CCDC83 (NM_173556) Human Tagged ORF Clone

CAT#: RC223659

CCDC83 (Myc-DDK-tagged)-Human coiled-coil domain containing 83 (CCDC83)

ORF Plasmid: DDK tGFP



  "NM_173556" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal antibody to CCDC83 (coiled-coil domain containing 83)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CT148; HSD9; KP-CoT-23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223659 representing NM_173556
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAACTCAGGGAAAGCAAATAAAAAGGATACACATGACGGGCCACCAAAAGAAATTAAACTGCCTA
CCAGTGAAGCACTTCTAGACTATCAATGTCAAATAAAGGAAGATGCCGTGGAGCAATTCATGTTTCAAAT
AAAGACACTTAGGAAAAAGAACCAAAAATATCATGAAAGAAATAGCCGCTTAAAAGAAGAACAGATTTGG
CACATACGGCATCTACTAAAGGAACTGAGTGAAGAGAAGGCAGAGGGATTGCCAGTTGTAACAAGAGAGG
ATGTTGAAGAAGCGATGAAGGAAAAATGGAAGTTTGAAAGAGACCAGGAAAAAAACTTGAGAGATATGCG
CATGCAAATAAGTAATGCTGAGAAACTATTTCTTGAGAAACTCAGTGAAAAGGAATATTGGGAGGAGTAC
AAGAATGTAGGGAGTGAACGACATGCTAAACTCATTACCTCCTTACAAAATGACATCAACACAGTTAAAG
AGAATGCAGAGAAAATGTCAGAACACTATAAAATCACTCTGGAAGATACTAGAAAGAAAATAATCAAGGA
AACTTTGTTGCAACTGGACCAAAAGAAGGAATGGGCCACACAGAATGCTGTAAAGCTCATTGACAAGGGC
AGTTATCTAGAGATCTGGGAGAATGACTGGCTCAAAAAAGAGGTTGCAATTCACAGGAAGGAAGTTGAAG
AATTAAAAAATGCTATTCATGAACTGGAAGCAGAAAATTTGGTGCTTATTGATCAACTATCCAACTGTAG
ACTTGTGGATCTCAAGATACCCAGGTATCCAGTGCTACATTCCTGTCCCACCTCTAATCCTCGTCATCTG
CTGCTGCTGCCTTTGGAATCATGTCTAATCTCTGCCAGGCGTTGCTGGCGACTATATCTTACCCAAGCTG
CTGGACTAGAAGTGCCACCTGAAGAAATGTCTTTGGAATTGCCAGAAACACATATAGAAGAGAAGTCAGA
ATTGCAACCCACAGAAGTAGAAAGTAGAGACTTGATGTCCTCATCAGATGAGAGCACTATCTTACATCTT
AGTCATGAAAATAGCATCGAAGATCTCCAGTATGTGAAGATAGATAAAGAGGAAAACTCAGGCACAGAGT
TTGGGGACACTGATATGAAGTACTTACTATATGAGGATGAGAAGGATTTCAAGGATTATGTAAACTTGGG
CCCCCTGGGAGTGAAGCTTATGAGTGTGGAGAGCAAGAAAATGCCCATTCATTTTCAAGAGAAGGAAATT
CCAGTCAAACTCTATAAAGATGTCAGGAGCCCAGAAAGCCACATCACATATAAGATGATGAAGTCTTTTC
TC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223659 representing NM_173556
Red=Cloning site Green=Tags(s)

MENSGKANKKDTHDGPPKEIKLPTSEALLDYQCQIKEDAVEQFMFQIKTLRKKNQKYHERNSRLKEEQIW
HIRHLLKELSEEKAEGLPVVTREDVEEAMKEKWKFERDQEKNLRDMRMQISNAEKLFLEKLSEKEYWEEY
KNVGSERHAKLITSLQNDINTVKENAEKMSEHYKITLEDTRKKIIKETLLQLDQKKEWATQNAVKLIDKG
SYLEIWENDWLKKEVAIHRKEVEELKNAIHELEAENLVLIDQLSNCRLVDLKIPRYPVLHSCPTSNPRHL
LLLPLESCLISARRCWRLYLTQAAGLEVPPEEMSLELPETHIEEKSELQPTEVESRDLMSSSDESTILHL
SHENSIEDLQYVKIDKEENSGTEFGDTDMKYLLYEDEKDFKDYVNLGPLGVKLMSVESKKMPIHFQEKEI
PVKLYKDVRSPESHITYKMMKSFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173556
ORF Size 1332 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173556.5
RefSeq Size 2346 bp
RefSeq ORF 1335 bp
Locus ID 220047
UniProt ID Q8IWF9
MW 52.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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