HIF3 alpha (HIF3A) (NM_152794) Human Tagged ORF Clone
CAT#: RC222665
- TrueORF®
HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1
ORF Plasmid: tGFP
"NM_152794" in other vectors (6)
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CNY 7,328.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222665 representing NM_152794
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGCAAGACCACAGGTCGACCACGGAGCTGCGCAAGGAAAAGTCCCGGGATGCGGCCCGCAGCC GGCGCAGCCAGGAGACCGAGGTGCTGTACCAGCTGGCTCACACGCTGCCCTTCGCCCGCGGCGTCAGCGC CCACCTGGACAAGGCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCACCGCCTCTGCGCCGCA GGGGAGTGGAACCAGGTGGGAGCAGGGGGAGAACCACTGGATGCCTGCTACCTGAAGGCCCTGGAGGGCT TCGTCATGGTGCTCACCGCCGAGGGAGACATGGCTTACCTGTCGGAGAATGTCAGCAAACACCTGGGCCT CAGTCAGCTGGAGCTCATTGGACACAGCATCTTTGATTTCATCCACCCCTGTGACCAAGAGGAGCTTCAG GACGCCCTGACCCCCCAGCAGACCCTGTCCAGGAGGAAGGTGGAGGCCCCCACGGAGCGGTGCTTCTCCT TGCGCATGAAGAGTACACTCACCAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTGGAAGGTGCT GAACTGCTCTGGACATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGCCCTGACTCA GAGCCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGGAGCCCCCAC TGGGCCGAGGGGCCTTCCTCAGCCGCCACAGCCTGGACATGAAGTTCACCTACTGTGACGACAGGATTGC AGAAGTGGCTGGCTATAGTCCCGATGACCTGATCGGCTGTTCCGCCTACGAGTACATCCACGCGCTGGAC TCCGATGCGGTCAGCAAGAGCATCCACACCTTGCTGAGCAAGGGCCAGGCAGTAACAGGGCAGTATCGCT TCCTGGCCCGGAGTGGTGGCTACCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGGACGGGGCCC CCAGTCGGAGAGTATCGTCTGTGTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTGGTGCTGTCC CTGGAGCAAACGGAGCAACACTCTCGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAAGGACACCCCTA ACCCTGGGGACAGCCTTGACACCCCTGGCCCCCGGATCCTTGCCTTCCTGCACCCGCCTTCCCTGAGCGA GGCTGCCCTGGCCGCTGACCCCCGCCGTTTCTGCAGCCCTGACCTCCGTCGCCTCCTGGGACCCATCCTG GATGGGGCTTCAGTAGCAGCCACTCCCAGCACCCCGCTGGCCACACGGCACCCCCAAAGTCCTCTTTCGG CTGATCTCCCAGATGAACTACCTGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGGGAAAGACAC TGAGGCAGTGGAGACAGATTTAGATATAGCTCAGGATGCTGATGCTCTGGATTTGGAGATGCTGGCCCCC TACATCTCCATGGATGATGACTTCCAGCTCAACGCCAGCGAGCAGCTACCCAGGGCCTACCACAGACCTC TGGGGGCTGTCCCCCGGCCCCGTGCTCGGAGCTTCCATGGCCTGTCACCTCCAGCCCTTGAGCCCTCCCT GCTACCCCGCTGGGGGAGTGACCCCCGGCTGAGCTGCTCCAGCCCTTCCAGAGGGGACCCCTCAGCATCC TCTCCCATGGCTGGGGCTCGGAAGAGGACCCTGGCCCAGAGCTCAGAGGACGAGGACGAGGGAGTGGAGC TGCTGGGAGTGAGACCTCCCAAAAGGTCCCCCAGCCCAGAACACGAAAACTTTCTGCTCTTTCCTCTCAG CCTGAGTTTCCTTCTGACAGGAGGACCAGCCCCAGGGAGCCTGCAGGACCCCAGCACCCCACTCCTGAAC CTGAATGAGCCCCTGGGCCTGGGCCCCTCACTGCTCTCTCCGTACTCAGACGAGGACACTACCCAGCCCG GGGGCCCCTTCCAGCCAAGGGCAGGCTCAGCCCAGGCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222665 representing NM_152794
Red=Cloning site Green=Tags(s) MDWQDHRSTTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLCAA GEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEELQ DALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGSPDS EPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIHALD SDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISQVEETGVVLS LEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLGPIL DGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALDLEMLAP YISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLLPRWGSDPRLSCSSPSRGDPSAS SPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEHENFLLFPLSLSFLLTGGPAPGSLQDPSTPLLN LNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_152794 |
ORF Size | 2001 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152794.4 |
RefSeq Size | 2717 bp |
RefSeq ORF | 2004 bp |
Locus ID | 64344 |
UniProt ID | Q9Y2N7 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 72.2 kDa |
Gene Summary | The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The action of HIF-3a variants on HIF-2a-HIF-1ß heterodimer formation is directly probed in live cells
,Kim, SH;Hwang, D;Park, H;Yang, EG;Chung, HS;Kim, SY;,
Exp. Cell Res.
,PubMed ID 26160453
[HIF3A]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222665L1 | Lenti-ORF clone of HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1 |
CNY 9,728.00 |
|
RC222665L2 | Lenti-ORF clone of HIF3A (mGFP-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1 |
CNY 8,930.00 |
|
RC222665L3 | Lenti-ORF clone of HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1 |
CNY 8,930.00 |
|
RC222665L4 | Lenti-ORF clone of HIF3A (mGFP-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1 |
CNY 8,930.00 |
|
RG222665 | HIF3A (tGFP-tagged) - Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1 |
CNY 7,700.00 |
|
SC306504 | HIF3A (untagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1 |
CNY 7,336.00 |
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