TIAL1 (NM_001033925) Human Tagged ORF Clone

CAT#: RC224019

TIAL1 (Myc-DDK-tagged)-Human TIA1 cytotoxic granule-associated RNA binding protein-like 1 (TIAL1), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001033925" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TIAL1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms TCBP; TIAR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224019 representing NM_001033925
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAGACGACGGGCAGCCCCGGACTCTATACGTAGGTAACCTTTCCAGAGATGTGACAGAAGTCC
TTATACTTCAGTTGTTCAGTCAGATTGGACCCTGTAAAAGCTGTAAAATGATAACAGAGCAACCCGATAG
CAGAAGGGTCAACTCTTCTGTTGGATTTTCTGTTTTGCAGCATACAAGCAATGACCCATATTGCTTTGTG
GAATTTTATGAACACAGAGATGCAGCTGCTGCATTAGCTGCTATGAATGGGAGAAAAATTTTGGGAAAGG
AGGTCAAAGTAAACTGGGCAACCACACCAAGTAGCCAGAAAAAAGATACTTCCAATCACTTCCATGTGTT
TGTTGGGGATTTGAGTCCAGAAATTACAACAGAAGATATCAAATCAGCATTTGCCCCCTTTGGTAAAATA
TCGGATGCCCGGGTAGTTAAAGACATGGCAACTGGAAAATCCAAAGGCTATGGTTTTGTATCTTTTTATA
ACAAACTGGATGCAGAAAATGCGATTGTGCATATGGGCGGTCAGTGGTTGGGTGGTCGTCAAATCCGAAC
CAATTGGGCCACTCGTAAACCACCTGCACCTAAAAGTACACAAGAAAACAACACTAAGCAGTTGAGATTT
GAAGATGTAGTAAACCAGTCAAGTCCAAAAAATTGTACTGTGTACTGTGGAGGAATTGCGTCTGGGTTAA
CAGATCAGCTTATGAGACAGACATTCTCACCATTTGGACAAATTATGGAAATAAGAGTTTTCCCAGAAAA
GGGCTATTCATTTGTCAGATTTTCAACCCATGAAAGTGCAGCCCATGCCATTGTTTCGGTGAACGGTACT
ACGATTGAAGGACATGTGGTTAAATGCTATTGGGGTAAAGAATCTCCTGATATGACTAAAAACTTCCAAC
AGGTTGACTATAGTCAATGGGGCCAATGGAGCCAAGTGTATGGAAACCCACAACAGTATGGACAGTATAT
GGCAAATGGGTGGCAAGTACCGCCTTATGGAGTATACGGGCAACCATGGAATCAACAAGGATTTGGAGTA
GATCAATCACCTTCTGCTGCTTGGATGGGTGGATTTGGTGCTCAGCCTCCCCAAGGACAAGCTCCTCCCC
CTGTAATACCTCCTCCTAACCAAGCCGGATATGGTATGGCAAGTTACCAAACACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224019 representing NM_001033925
Red=Cloning site Green=Tags(s)

MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEQPDSRRVNSSVGFSVLQHTSNDPYCFV
EFYEHRDAAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPFGKI
SDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENNTKQLRF
EDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGT
TIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVYGNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGV
DQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASYQTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001033925
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001033925.1, NP_001029097.1
RefSeq Size 3945 bp
RefSeq ORF 1179 bp
Locus ID 7073
UniProt ID Q01085
Protein Families Druggable Genome, Transcription Factors
MW 43.3 kDa
Gene Summary The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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