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PON2 (NM_000305) Human Tagged ORF Clone
CAT＃: RC222624
PON2 (Myc-DDK-tagged)-Human paraoxonase 2 (PON2), transcript variant 1
ORF Plasmid: tGFP
"NM_000305" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222624 representing NM_000305
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGCTGGTGGCTGTGGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGGCTTCTGGCAC TCAGAAATCGACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCACACTGCCACCTGATTAAAGG AATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAATGGTCTGGCTTTTTTTAGTGTGGGTCTAAAA TTCCCAGGACTCCACAGCTTTGCACCAGATAAGCCTGGAGGAATACTAATGATGGATCTAAAAGAAGAAA AACCAAGGGCACGGGAATTAAGAATCAGTCGTGGGTTTGATTTGGCCTCATTCAATCCACATGGCATCAG CACTTTCATAGACAACGATGACACAGTTTATCTCTTTGTTGTAAACCACCCAGAATTCAAGAATACAGTG GAAATTTTTAAATTTGAAGAAGCAGAAAATTCTCTGTTGCATCTGAAAACAGTCAAACATGAGCTTCTTC CAAGTGTGAATGACATCACAGCTGTTGGACCGGCACATTTCTATGCCACAAATGACCACTACTTCTCTGA TCCTTTCTTAAAGTATTTAGAAACATACTTGAACTTACACTGGGCAAATGTTGTTTACTACAGTCCAAAT GAAGTTAAAGTGGTAGCAGAAGGATTTGATTCAGCAAATGGGATCAATATTTCACCTGATGATAAGTATA TCTATGTTGCTGACATATTGGCTCATGAAATTCATGTTTTGGAAAAACACACTAATATGAATTTAACTCA GTTGAAGGTACTTGAGCTGGATACACTGGTGGATAATTTATCTATTGATCCTTCCTCGGGGGACATCTGG GTAGGCTGTCATCCTAATGGCCAGAAGCTCTTCGTGTATGACCCGAACAATCCTCCCTCGTCAGAGGTTC TCCGCATCCAGAACATTCTATCTGAGAAGCCTACAGTGACTACAGTTTATGCCAACAATGGGTCTGTTCT CCAAGGAAGTTCTGTAGCCTCAGTGTATGATGGGAAGCTGCTCATAGGCACTTTATACCACAGAGCCTTG TATTGTGAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222624 representing NM_000305
Red=Cloning site Green=Tags(s) MGRLVAVGLLGIALALLGERLLALRNRLKASREVESVDLPHCHLIKGIEAGSEDIDILPNGLAFFSVGLK FPGLHSFAPDKPGGILMMDLKEEKPRARELRISRGFDLASFNPHGISTFIDNDDTVYLFVVNHPEFKNTV EIFKFEEAENSLLHLKTVKHELLPSVNDITAVGPAHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPN EVKVVAEGFDSANGINISPDDKYIYVADILAHEIHVLEKHTNMNLTQLKVLELDTLVDNLSIDPSSGDIW VGCHPNGQKLFVYDPNNPPSSEVLRIQNILSEKPTVTTVYANNGSVLQGSSVASVYDGKLLIGTLYHRAL YCEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000305 |
| ORF Size | 1062 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000305.3 |
| RefSeq Size | 1669 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 5445 |
| UniProt ID | Q15165 |
| Domains | Arylesterase |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways |
| MW | 39.2 kDa |
| Gene Summary | This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222624L1 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222624L2 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC222624L3 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC222624L4 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG222624 | PON2 (tGFP-tagged) - Human paraoxonase 2 (PON2), transcript variant 1 |
CNY 5,256.00 |
|
| SC119958 | PON2 (untagged)-Human paraoxonase 2 (PON2), transcript variant 1 |
CNY 3,656.00 |
