FBLIM1 (NM_001024215) Human Tagged ORF Clone
CAT#: RC224749
FBLIM1 (Myc-DDK-tagged)-Human filamin binding LIM protein 1 (FBLIM1), transcript variant 2
ORF Plasmid: tGFP
"NM_001024215" in other vectors (4)
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CNY 3,656.00
CNY 4,370.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CAL; FBLP-1; FBLP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224749 representing NM_001024215
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCAAAGCCTGAGAAGAGGGTGGCATCGTCTGTCTTTATCACCCTGGCACCCCCGCGCCGCGATG TGGCCGTGGCGGAGGAAGTGAGGCAGGCAGTTTGTGAGGCCCGGCGTGGCCGCCCCTGGGAGGCTCCTGC CCCCATGAAGACACCCGAGGCTGGCTTGGCGGGGAGGCCCAGCCCCTGGACAACCCCTGGCAGAGCTGCA GCCACAGTGCCGGCTGCACCTATGCAGCTCTTCAATGGAGGATGCCCACCCCCTCCTCCTGTCCTGGATG GTGAGGACGTGCTTCCTGACCTGGACCTCCTCCCACCCCCTCCACCGCCCCCTCCAGTGCTTCTGCCTTC TGAAGAGGAGGCTCCTGCTCCAATGGGGGCCTCACTCATTGCAGACTTAGAGCAGCTGCACCTGTCCCCG CCCCCGCCCCCACCACAGGCCCCAGCGGAGGGACCTTCAGTCCAGCCCGGTCCCCTCAGGCCCATGGAGG AAGAGCTGCCACCTCCCCCGGCAGAACCTGTTGAGAAAGGGGCATCCACAGACATCTGTGCCTTCTGCCA CAAGACCGTGTCCCCCCGAGAGCTGGCTGTGGAGGCCATGAAGAGGCAGTACCATGCCCAGTGCTTCACG TGCCGCACCTGCCGCCGCCAGCTGGCTGGGCAGAGCTTCTACCAGAAGGATGGGCGACCCCTCTGCGAAC CCTGCTACCAGGACACACTGGAGAGGTGCGGCAAGTGTGGCGAGGTGGTCCGGGACCACATCATCAGGGC CCTGGGCCAGGCCTTCCACCCCTCCTGCTTCACGTGTGTGACCTGCGCCCGGTGCATTGGGGATGAGAGC TTTGCCCTGGGCAGCCAGAACGAGGTGTACTGCCTGGACGACTTCTACAGGTACGAGAAGGGTTTGTGCA CTGGGTGGGGTGCAGGGACAGGGCGAGACCCAAGCAGGGTGAAGGAGCTGAGCTTGAGTCCTGGGTGCTG GGCCAGAGTTTCCTGTCTACTGGTTTATTATAAAGAATATTACAGGGCTGGGCTGGGCGCGGTGGCTCAC GCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACGAGGTCAGGAGATCGAGATCATCCTG GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224749 representing NM_001024215
Red=Cloning site Green=Tags(s) MASKPEKRVASSVFITLAPPRRDVAVAEEVRQAVCEARRGRPWEAPAPMKTPEAGLAGRPSPWTTPGRAA ATVPAAPMQLFNGGCPPPPPVLDGEDVLPDLDLLPPPPPPPPVLLPSEEEAPAPMGASLIADLEQLHLSP PPPPPQAPAEGPSVQPGPLRPMEEELPPPPAEPVEKGASTDICAFCHKTVSPRELAVEAMKRQYHAQCFT CRTCRRQLAGQSFYQKDGRPLCEPCYQDTLERCGKCGEVVRDHIIRALGQAFHPSCFTCVTCARCIGDES FALGSQNEVYCLDDFYRYEKGLCTGWGAGTGRDPSRVKELSLSPGCWARVSCLLVYYKEYYRAGLGAVAH ACNPSTLGGRGGWITRSGDRDHPG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001024215 |
ORF Size | 1122 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024215.1, NP_001019386.1 |
RefSeq Size | 1521 bp |
RefSeq ORF | 1125 bp |
Locus ID | 54751 |
UniProt ID | Q8WUP2 |
MW | 40.1 kDa |
Gene Summary | This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224749L3 | Lenti-ORF clone of FBLIM1 (Myc-DDK-tagged)-Human filamin binding LIM protein 1 (FBLIM1), transcript variant 2 |
CNY 5,890.00 |
|
RC224749L4 | Lenti-ORF clone of FBLIM1 (mGFP-tagged)-Human filamin binding LIM protein 1 (FBLIM1), transcript variant 2 |
CNY 5,890.00 |
|
RG224749 | FBLIM1 (tGFP-tagged) - Human filamin binding LIM protein 1 (FBLIM1), transcript variant 2 |
CNY 4,370.00 |
|
SC302236 | FBLIM1 (untagged)-Human filamin binding LIM protein 1 (FBLIM1), transcript variant 2 |
CNY 6,080.00 |