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LXR alpha (NR1H3) (NM_005693) Human Tagged ORF Clone
CAT＃: RC223767
NR1H3 (Myc-DDK-tagged)-Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1
ORF Plasmid: tGFP
"NM_005693" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LXR-a; LXRA; RLD-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223767 representing NM_005693
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTGTGGCTGGGGGCCCCTGTGCCTGACATTCCTCCTGACTCTGCGGTGGAGCTGTGGAAGCCAG GCGCACAGGATGCAAGCAGCCAGGCCCAGGGAGGCAGCAGCTGCATCCTCAGAGAGGAAGCCAGGATGCC CCACTCTGCTGGGGGTACTGCAGGGGTGGGGCTGGAGGCTGCAGAGCCCACAGCCCTGCTCACCAGGGCA GAGCCCCCTTCAGAACCCACAGAGATCCGTCCACAAAAGCGGAAAAAGGGGCCAGCCCCCAAAATGCTGG GGAACGAGCTATGCAGCGTGTGTGGGGACAAGGCCTCGGGCTTCCACTACAATGTTCTGAGCTGCGAGGG CTGCAAGGGATTCTTCCGCCGCAGCGTCATCAAGGGAGCGCACTACATCTGCCACAGTGGCGGCCACTGC CCCATGGACACCTACATGCGTCGCAAGTGCCAGGAGTGTCGGCTTCGCAAATGCCGTCAGGCTGGCATGC GGGAGGAGTGTGTCCTGTCAGAAGAACAGATCCGCCTGAAGAAACTGAAGCGGCAAGAGGAGGAACAGGC TCATGCCACATCCTTGCCCCCCAGGGCTTCCTCACCCCCCCAAATCCTGCCCCAGCTCAGCCCGGAACAA CTGGGCATGATCGAGAAGCTCGTCGCTGCCCAGCAACAGTGTAACCGGCGCTCCTTTTCTGACCGGCTTC GAGTCACGCCTTGGCCCATGGCACCAGATCCCCATAGCCGGGAGGCCCGTCAGCAGCGCTTTGCCCACTT CACTGAGCTGGCCATCGTCTCTGTGCAGGAGATAGTTGACTTTGCTAAACAGCTACCCGGCTTCCTGCAG CTCAGCCGGGAGGACCAGATTGCCCTGCTGAAGACCTCTGCGATCGAGGTGATGCTTCTGGAGACATCTC GGAGGTACAACCCTGGGAGTGAGAGTATCACCTTCCTCAAGGATTTCAGTTATAACCGGGAAGACTTTGC CAAAGCAGGGCTGCAAGTGGAATTCATCAACCCCATCTTCGAGTTCTCCAGGGCCATGAATGAGCTGCAA CTCAATGATGCCGAGTTTGCCTTGCTCATTGCTATCAGCATCTTCTCTGCAGACCGGCCCAACGTGCAGG ACCAGCTCCAGGTAGAGAGGCTGCAGCACACATATGTGGAAGCCCTGCATGCCTACGTCTCCATCCACCA TCCCCATGACCGACTGATGTTCCCACGGATGCTAATGAAACTGGTGAGCCTCCGGACCCTGAGCAGCGTC CACTCAGAGCAAGTGTTTGCACTGCGTCTGCAGGACAAAAAGCTCCCACCGCTGCTCTCTGAGATCTGGG ATGTGCACGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223767 representing NM_005693
Red=Cloning site Green=Tags(s) MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGGSSCILREEARMPHSAGGTAGVGLEAAEPTALLTRA EPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHC PMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQ LGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQ LSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQ LNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSV HSEQVFALRLQDKKLPPLLSEIWDVHE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005693 |
| ORF Size | 1341 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005693.4 |
| RefSeq Size | 1528 bp |
| RefSeq ORF | 1344 bp |
| Locus ID | 10062 |
| UniProt ID | Q13133 |
| Domains | HOLI, zf-C4 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| Protein Pathways | PPAR signaling pathway |
| MW | 50.2 kDa |
| Gene Summary | The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pregnane X Receptor Activator Rifampin Increases Blood Pressure and Stimulates Plasma Renin Activity
,Hassani-Nezhad-Gashti, F;Salonurmi, T;Hautajärvi, H;Rysä, J;Hakkola, J;Hukkanen, J;,
Clin. Pharmacol. Ther.
,PubMed ID 32344455
[LXR alpha]
|
|
Receptor-binding affinities of bisphenol A and its next-generation analogs for human nuclear receptors
,Liu, X;Sakai, H;Nishigori, M;Suyama, K;Nawaji, T;Ikeda, S;Nishigouchi, M;Okada, H;Matsushima, A;Nose, T;Shimohigashi, M;Shimohigashi, Y;,
Toxicol. Appl. Pharmacol.
,PubMed ID 31195007
[LXR alpha]
|
|
The transcription factor MafB promotes anti-inflammatory M2 polarization and cholesterol efflux in macrophages
,Kim, H;,
Sci Rep
,PubMed ID 28790455
[LXR alpha]
|
|
AMPK activates LXRa and ABCA1 expression in human macrophages
,Kemmerer, M;Wittig, I;Richter, F;Brüne, B;Namgaladze, D;,
Int. J. Biochem. Cell Biol.
,PubMed ID 27343431
[LXR alpha]
|
|
Emodin isolated from Polygoni Multiflori Ramulus inhibits melanogenesis through the liver X receptor-mediated pathway
,Kim, MO;Park, YS;Nho, YH;Yun, SK;Kim, Y;Jung, E;Paik, JK;Kim, M;Cho, IH;Lee, J;,
Chem. Biol. Interact.
,PubMed ID 26972667
[LXR alpha]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223767L1 | Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC223767L2 | Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223767L3 | Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC223767L4 | Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG223767 | NR1H3 (tGFP-tagged) - Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1 |
CNY 5,256.00 |
|
| SC116564 | NR1H3 (untagged)-Human nuclear receptor subfamily 1, group H, member 3 (NR1H3), transcript variant 1 |
CNY 5,488.00 |
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