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BOULE (BOLL) (NM_197970) Human Tagged ORF Clone
CAT＃: RC223217
BOLL (Myc-DDK-tagged)-Human bol, boule-like (Drosophila) (BOLL), transcript variant 1
ORF Plasmid: tGFP
"NM_197970" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BOULE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223217 representing NM_197970
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACCGAGTCCGGGCCGCAAACATCAAACCAGATGCAAACAGATTCATTATCTCCATCCCCTAATC CTGTGTCACCTGTGCCTTTGAATAACCCAACAAGTGCCCCAAGATATGGAACAGTGATCCCTAATCGCAT CTTTGTAGGAGGAATTGATTTTAAGACAAACGAAAGTGATTTAAGAAAATTTTTTTCCCAGTATGGGTCT GTGAAAGAAGTGAAGATTGTAAATGACAGAGCTGGAGTATCCAAAGGGTATGGTTTCGTCACTTTTGAAA CACAAGAAGATGCACAAAAAATTTTACAAGAGGCTGAAAAACTTAATTATAAGGATAAGAAGCTGAACAT TGGTCCAGCAATAAGAAAACAACAAGTAGGGATCCCTCGTTCTAGTATAATGCCAGCAGCTGGAACAATG TATCTAACAACTTCAACTGGATATCCTTATACTTACCATAATGGTGTTGCTTATTTTCATACTCCAGAGG TAACTTCGGTCCCACCGCCTTGGCCTTCACGTTCTGTATGTAGCTCCCCTGTGATGGTAGCTCAGCCCAT TTATCAGCAACCTGCATATCACTACCAGGCCACCACACAGTATTTACCAGGACAGTGGCAGTGGAGTGTT CCTCAGCCTTCTGCCTCTTCTGCTCCATTCTTATACCTGCAACCTTCTGAGGTTATTTATCAACCAGTGG AAATTGCACAGGATGGTGGATGTGTTCCTCCTCCACTGTCTCTGATGGAAACTTCAGTTCCAGAGCCTTA TTCTGATCATGGAGTTCAAGCAACATATCACCAGGTTTATGCTCCAAGTGCCATCACTATGCCTGCGCCT GTGATGCAGCCTGAGCCAATTAAAACAGTGTGGAGCATTCATTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223217 representing NM_197970
Red=Cloning site Green=Tags(s) METESGPQTSNQMQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGS VKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTM YLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSV PQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAP VMQPEPIKTVWSIHY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_197970 |
| ORF Size | 885 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_197970.3 |
| RefSeq Size | 2804 bp |
| RefSeq ORF | 888 bp |
| Locus ID | 66037 |
| UniProt ID | Q8N9W6 |
| MW | 32.4 kDa |
| Gene Summary | This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Oncogenic Role of BOLL in Colorectal Cancer
,Kang, KJ;Pyo, JH;Ryu, KJ;Kim, SJ;Ha, JM;Choi, K;Hong, SN;Min, BH;Chang, DK;Son, HJ;Rhee, PL;Kim, JJ;Kim, YH;,
Dig. Dis. Sci.
,PubMed ID 25605553
[BOULE]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223217L1 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC223217L2 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223217L3 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223217L4 | Lenti ORF clone of Human bol, boule-like (Drosophila) (BOLL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG223217 | BOLL (tGFP-tagged) - Human bol, boule-like (Drosophila) (BOLL), transcript variant 1 |
CNY 4,370.00 |
|
| SC307621 | BOLL (untagged)-Human bol, boule-like (Drosophila) (BOLL), transcript variant 1 |
CNY 6,270.00 |
