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Anoctamin 3 (ANO3) (NM_031418) Human Tagged ORF Clone
CAT＃: RC223382
- TrueORF®
ANO3 (Myc-DDK-tagged)-Human anoctamin 3 (ANO3)
ORF Plasmid: tGFP
"NM_031418" in other vectors (6)
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CNY 10,776.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C11orf25; DYT23; DYT24; GENX-3947; TMEM16C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223382 representing NM_031418
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCACCATTCAGGCTCCATTCAGTCCTTTAAACAGCAAAAAGGTATGAATATAAGCAAGAGTGAGA TAACAAAAGAAACTTCGTTAAAACCGTCTCGGAGATCCCTGCCTTGCCTCGCCCAGAGCTACGCTTACTC AAAGAGCTTGAGCCAGTCTACTTCCCTCTTCCAGTCAACCGAGAGTGAATCTCAGGCTCCCACATCTATA ACCTTAATCTCCACTGACAAAGCAGAGCAAGTTAATACTGAGGAGAATAAAAACGACTCTGTGCTGAGAT GTTCATTTGCTGACCTCAGCGATTTTTGTTTGGCCCTAGGAAAAGATAAGGATTACACGGATGAATCAGA ACACGCTACTTATGACCGATCTCGTCTCATTAATGACTTTGTTATCAAAGATAAATCTGAATTCAAGACA AAATTATCTAAGAATGACATGAATTACATAGCATCCAGTGGACCTCTGTTCAAAGATGGCAAAAAGAGAA TTGATTACATCTTGGTTTATAGAAAGACAAATATACAATATGATAAAAGAAACACATTTGAAAAGAACCT CAGAGCAGAAGGCTTGATGTTGGAGAAGGAGCCAGCTATTGCAAGCCCCGATATCATGTTTATTAAAATT CACATTCCATGGGACACGCTGTGCAAGTATGCAGAGAGGCTGAATATCAGGATGCCCTTCAGGAAAAAAT GCTATTACACTGACGGGAGGAGCAAATCAATGGGCAGGATGCAAACTTATTTTAGAAGAATCAAAAACTG GATGGCCCAAAACCCAATGGTTCTTGACAAGTCAGCTTTTCCAGACCTAGAGGAGTCAGACTGCTATACT GGCCCCTTCAGCCGTGCACGGATTCACCACTTCATAATAAATAATAAAGACACCTTCTTCAGCAATGCTA CTCGAAGCAGAATAGTCTATCACATGCTGGAACGCACCAAATATGAAAATGGAATATCAAAAGTGGGTAT CCGTAAACTTATAAACAATGGCTCATACATAGCAGCGTTTCCACCACATGAGGGAGCCTACAAAAGTAGC CAGCCCATTAAAACCCATGGACCTCAGAATAACAGACATCTATTATATGAGCGCTGGGCACGCTGGGGAA TGTGGTATAAGCATCAGCCTCTGGATTTAATCAGGCTATACTTTGGTGAGAAGATTGGACTATACTTTGC TTGGCTGGGATGGTATACTGGAATGTTGATTCCTGCAGCAATTGTTGGTTTGTGCGTTTTCTTCTATGGA TTATTTACAATGAATAATAGTCAAGTAAGCCAAGAAATTTGTAAAGCCACTGAAGTCTTTATGTGCCCTC TCTGTGACAAGAACTGCTCCCTGCAGAGACTCAACGACAGCTGTATCTATGCCAAGGTGACATATTTGTT CGATAATGGAGGGACAGTCTTCTTTGCTATTTTTATGGCAATATGGGCCACAGTCTTCCTGGAGTTTTGG AAAAGGAGAAGGAGTATACTGACCTATACTTGGGACCTTATCGAATGGGAAGAAGAGGAGGAAACACTTC GTCCCCAGTTTGAAGCCAAGTATTACAAGATGGAGATTGTAAATCCCATCACGGGAAAACCTGAACCACA TCAGCCTTCCTCAGACAAAGTCACTCGTCTTCTTGTTTCTGTCTCAGGAATATTCTTCATGATATCCTTG GTGATCACTGCAGTGTTTGGAGTTGTGGTGTACCGCCTGGTTGTCATGGAACAGTTTGCATCATTCAAGT GGAATTTCATCAAACAATACTGGCAGTTTGCAACATCTGCTGCTGCTGTCTGTATCAATTTCATAATCAT TATGTTGCTGAATCTTGCTTATGAAAAAATTGCTTACCTCCTCACCAATTTAGAATATCCTCGAACAGAA TCAGAGTGGGAAAACAGCTTCGCCCTGAAGATGTTCCTCTTCCAGTTTGTCAATTTAAACAGTTCCATCT TCTATATCGCTTTCTTTTTGGGAAGATTCGTAGGCCACCCAGGAAAATACAATAAACTTTTTGACCGGTG GAGACTGGAGGAATGTCATCCTAGTGGCTGTTTGATAGACCTCTGCCTCCAGATGGGTGTCATCATGTTT TTGAAGCAAATATGGAACAACTTCATGGAACTAGGATACCCGTTGATCCAGAACTGGTGGTCACGACATA AAATCAAGCGGGGAATACATGATGCTTCCATACCTCAGTGGGAAAATGATTGGAATCTGCAGCCCATGAA CCTTCATGGACTGATGGATGAGTACTTAGAAATGGTTTTGCAATTTGGTTTTACCACCATCTTTGTTGCG GCTTTTCCTCTAGCCCCTCTTTTGGCTTTGTTAAACAATATCATTGAAATCAGGCTGGATGCATACAAAT TTGTCACTCAATGGCGGAGGCCTTTGCCAGCCCGAGCAACTGACATAGGTATCTGGCTTGGAATTCTCGA AGGAATCGGTATATTGGCTGTGATCACCAATGCATTTGTAATTGCTATTACTTCTGATTACATCCCACGT TTTGTTTATGAATACAAATATGGCCCCTGTGCAAATCATGTAGAACCAAGTGAAAATTGCTTGAAGGGAT ATGTCAACAATAGCCTATCCTTCTTTGACCTGAGTGAGCTTGGTATGGGAAAATCTGGTTATTGCAGGTA CAGAGACTACAGAGGCCCGCCCTGGAGTTCCAAACCCTATGAGTTTACTTTACAATACTGGCATATCCTT GCTGCTAGATTGGCCTTCATTATTGTGTTTGAGCACCTTGTTTTTGGGATTAAGTCATTCATCGCATACC TGATTCCAGACGTACCAAAGGGTCTACATGACCGAATACGACGAGAGAAGTACTTAGTTCAAGAAATGAT GTATGAGGCTGAACTGGAACATTTGCAACAACAACGGAGAAAAAGTGGTCAGCCTGTTCACCATGAATGG CCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223382 representing NM_031418
Red=Cloning site Green=Tags(s) MVHHSGSIQSFKQQKGMNISKSEITKETSLKPSRRSLPCLAQSYAYSKSLSQSTSLFQSTESESQAPTSI TLISTDKAEQVNTEENKNDSVLRCSFADLSDFCLALGKDKDYTDESEHATYDRSRLINDFVIKDKSEFKT KLSKNDMNYIASSGPLFKDGKKRIDYILVYRKTNIQYDKRNTFEKNLRAEGLMLEKEPAIASPDIMFIKI HIPWDTLCKYAERLNIRMPFRKKCYYTDGRSKSMGRMQTYFRRIKNWMAQNPMVLDKSAFPDLEESDCYT GPFSRARIHHFIINNKDTFFSNATRSRIVYHMLERTKYENGISKVGIRKLINNGSYIAAFPPHEGAYKSS QPIKTHGPQNNRHLLYERWARWGMWYKHQPLDLIRLYFGEKIGLYFAWLGWYTGMLIPAAIVGLCVFFYG LFTMNNSQVSQEICKATEVFMCPLCDKNCSLQRLNDSCIYAKVTYLFDNGGTVFFAIFMAIWATVFLEFW KRRRSILTYTWDLIEWEEEEETLRPQFEAKYYKMEIVNPITGKPEPHQPSSDKVTRLLVSVSGIFFMISL VITAVFGVVVYRLVVMEQFASFKWNFIKQYWQFATSAAAVCINFIIIMLLNLAYEKIAYLLTNLEYPRTE SEWENSFALKMFLFQFVNLNSSIFYIAFFLGRFVGHPGKYNKLFDRWRLEECHPSGCLIDLCLQMGVIMF LKQIWNNFMELGYPLIQNWWSRHKIKRGIHDASIPQWENDWNLQPMNLHGLMDEYLEMVLQFGFTTIFVA AFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLPARATDIGIWLGILEGIGILAVITNAFVIAITSDYIPR FVYEYKYGPCANHVEPSENCLKGYVNNSLSFFDLSELGMGKSGYCRYRDYRGPPWSSKPYEFTLQYWHIL AARLAFIIVFEHLVFGIKSFIAYLIPDVPKGLHDRIRREKYLVQEMMYEAELEHLQQQRRKSGQPVHHEW P TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031418 |
| ORF Size | 2943 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031418.4 |
| RefSeq Size | 6641 bp |
| RefSeq ORF | 2946 bp |
| Locus ID | 63982 |
| UniProt ID | Q9BYT9 |
| Protein Families | Transmembrane |
| MW | 114.5 kDa |
| Gene Summary | The protein encoded by this gene belongs to the TMEM16 family of predicted membrane proteins, that are also known as anoctamins. While little is known about the function of this gene, mutations in this gene have been associated with some cases of autosomal dominant craniocervical dystonia. Cells from individuals with a mutation in this gene exhibited abnormalities in endoplasmic reticulum-dependent calcium signaling. Studies in rat show that the rat ortholog of this protein interacts with, and modulates the activity of a sodium-activated potassium channel. Deletion of this gene caused increased pain sensitivity in the rat model system. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223382L1 | Lenti ORF clone of Human anoctamin 3 (ANO3), Myc-DDK-tagged |
CNY 13,176.00 |
|
| RC223382L2 | Lenti ORF clone of Human anoctamin 3 (ANO3), mGFP tagged |
CNY 11,690.00 |
|
| RC223382L3 | Lenti ORF clone of Human anoctamin 3 (ANO3), Myc-DDK-tagged |
CNY 11,690.00 |
|
| RC223382L4 | Lenti ORF clone of Human anoctamin 3 (ANO3), mGFP tagged |
CNY 11,690.00 |
|
| RG223382 | ANO3 (tGFP-tagged) - Human anoctamin 3 (ANO3) |
CNY 12,376.00 |
|
| SC305338 | ANO3 (untagged)-Human anoctamin 3 (ANO3) |
CNY 7,192.00 |
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