PIF1 (NM_025049) Human Tagged ORF Clone
CAT#: RC223376
PIF1 (Myc-DDK-tagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1)
ORF Plasmid: tGFP
"NM_025049" in other vectors (7)
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CNY 7,160.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C15orf20; PIF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223376 representing NM_025049
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTCGGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTGG AGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGTCG CAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTTCCCT CTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCGGCTCCCCGCCCACG ACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGCTTCCT GCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTG GGCCCGCGGCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGCCA CCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCTAGCACAGA AGCCCCAAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTTTCTGAGGAA CAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAGTGCAGGAACAGGGA AGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGGCACTGTGGCCACTGCCAGCACTGG GGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCATGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCT CTAGCCCAGTGTGTGGCCCTGGCCCAAAGGCCAGGCGTGCGGCAGGGCTGGCTGAACTGCCAGCGGTTGG TCATTGACGAGATCTCAATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTGGCCAGAGCTGTCCG GCAGCAGAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTGCAGCTGCCACCTGTG ACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGGTGTGTGCCAGTGACCC TGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTCTCTACTGCAGGCCGTGAGGCTAGG CAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGCCACAGCTTCCCACAAGGTGGGGCGAGATGGGATT GTGGCCACGAGGCTCTGCACCCACCAGGATGATGTGGCCCTCACCAACGAGAGGCGGCTTCAGGAGCTGC CAGGTAAGGTACACAGATTTGAGGCTATGGACAGCAACCCTGAGCTGGCCAGTACCCTGGATGCCCAGTG TCCTGTTAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTGGTGAAAAACTTATCGGTGTCT CGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCAGAAGGGAGAGGGCTACCCCAGG TGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTGGACGGTGCAGGCCACCGGGGGCCA GCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGCCTGGGCGATGTCCATCCACAAGAGCCAAGGCATG ACCCTGGATTGTGTGGAGATTTCTCTGGGCCGTGTGTTTGCCAGTGGCCAGGCCTATGTGGCCCTTTCTC GGGCCCGCAGCCTGCAGGGCCTACGTGTGCTGGACTTTGACCCCATGGCGGTTCGCTGTGACCCCCGTGT GCTGCACTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGTCTGGAGTCCCCAGATGATGATGAGGCA GCCTCAGACCAGGAGAACATGGACCCAATCCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223376 representing NM_025049
Red=Cloning site Green=Tags(s) MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFP LRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLL GPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEE QAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAP LAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPV TKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGI VATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVS RGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGM TLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEA ASDQENMDPIL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025049 |
ORF Size | 1923 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025049.4 |
RefSeq Size | 2659 bp |
RefSeq ORF | 1926 bp |
Locus ID | 80119 |
UniProt ID | Q9H611 |
MW | 69.6 kDa |
Gene Summary | This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223376L1 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), Myc-DDK-tagged |
CNY 9,560.00 |
|
RC223376L2 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), mGFP tagged |
CNY 9,560.00 |
|
RC223376L3 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC223376L4 | Lenti ORF clone of Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1), mGFP tagged |
CNY 9,560.00 |
|
RG223376 | PIF1 (tGFP-tagged) - Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
CNY 8,760.00 |
|
SC312706 | PIF1 (untagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
CNY 3,600.00 |
|
SC318097 | PIF1 (untagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1) |
CNY 7,168.00 |