United States
Germany
Japan
United Kingdom
China
SIK3 (NM_025164) Human Tagged ORF Clone
CAT＃: RC223406
SIK3 (Myc-DDK-tagged)-Human SIK family kinase 3 (SIK3)
ORF Plasmid: tGFP
"NM_025164" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 8,792.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | L19; QSK; SEMDK; SIK-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223406 representing NM_025164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCCCGTATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGC GGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATCAAGATCATAGATAAGACCCAGCTGGATGAAGA AAACTTGAAGAAGATTTTCCGGGAAGTTCAAATTATGAAGATGCTTTGCCACCCCCATATCATCAGGCTC TACCAGGTTATGGAGACAGAACGGATGATTTATCTGGTGACAGAATATGCTAGTGGAGGGGAAATATTTG ACCACCTGGTGGCCCATGGTAGAATGGCAGAAAAGGAGGCACGTCGGAAGTTCAAACAGATCGTCACAGC TGTCTATTTTTGTCACTGTCGGAACATTGTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCC AATCTGAATATCAAAATAGCAGATTTTGGTTTCAGTAACCTCTTCACTCCTGGGCAGCTGCTGAAGACCT GGTGTGGCAGCCCTCCCTATGCTGCACCTGAACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGA CATCTGGAGCCTTGGAGTTGTCCTCTACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAGCACACTG CAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTCCGCATCCCATTTTTTATGTCCACAGAATGTGAGC ATTTGATCCGCCATATGTTGGTGTTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAA GTGGATGAAGCTAGGGGACGCCGATCCCAACTTTGACAGGTTAATAGCTGAATGCCAACAACTAAAGGAA GAAAGACAGGTGGACCCCCTGAATGAGGATGTCCTCTTGGCCATGGAGGACATGGGACTGGACAAAGAAC AGACACTGCAGTCATTAAGATCAGATGCCTATGATCACTATAGTGCAATCTACAGCCTGCTGTGTGATCG ACATAAGAGACATAAAACCCTGCGTCTCGGAGCACTTCCTAGCATGCCCCGAGCCCTGGCCTTTCAAGCA CCAGTCAATATCCAGGCGGAGCAGGCAGGTACTGCTATGAACATCAGCGTTCCCCAGGTGCAGCTGATCA ACCCAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAATTTGGACAGTGATGAGGGTGAAGAGCCTTC CCCTGAAGCATTGGTGCGCTATTTGTCAATGAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAA GTTATGGAAGATCTGCAGAAGCTCCTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCATTCCTGCAGG TGGCCCCTAATGTGAACTTCATGCACAACCTGTTGCCTATGCAAAACTTGCAACCAACCGGGCAACTTGA GTACAAGGAGCAGTCTCTCCTACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTTGGCCGG AGGGCATCAGATGGAGGAGCCAACATCCAACTGCATGCCCAGCAGCTGCTGAAGCGCCCACGGGGACCCT CTCCGCTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGA GCCAGACCAGGAAGCTGTGCAGAGCTCTACCTACAAGGACTCCAACACTCTGCACCTCCCTACGGAGCGT TTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAA TGGGCAACAACAGCAGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCA GATTGATGAAAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTATACCAGCAGGAGCAGCACCATCAA ATTCTCCAGCAACAAATTCAAGACTCTATCTGTCCTCCTCAGCCATCTCCACCTCTTCAGGCTGCATGTG AAAATCAGCCAGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCCCACCCCCCAA CCACCCCAACAACCATCTCTTCAGGCAGCCCAGTAATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAG CCTCACGGGGCTGCATCTTCTTCCCAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGCAAC CTGAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACA GCAGGTCACCATCCAAGTCCAAGAGCCTGTTGACATGCTCAGCAACATGCCAGGCACAGCTGCAGGCTCC AGTGGGCGCGGCATCTCCATCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCACCGTACCAACCTGATGG CCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCTGAGTGCTGACAGTGCAGAGGCTCACAGCTT GAACGTGAATCGGTTCTCCCCTGCTAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAG TCCCGGGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTCTCTCCAACCCAAGCCCTGAAAG TCCCTCCACTTGACCAATTCCCCACCTTCCCTCCCAGTGCACATCAGCAGCCGCCACACTATACCACGTC GGCACTACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGTACCCCACATC CTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACCGGCCACTCGGACATCCGGCTGCCCCCAACAGAGT TTGCACAGCTCATTAAAAGGCAGCAGCAACAACGGCAGCAGCAGCAGCAACAGCAGCAACAGCAAGAATA CCAGGAACTGTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGC ATGACAGAGCGCCAGGCTTTATCTTATCAAAATGCTGACTCTTATCACCATCACACCAGCCCCCAGCATC TGCTACAAATCAGGGCACAAGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCCACGGGTATGCTCA CCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAGGACTGCTCGTGTGAGGGGGCCAAGGATGGCTTC CAAGACAGTAAGAGTTCAAGTACATTGACCAAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTG GACCTGGGGACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCCCAAGAATTGGGGATACATCCCTATGG TCATCAGCCAACTGCTGCATTCAGTAAAAATAAGGTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATG GATAGAAGTTCTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCAGCACGCCCCT CCGTCCATGAGCACCACAGGCCCCGGGCCCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCTTA TGTACAGCTGGATAACTTGCCAGGAATGAGTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGGATGTCG GATGCAGTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGATGGGGAAAATGAGGAATGTG GGGCAAGCCTGGGAGGTCATGAGCACCCAGACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTA TCCATCTACGTGTATTACAGACATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCAG GCAGGCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223406 representing NM_025164
Red=Cloning site Green=Tags(s) MPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRL YQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDA NLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTL QNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKE ERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQA PVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTE VMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGR RASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQSSTYKDSNTLHLPTER FSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQ ILQQQIQDSICPPQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQ PHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGS SGRGISISPSAGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQ SRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPDYTRHQQVPHI LQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQS MTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGF QDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCM DRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMS DAVLSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQ AGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025164 |
| ORF Size | 3789 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025164.3, NP_079440.2 |
| RefSeq Size | 6072 bp |
| RefSeq ORF | 3966 bp |
| Locus ID | 23387 |
| UniProt ID | Q9Y2K2 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 139.8 kDa |
| Gene Summary | Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed:30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
New age-related hearing loss candidate genes in humans: an ongoing challenge
,Di Stazio, M;Morgan, A;Brumat, M;Bassani, S;Dell'Orco, D;Marino, V;Garagnani, P;Giuliani, C;Gasparini, P;Girotto, G;,
Gene
,PubMed ID 32173538
[SIK3]
|
|
The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling
,Csukasi, F;Duran, I;Barad, M;Barta, T;Gudernova, I;Trantirek, L;Martin, JH;Kuo, CY;Woods, J;Lee, H;Cohn, DH;Krejci, P;Krakow, D;,
Sci Transl Med
,PubMed ID 30232230
[SIK3]
|
|
Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation
,Amara, S;Majors, C;Roy, B;Hill, S;Rose, KL;Myles, EL;Tiriveedhi, V;,
PLoS ONE
,PubMed ID 28658303
[SIK3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223406L1 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), Myc-DDK-tagged |
CNY 11,192.00 |
|
| RC223406L2 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), mGFP tagged |
CNY 14,540.00 |
|
| RC223406L3 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), Myc-DDK-tagged |
CNY 11,192.00 |
|
| RC223406L4 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), mGFP tagged |
CNY 11,192.00 |
|
| RG223406 | SIK3 (tGFP-tagged) - Human SIK family kinase 3 (SIK3) |
CNY 13,870.00 |
|
| SC309935 | SIK3 (untagged)-Human SIK family kinase 3 (SIK3) |
CNY 24,320.00 |
