TSC22D1 (NM_183422) Human Tagged ORF Clone
CAT#: RC224087
- TrueORF®
TSC22D1 (Myc-DDK-tagged)-Human TSC22 domain family, member 1 (TSC22D1), transcript variant 1
ORF Plasmid: tGFP
"NM_183422" in other vectors (4)
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CNY 10,740.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Ptg-2; TGFB1I4; TSC22 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224087 representing NM_183422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCAGCCGCCTGAGTCCACCGCCGCGGCCGCCGCCGCTGCAGACATTAGCGCTAGGAAGATGGCGC ACCCGGCAATGTTCCCTCGAAGGGGCAGCGGTAGTGGCAGCGCCTCTGCTCTCAATGCAGCAGGTACCGG CGTCGGTAGTAATGCCACATCTTCCGAGGATTTTCCGCCTCCGTCGCTGCTTCAGCCGCCGCCCCCTGCA GCATCTTCTACGTCGGGACCACAGCCTCCGCCTCCACAAAGCCTGAACCTCCTTTCGCAGGCTCAGCTGC AGGCACAGCCTCTTGCGCCAGGCGGAACTCAAATGAAAAAGAAAAGTGGCTTCCAGATAACTAGCGTTAC TCCTGCTCAGATCTCCGCTAGTATCAGCTCTAACAACAGTATAGCAGAGGACACTGAGAGCTATGATGAT CTGGATGAATCTCACACGGAAGATCTCTCTTCTTCGGAGATCCTTGATGTGTCACTTTCCAGGGCTACTG ACTTAGGGGAGCCCGAACGCAGCTCCTCAGAAGAGACCCTAAATAACTTCCAGGAAGCCGAGACACCTGG GGCAGTCTCTCCCAACCAGCCCCACCTTCCTCAGCCTCATTTGCCTCACCTTCCACAACAGAATGTTGTG ATCAATGGGAATGCTCATCCACACCACCTCCATCACCACCATCAGATTCATCATGGGCACCACCTCCAAC ATGGTCACCACCATCCATCTCATGTTGCTGTGGCCAGTGCATCCATTACTGGTGGGCCACCCTCAAGCCC AGTATCTAGAAAACTCTCTACAACTGGAAGCTCTGACAGTATCACACCAGTTGCACCAACTTCTGCTGTA TCATCCAGTGGTTCACCTGCATCTGTAATGACTAATATGCGTGCTCCAAGTACTACAGGTGGAATAGGTA TAAATTCTGTTACTGGCACTAGTACAGTAAATAATGTTAACATTACTGCTGTGGGTAGTTTTAATCCTAA TGTGACAAGCAGCATGCTTGGTAATGTTAATATAAGTACAAGCAATATTCCTAGTGCTGCTGGTGTGAGT GTTGGGCCTGGAGTTACCAGTGGTGTTAATGTGAATATCTTGAGTGGCATGGGCAATGGTACTATTTCTT CCTCTGCTGCTGTTAGCAGTGTTCCTAATGCAGCTGCAGGGATGACTGGGGGATCGGTTTCAAGTCAGCA GCAACAACCAACAGTTAACACTTCGAGGTTCAGAGTTGTGAAGTTAGATTCTAGTTCTGAGCCCTTTAAA AAAGGTAGATGGACTTGCACTGAGTTCTATGAAAAAGAAAATGCTGTACCTGCTACAGAAGGTGTGCTGA TAAATAAAGTGGTGGAGACTGTAAAGCAAAATCCGATAGAAGTGACTTCTGAAAGGGAGAGCACTAGTGG GAGTTCAGTGAGCAGTAGTGTCAGCACACTGAGTCACTATACAGAGAGTGTGGGAAGTGGAGAGATGGGA GCCCCTACTGTGGTGGTGCAGCAGCAGCAGCAGCAACAACAACAACAACAGCAACAACCAGCTCTCCAAG GTGTGACCCTCCAACAGATGGATTTTGGTAGCACTGGTCCACAGAGTATTCCAGCAGTTAGTATACCACA GAGTATTTCTCAGTCACAGATCTCACAAGTACAATTACAGTCTCAAGAACTGAGCTATCAGCAAAAGCAA GGTCTTCAGCCAGTACCTCTGCAAGCCACTATGAGTGCTGCAACTGGTATCCAGCCATCGCCTGTAAATG TGGTTGGTGTAACTTCAGCTTTAGGTCAGCAGCCTTCCATTTCCAGTTTGGCTCAACCCCAGCTACCATA TTCTCAGGCGGCTCCTCCAGTGCAAACTCCCCTTCCAGGGGCACCACCACCCCAACAGTTACAGTATGGA CAACAGCAACCAATGGTTTCTACACAGATGGCCCCAGGCCATGTCAAATCAGTGACTCAAAATCCTGCTT CAGAGTATGTACAACAGCAGCCAATTCTTCAAACAGCAATGTCCTCCGGACAGCCCAGTTCTGCAGGAGT AGGAGCAGGAACAACAGTGATTCCTGTGGCTCAGCCACAGGGTATCCAGCTGCCAGTGCAGCCCACAGCA GTCCCAGCACAACCTGCAGGGGCATCTGTCCAGCCTGTTGGCCAGGCTCCGGCAGCAGTGTCTGCTGTAC CTACTGGCAGTCAGATTGCAAATATTGGTCAGCAAGCAAACATACCTACTGCAGTGCAGCAGCCCTCTAC CCAGGTTCCACCTTCAGTTATTCAGCAGGGTGCTCCTCCATCTTCGCAAGTGGTTCCACCTGCTCAAACT GGGATTATTCATCAGGGAGTTCAAACTAGTGCTCCAAGCCTTCCTCAACAATTGGTTATTGCATCCCAAA GTTCCTTGTTAACTGTGCCTCCCCAGCCACAAGGAGTAGAACCAGTAGCTCAAGGAATTGTTTCACAGCA GTTGCCTGCAGTTAGTTCTTTGCCCTCTGCTAGTAGTATTTCTGTTACAAGTCAGGTTAGTTCAACTGGT CCTTCTGGAATGCCTTCTGCCCCAACAAACTTGGTTCCACCACAAAATATAGCACAAACCCCTGCTACCC AAAATGGTAATTTGGTTCAAAGTGTTAGTCAACCTCCCTTGATAGCAACTAATACAAATTTGCCTTTGGC ACAACAGATACCACTAAGTTCTACCCAGTTCTCCGCACAATCATTAGCTCAGGCAATTGGAAGCCAAATT GAAGATGCCAGGCGTGCAGCGGAGCCCTCCTTAGTTGGCTTACCTCAGACTATCAGTGGTGACAGTGGGG GAATGTCAGCAGTTTCAGATGGGAGTAGCAGCAGCCTAGCAGCCTCTGCTTCTCTTTTCCCGTTGAAGGT GCTACCGCTGACGACACCCCTGGTGGATGGCGAGGATGAGAGCTCCTCTGGTGCAAGTGTGGTAGCTATT GACAACAAAATCGAGCAAGCTATGGATCTAGTGAAAAGCCATTTGATGTATGCGGTCAGAGAAGAAGTGG AGGTCCTCAAAGAGCAAATCAAAGAACTAATAGAGAAAAATTCCCAGCTGGAGCAGGAGAACAATCTGCT GAAGACACTGGCCAGTCCTGAGCAGCTTGCCCAGTTTCAGGCCCAGCTGCAGACTGGCTCCCCCCCTGCC ACCACCCAGCCACAGGGCACCACACAGCCCCCCGCCCAGCCAGCATCGCAGGGCTCAGGACCAACCGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224087 representing NM_183422
Red=Cloning site Green=Tags(s) MHQPPESTAAAAAAADISARKMAHPAMFPRRGSGSGSASALNAAGTGVGSNATSSEDFPPPSLLQPPPPA ASSTSGPQPPPPQSLNLLSQAQLQAQPLAPGGTQMKKKSGFQITSVTPAQISASISSNNSIAEDTESYDD LDESHTEDLSSSEILDVSLSRATDLGEPERSSSEETLNNFQEAETPGAVSPNQPHLPQPHLPHLPQQNVV INGNAHPHHLHHHHQIHHGHHLQHGHHHPSHVAVASASITGGPPSSPVSRKLSTTGSSDSITPVAPTSAV SSSGSPASVMTNMRAPSTTGGIGINSVTGTSTVNNVNITAVGSFNPNVTSSMLGNVNISTSNIPSAAGVS VGPGVTSGVNVNILSGMGNGTISSSAAVSSVPNAAAGMTGGSVSSQQQQPTVNTSRFRVVKLDSSSEPFK KGRWTCTEFYEKENAVPATEGVLINKVVETVKQNPIEVTSERESTSGSSVSSSVSTLSHYTESVGSGEMG APTVVVQQQQQQQQQQQQQPALQGVTLQQMDFGSTGPQSIPAVSIPQSISQSQISQVQLQSQELSYQQKQ GLQPVPLQATMSAATGIQPSPVNVVGVTSALGQQPSISSLAQPQLPYSQAAPPVQTPLPGAPPPQQLQYG QQQPMVSTQMAPGHVKSVTQNPASEYVQQQPILQTAMSSGQPSSAGVGAGTTVIPVAQPQGIQLPVQPTA VPAQPAGASVQPVGQAPAAVSAVPTGSQIANIGQQANIPTAVQQPSTQVPPSVIQQGAPPSSQVVPPAQT GIIHQGVQTSAPSLPQQLVIASQSSLLTVPPQPQGVEPVAQGIVSQQLPAVSSLPSASSISVTSQVSSTG PSGMPSAPTNLVPPQNIAQTPATQNGNLVQSVSQPPLIATNTNLPLAQQIPLSSTQFSAQSLAQAIGSQI EDARRAAEPSLVGLPQTISGDSGGMSAVSDGSSSSLAASASLFPLKVLPLTTPLVDGEDESSSGASVVAI DNKIEQAMDLVKSHLMYAVREEVEVLKEQIKELIEKNSQLEQENNLLKTLASPEQLAQFQAQLQTGSPPA TTQPQGTTQPPAQPASQGSGPTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_183422 |
ORF Size | 3219 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_183422.4 |
RefSeq Size | 6196 bp |
RefSeq ORF | 3222 bp |
Locus ID | 8848 |
UniProt ID | Q15714 |
Protein Families | Transcription Factors |
MW | 109.7 kDa |
Gene Summary | This gene encodes a member of the TSC22 domain family of leucine zipper transcription factors. The encoded protein is stimulated by transforming growth factor beta, and regulates the transcription of multiple genes including C-type natriuretic peptide. The encoded protein may play a critical role in tumor suppression through the induction of cancer cell apoptosis, and a single nucleotide polymorphism in the promoter of this gene has been associated with diabetic nephropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224087L3 | Lenti-ORF clone of TSC22D1 (Myc-DDK-tagged)-Human TSC22 domain family, member 1 (TSC22D1), transcript variant 1 |
CNY 12,640.00 |
|
RC224087L4 | Lenti-ORF clone of TSC22D1 (mGFP-tagged)-Human TSC22 domain family, member 1 (TSC22D1), transcript variant 1 |
CNY 12,640.00 |
|
RG224087 | TSC22D1 (tGFP-tagged) - Human TSC22 domain family, member 1 (TSC22D1), transcript variant 1 |
CNY 13,552.00 |
|
SC107708 | TSC22D1 (untagged)-Human TSC22 domain family, member 1 (TSC22D1), transcript variant 1 |
CNY 10,450.00 |
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