MPRIP (NM_201274) Human Tagged Lenti ORF Clone
CAT#: RC224291L4
- LentiORF®
Lenti-ORF clone of MPRIP (mGFP-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2
"NM_201274" in other vectors (4)
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CNY 12,160.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | M-RIP; MRIP; p116Rip; RHOIP3; RIP3 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC224291).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_201274 |
ORF Size | 3075 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201274.3 |
RefSeq Size | 11023 bp |
RefSeq ORF | 3078 bp |
Locus ID | 23164 |
UniProt ID | Q6WCQ1 |
Protein Families | Druggable Genome |
MW | 116.5 kDa |
Gene Summary | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224291 | MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 7,136.00 |
|
RC224291L3 | Lenti-ORF clone of MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 12,160.00 |
|
RG224291 | MPRIP (tGFP-tagged) - Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 11,310.00 |
|
SC308006 | MPRIP (untagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 19,860.00 |