGCATTAACCCTAAGAAGCAGTGCATATCTTGGGAAGGGACTTCC ACAGAGAAGAAGCATTCACGCAAGCAGGTTGAACGCTATTTTACTAAGAAGAGTGTGGGATTAATGAACA TCGATGGAGTTGCCATTAGCAGTAAAACTGAACCTCCCTCATCTGAGCCCATCTCCTTTATACCAGTGAA GGACTTGGAAGATGCGGCTCCTGTTACAACCTGGTTGAATCCTCTGGGGATTTATGATCAGTCAACCACA CATTGGCTACAAGGACAGGGTCCTCCAGAGCAGGAATCAAAGCAGCCAGACGCACAGCCAGACAGCGAGA GTGCGGCTCTCAAGGCCAAGGTGGAGGAGTTTAACAGGAGGGTGCGGGAGAATCCTCGGGATACGCAGCT GTGGATGGCATTTGTTGCTTTTCAGGACGAGGTCATGAAAAGTCCTGGCCTGTATGCCATCGAGGAAGGA GAGCAGGAAAAGCGAAAGAGGTCCCTGAAGCTCATTCTGGAGAAGAAGCTGGCCATTCTGGAGCGGGCCA TTGAGAGCAACCAGAGCAGTGTGGATCTGAAACTGGCCAAGCTGAAGCTCTGCACAGAGTTCTGGGAGCC CTCCACTCTGGTCAAAGAGTGGCAGAAACTGATATTTTTGCATCCCAATAATACAGCCCTTTGGCAGAAA TACCTTTTATTTTGCCAGAGCCAGTTTAGTACCTTTTCGATATCAAAAATTCACAGTCTTTATGGAAAAT GCTTGAGCACTTTGTCTGCTGTTAAGGACGGCAGCATCTTATCTCACCCTGCGTTGCCTGGCACGGAAGA GGCCATGTTTGCACTCTTTCTTCAGCAGTGCCACTTTCTGCGGCAGGCTGGCCACTCTGAGAAGGCCATC TCATTGTTCCAGGCCATGGTGGACTTCACCTTCTTCAAACCCGACAGCGTGAAAGATCTGCCTACCAAAG GACAGGTGGAATTCTTTGAACCCTTTTGGGACAGTGGAGAGCCCCGGGCTGGGGAGAAGGGAGCCCGAGG CTGGAAGGCGTGGATGCACCAGCAGGAACGAGGTGGCTGGGTGGTCATCAACCCAGATGAGGATGACGAT GAACCAGAAGAGGATGACCAGGAAATAAAAGATAAGACTCTGCCCAGGTGGCAGATCTGGCTTGCTGCTG AGCGTTCCCGTGACCAGAGGCACTGGCGGCCCTGGCGCCCTGATAAGACCAAGAAGCAAACCGAGGAAGA CTGTGAGGATCCCGAGAGACAGGTGTTGTTTGATGATATTGGGCAATCTTTGATCAGACTTTCCAGCCAT GATCTTCAGTTCCAGCTGGTGGAGGCCTTCCTGCAGTTCTTGGGTGTGCCTTCTGGCTTTACTCCTCCAG CCTCCTGTCTTTATCTGGCCATGGATGAGAACAGCATCTTTGATAATGGACTTTATGATGAAAAGCCCTT GACTTTTTTCAACCCTTTGTTTTCTGGGGCTAGCTGTGTTGGCCGCATGGATAGGTTGGGCTATCCTCGC TGGACCAGGGGTCAGAACCGAGAGGGCGAGGAGTTCATCCGCAATGTCTTCCACCTTGTCATGCCTTTAT TTTCAGGCAAAGAGAAGTCCCAGCTCTGCTTCTCCTGGTTACAGTATGAGATTGCAAAGGTCATTTGGTG CCTGCACACTAAAAACAAGAAGAGATTAAAGTCTCAAGGGAAGAACTGCAAAAAACTAGCCAAGAATCTC CTTAAGGAGCCAGAAAACTGCAACAACTTTTGCCTGTGGAAGCAGTATGCACATCTGGAGTGGTTGCTTG GCAACACGGAGGATGCCAGAAAAGTTTTTGACACAGCACTTGGCATGGCAGGAAGCAGAGAACTGAAAGA CTCTGACCTCTGTGAGCTCAGTCTGCTCTATGCTGAGCTGGAGGTGGAGCTGTCGCCAGAAGTGAGAAGG GCTGCCACAGCTCGAGCTGTTCACATATTAACCAAGCTGACTGAGAGCAGCCCCTATGGGCCCTACACTG GACAGGTGTTGGCTGTTCACATTTTGAAAGCGCGAAAGGCTTATGAGCACGCACTGCAGGACTGTTTGGG TGACAGCTGTGTCTCCAATCCAGCTCCCACCGATTCCTGTAGCCGCCTAATTAGCCTGGCTAAATGCTTC ATGCTCTTCCAGTATTTGACCATAGGGATTGATGCTGCTGTGCAGATATACGAACAGGTGTTTGCAAAAC TGAACAGTTCTGTTTTCCCAGAAGGCTCTGGCGAGGGGGACAGTGCCAGCTCCCAGAGTTGGACCAGTGT TCTCGAAGCCATCACACTGATGCACACGAGCCTGCTGAGATTCCACATGAAAGTGAGTGTTTACCCGCTG GCCCCTCTGCGAGAGGCACTCTCACAGGCTTTAAAGTTGTATCCAGGCAACCAGGTTCTTTGGAGGTCCT ATGTACAGATTCAGAATAAGTCCCACAGTGCCAGCAAAACCAGGAGATTTTTTGACACAATCACCAGGTC TGCCAAACCCTTGGAGCCTTGGTTGTTTGCAATTGAAGCTGAGAAACTGAGGAAGAGACTGGTGGAAACT GTCCAGAGGTTAGACGGTAGAGAGATCCACGCCACAATTCCTGAGACCGGCTTAATGCATCGGATCCAAG CCCTGTTTGAAAATGCCATGCGCAGCGACAGTGGCAGCCAGTGCCCCTTGCTGTGGAGGATGTATTTGAA CTTTCTGGTTTCCTTAGGAAATAAAGAAAGAAGCAAAGGTGTATTCTACAAAGCACTTCAGAATTGCCCT TGGGCAAAGGTGTTGTACCTGGACGCCGTGGAGTATTTCCCCGATGAGATGCAGGAGATCCTGGACCTGA TGACTGAGAAGGAGCTCCGGGTGCGCCTGCCGCTGGAGGAGCTGGAGCTGCTGCTGGAGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224847 representing NM_017970
Red=Cloning site Green=Tags(s) MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESD TNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNN AAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTS TEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTT HWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEG EQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQK YLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFALFLQQCHFLRQAGHSEKAI SLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDD EPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSH DLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPR WTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNL LKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRR AATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCF MLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPL APLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVET VQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCP WAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017970 |
| ORF Size | 3492 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017970.4 |
| RefSeq Size | 3824 bp |
| RefSeq ORF | 3495 bp |
| Locus ID | 55051 |
| UniProt ID | Q9H7Z3 |
| MW | 132.5 kDa |
| Gene Summary | Protein of the nuclear speckles that regulates RNA degradation and export from the nucleus through its interaction with MTREX an essential factor directing various RNAs to exosomal degradation (PubMed:30842217). Changes the conformation of MTREX, precluding its association with the nuclear exosome and interaction with proteins required for its function in RNA exosomal degradation (PubMed:30842217). Negatively regulates, for instance, the degradation of mRNAs and lncRNAs by inhibiting their MTREX-mediated recruitment to nuclear exosome (PubMed:30842217). By preventing the degradation of RNAs in the nucleus, it promotes their export to the cytoplasm (PubMed:30842217).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor
,null,
RNA
,PubMed ID 30538148
[NRDE2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224847L1 | Lenti-ORF clone of NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 |
CNY 14,560.00 |
|
| RC224847L2 | Lenti-ORF clone of NRDE2 (mGFP-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 |
CNY 14,560.00 |
|
| RC224847L3 | Lenti-ORF clone of NRDE2 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 |
CNY 7,600.00 |
|
| RC224847L4 | Lenti-ORF clone of NRDE2 (mGFP-tagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 |
CNY 7,600.00 |
|
| RG224847 | NRDE2 (tGFP-tagged) - Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 |
CNY 13,760.00 |
|
| SC304549 | NRDE2 (untagged)-Human chromosome 14 open reading frame 102 (C14orf102), transcript variant 1 |
CNY 12,976.00 |
