United States
Germany
Japan
United Kingdom
China
ASS1 (NM_000050) Human Tagged ORF Clone
CAT＃: RC223189
ASS1 (Myc-DDK-tagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 1
ORF Plasmid: tGFP
"NM_000050" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASS; CTLN1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC223189 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATCCTCGTGTGGC TGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAGAAGGAAGACTTCGAGGAAGC CAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAG GAGTTCATCTGGCCGGCCATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTG CCAGGCCCTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGG CGCCACAGGAAAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTGATGGAGTACG CAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGCATGGATGAGAACCTCATGCA CATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAACCAAGCGCCTCCAGGTCTCTACACGAAGACC CAGGACCCAGCCAAAGCCCCCAACACCCCTGACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGA AGGTGACCAACGTCAAGGATGGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGT CGCGGGCAAGCATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT ATCTACGAGACCCCAGCAGGCACCATCCTTTACCACGCTCATTTAGACATCGAGGCCTTCACCATGGACC GGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTGGTGTATACCGGTTTCTGGCA CAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAGTCCCAGGAGCGAGTGGAAGGGAAAGTGCAG GTGTCCGTCCTCAAGGGCCAGGTGTACATCCTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGC TGGTGAGCATGAACGTGCAGGGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCT CAGGCTGAAGGAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223189 protein sequence
Red=Cloning site Green=Tags(s) MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVE EFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELSCYSLAPQIK VIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKT QDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRG IYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQ VSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000050 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000050.4 |
| RefSeq Size | 1863 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 445 |
| UniProt ID | P00966 |
| Domains | Arginosuc_synth |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways |
| MW | 46.5 kDa |
| Gene Summary | The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy
,Chu, CY;Lee, YC;Hsieh, CH;Yeh, CT;Chao, TY;Chen, PH;Lin, IH;Hsieh, TH;Shih, JW;Cheng, CH;Chang, CC;Lin, PS;Huang, YL;Chen, TM;Yen, Y;Ann, DK;Kung, HJ;,
Theranostics
,PubMed ID 33664852
[ASS1]
|
|
Argininosuccinate Synthetase 1 Loss in Invasive Bladder Cancer Regulates Survival through General Control Nonderepressible 2 Kinase-Mediated Eukaryotic Initiation Factor 2α Activity and Is Targetable by Pegylated Arginine Deiminase
,Sahu, D;Gupta, S;Hau, AM;Nakashima, K;Leivo, MZ;Searles, SC;Elson, P;Bomalaski, JS;Casteel, DE;Boss, GR;Hansel, DE;,
The American Journal of Pathology
,PubMed ID 27979310
[ASS1]
|
|
Reduced argininosuccinate synthetase expression in refractory sarcomas: Impacts on therapeutic potential and drug resistance
,Kim, Y;Kobayashi, E;Kubota, D;Suehara, Y;Mukaihara, K;Akaike, K;Ito, A;Kaneko, K;Chuman, H;Kawai, A;Kitano, S;,
Oncotarget
,PubMed ID 27683125
[ASS1]
|
|
Argininosuccinate synthetase 1 suppression and arginine restriction inhibit cell migration in gastric cancer cell lines
,Shan, YS;Hsu, HP;Lai, MD;Yen, MC;Chen, WC;Fang, JH;Weng, TY;Chen, YL;,
Sci Rep
,PubMed ID 25928182
[ASS1]
|
|
ASS1 as a Novel Tumor Suppressor Gene in Myxofibrosarcomas: Aberrant Loss via Epigenetic DNA Methylation Confers Aggressive Phenotypes, Negative Prognostic Impact, and Therapeutic Relevance
,Hsuan-Ying Huang, Wen-Ren Wu, Yu-Hui Wang, Jun-Wen Wang, Fu-Min Fang, Jen-Wei Tsai, Shau-Hsuan Li, Hsiao-Chin Hung, Shih-Chen Yu, Jui Lan, Yow-Ling Shiue, Chung-His Hsing, Li-Tzong Chen, and Chien-Feng Li,
Clin. Cancer Res., Jun 2013; 19: 2861 - 2872.
,PubMed ID 23549872
[ASS1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223189L1 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC223189L2 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC223189L3 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223189L4 | Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG223189 | ASS1 (tGFP-tagged) - Human argininosuccinate synthase 1 (ASS1), transcript variant 1 |
CNY 5,256.00 |
|
| SC110830 | ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 1 |
CNY 3,656.00 |
