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MPRIP (NM_201274) Human Untagged Clone
CAT＃: SC308006
MPRIP (untagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2
CNY 19,860.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | M-RIP; MRIP; p116Rip; RHOIP3; RIP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308006 representing NM_201274.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTCCAGGCCAACATCTTCAACAAGAGCAAGTGTCAG AACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTAT GGCGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGACAACCCAGTGCACCGGTCTCGGAAATGG CAGCGACGGTTCTTCATCCTTTACGAGCACGGCCTCTTGCGCTACGCCCTGGATGAGATGCCCACGACC CTTCCTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGGCCGCACGGGCCAG AAGTTCTCCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCGGAGACCAAGGAGATCGTC AGTGGGTGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCAGAATCAGAAGAAGAAACGGAAA GTGGAGCCCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTTACCAGCAGCAGCAGCAGCAGC AGCAGCAGCAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCACCAAGTCCACACTCTGGCAGGAAGAA ATGAGGACCAAGGACCAGCCAGATGGCAGCAGCCTGAGTCCAGCTCAGAGTCCCAGCCAGAGCCAGCCT CCTGCTGCCAGCTCCCTGCGGGAACCTGGGCTAGAGAGCAAAGAAGAGGAGAGCGCCATGAGTAGCGAC CGCATGGACTGTGGCCGCAAAGTCCGGGTGGAGAGCGGCTACTTCTCTCTGGAGAAGACCAAACAGGAC TTGAAGGCTGAAGAACAGCAGCTGCCCCCGCCGCTCTCCCCTCCCAGCCCCAGCACCCCCAACCACAGG AGGTCCCAGGTGATTGAAAAGTTTGAGGCCTTGGACATTGAGAAGGCAGAGCACATGGAGACCAATGCA GTGGGGCCCTCACCATCCAGCGACACACGCCAGGGCCGCAGCGAGAAGAGGGCGTTCCCTAGGAAGCGG GACTTCACCAATGAAGCCCCCCCAGCTCCTCTCCCAGACGCCTCGGCTTCCCCCCTGTCTCCACACCGA AGAGCCAAGTCACTGGACAGGAGGTCCACGGAGCCCTCCGTGACGCCCGACCTGCTGAATTTCAAGAAA GGCTGGCTGACTAAGCAGTATGAGGACGGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCGATCAAAGC CTGAGATACTACAGGGATTCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTTGTCCGCA TGTTACGATGTCACAGAGTATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAGGGCGAG TTTACCCTGTCGGCCATGACATCTGGGATTCGGCGGAACTGGATCCAGACCATCATGAAGCACGTGCAC CCGACCACTGCCCCGGATGTGACCAGCTCGTTGCCAGAGGAAAAAAACAAGAGCAGCTGCTCTTTTGAG ACCTGCCCGAGGCCTACTGAGAAGCAAGAGGCAGAGCTGGGGGAGCCGGACCCTGAGCAGAAGAGGAGC CGCGCACGGGAGCGGAGGCGAGAGGGCCGCTCCAAGACCTTTGACTGGGCTGAGTTCCGTCCCATCCAG CAGGCCCTGGCTCAGGAGCGGGTGGGCGGCGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGAG GCGGAGCCTGGGGAGCTGGAGCGGGAGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCGCTTCGGGATG CTCGACGCCACAGACGGGCCAGGCACTGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCAGGG CTGCCTATGAGCGACCTCAAAACGCATAACGTCCACGTGGAGATTGAGCAGCGGTGGCATCAGGTGGAG ACCACACCTCTCCGGGAAGAGAAGCAGGTGCCCATCGCCCCCGTCCACCTGTCTTCTGAAGATGGGGGT GACCGGCTCTCCACACACGAGCTGACCTCTCTGCTCGAGAAGGAGCTGGAGCAGAGCCAGAAGGAGGCC TCAGACCTTCTGGAGCAGAACCGGCTCCTGCAGGACCAGCTGAGGGTGGCCCTGGGCCGGGAGCAGAGC GCCCGTGAGGGCTACGTGCTGCAGGCCACGTGCGAGCGAGGGTTTGCAGCAATGGAAGAAACGCACCAG AAGAAGATTGAAGATCTCCAGAGGCAGCACCAGCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGC CTCCTAGCCGAGGAGACAGCGGCCACCATCTCAGCCATCGAAGCCATGAAGAACGCCCACCGGGAGGAA ATGGAGCGGGAGCTGGAGAAGAGCCAGCGGTCCCAGATCAGCAGCGTCAACTCGGATGTTGAGGCCCTG CGGCGCCAGTACCTGGAGGAGCTGCAGTCGGTGCAGCGGGAACTGGAGGTCCTCTCGGAGCAGTACTCG CAGAAGTGCCTGGAGAATGCCCATCTGGCCCAGGCGCTGGAGGCCGAGCGGCAGGCCCTGCGGCAGTGC CAGCGTGAGAACCAGGAGCTCAATGCCCACAACCAGGAGCTGAACAACCGCCTGGCTGCAGAGATCACA CGGTTGCGGACGCTGCTGACTGGGGACGGCGGTGGGGAGGCCACTGGGTCACCCCTTGCACAGGGCAAG GATGCCTATGAACTAGAGGTCTTATTGCGGGTAAAGGAATCGGAAATACAGTACCTGAAACAGGAGATT AGCTCCCTCAAGGATGAGCTGCAGACGGCACTGCGGGACAAGAAGTACGCAAGTGACAAGTACAAAGAC ATCTACACAGAGCTCAGCATCGCGAAGGCTAAGGCTGACTGTGACATCAGCAGGTTGAAGGAGCAGCTC AAGGCTGCAACGGAAGCACTGGGGGAGAAGTCCCCTGACAGTGCCACGGTGTCCGGATATGATATAATG AAATCTAAAAGCAACCCTGACTTCTTGAAGAAAGACAGATCCTGTGTCACCCGGCAACTCAGAAACATC AGGTCCAAGTCCGTAATTGAGCAGGTCTCGTGGGATACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_201274 |
| Insert Size | 3078 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201274.3 |
| RefSeq Size | 11023 bp |
| RefSeq ORF | 3078 bp |
| Locus ID | 23164 |
| UniProt ID | Q6WCQ1 |
| Protein Families | Druggable Genome |
| MW | 116.5 kDa |
| Gene Summary | Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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A Novel Marker for Purkinje Cells, KIAA0864 Protein. An Analysis Based on a Monoclonal Antibody HFB-16 in Developing Human Cerebellum
,Yasuhiro Nakamura, Munehiko Yamamoto, Eriko Oda, Yonehiro Kanemura, Eri Kodama, Atsuyo Yamamoto, Hideyuki Yamamoto, Kenji Miyado, Hirotaka James Okano, Ryouji Fukagawa, Koichi Higaki, Mami Yamasaki, and Hideyuki Okano,
J. Histochem. Cytochem. 2005 Apr;53(4):423-30.
[MPRIP]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224291 | MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 7,136.00 |
|
| RC224291L3 | Lenti-ORF clone of MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 12,160.00 |
|
| RC224291L4 | Lenti-ORF clone of MPRIP (mGFP-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 12,160.00 |
|
| RG224291 | MPRIP (tGFP-tagged) - Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 |
CNY 11,310.00 |
