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FADS2 (NM_004265) Human Tagged ORF Clone
CAT＃: RC223780
FADS2 (Myc-DDK-tagged)-Human fatty acid desaturase 2 (FADS2)
ORF Plasmid: tGFP
"NM_004265" in other vectors (6)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D6D; DES6; FADSD6; LLCDL2; SLL0262; TU13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223780 representing NM_004265
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGGAGGGAACCAGGGCGAGGGGGCCGCCGAGCGCGAGGTGTCGGTGCCCACCTTCAGCTGGG AGGAGATTCAGAAGCATAACCTGCGCACCGACAGGTGGCTGGTCATTGACCGCAAGGTTTACAACATCAC CAAATGGTCCATCCAGCACCCGGGGGGCCAGCGGGTCATCGGGCACTACGCTGGAGAAGATGCAACGGAT GCCTTCCGCGCCTTCCACCCTGACCTGGAATTCGTGGGCAAGTTCTTGAAACCCCTGCTGATTGGTGAAC TGGCCCCGGAGGAGCCCAGCCAGGACCACGGCAAGAACTCAAAGATCACTGAGGACTTCCGGGCCCTGAG GAAGACGGCTGAGGACATGAACCTGTTCAAGACCAACCACGTGTTCTTCCTCCTCCTCCTGGCCCACATC ATCGCCCTGGAGAGCATTGCATGGTTCACTGTCTTTTACTTTGGCAATGGCTGGATTCCTACCCTCATCA CGGCCTTTGTCCTTGCTACCTCTCAGGCCCAAGCTGGATGGCTGCAACATGATTATGGCCACCTGTCTGT CTACAGAAAACCCAAGTGGAACCACCTTGTCCACAAATTCGTCATTGGCCACTTAAAGGGTGCCTCTGCC AACTGGTGGAATCATCGCCACTTCCAGCACCACGCCAAGCCTAACATCTTCCACAAGGATCCCGATGTGA ACATGCTGCACGTGTTTGTTCTGGGCGAATGGCAGCCCATCGAGTACGGCAAGAAGAAGCTGAAATACCT GCCCTACAATCACCAGCACGAATACTTCTTCCTGATTGGGCCGCCGCTGCTCATCCCCATGTATTTCCAG TACCAGATCATCATGACCATGATCGTCCATAAGAACTGGGTGGACCTGGCCTGGGCCGTCAGCTACTACA TCCGGTTCTTCATCACCTACATCCCTTTCTACGGCATCCTGGGAGCCCTCCTTTTCCTCAACTTCATCAG GTTCCTGGAGAGCCACTGGTTTGTGTGGGTCACACAGATGAATCACATCGTCATGGAGATTGACCAGGAG GCCTACCGTGACTGGTTCAGTAGCCAGCTGACAGCCACCTGCAACGTGGAGCAGTCCTTCTTCAACGACT GGTTCAGTGGACACCTTAACTTCCAGATTGAGCACCACCTCTTCCCCACCATGCCCCGGCACAACTTACA CAAGATCGCCCCGCTGGTGAAGTCTCTATGTGCCAAGCATGGCATTGAATACCAGGAGAAGCCGCTACTG AGGGCCCTGCTGGACATCATCAGGTCCCTGAAGAAGTCTGGGAAGCTGTGGCTGGACGCCTACCTTCACA AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223780 representing NM_004265
Red=Cloning site Green=Tags(s) MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATD AFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHI IALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASA NWWNHRHFQHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQ YQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQE AYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLL RALLDIIRSLKKSGKLWLDAYLHK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004265 |
| ORF Size | 1332 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004265.4 |
| RefSeq Size | 3149 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 9415 |
| UniProt ID | O95864 |
| Domains | heme_1, FA_desaturase |
| Protein Families | Transmembrane |
| Protein Pathways | alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, PPAR signaling pathway |
| MW | 52.1 kDa |
| Gene Summary | The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Polyunsaturated Fatty Acid Desaturation Is a Mechanism for Glycolytic NAD+ Recycling
,Kim, W;Deik, A;Gonzalez, C;Gonzalez, ME;Fu, F;Ferrari, M;Churchhouse, CL;Florez, JC;Jacobs, SBR;Clish, CB;Rhee, EP;,
Cell Metab.
,PubMed ID 30686744
[FADS2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223780L1 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC223780L2 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), mGFP tagged |
CNY 7,888.00 |
|
| RC223780L3 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC223780L4 | Lenti ORF clone of Human fatty acid desaturase 2 (FADS2), mGFP tagged |
CNY 5,890.00 |
|
| RG223780 | FADS2 (tGFP-tagged) - Human fatty acid desaturase 2 (FADS2) |
CNY 4,370.00 |
|
| SC117468 | FADS2 (untagged)-Human fatty acid desaturase 2 (FADS2) |
CNY 5,488.00 |
