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NOS1 (NM_000620) Human Tagged ORF Clone
CAT＃: RC222384
NOS1 (Myc-DDK-tagged)-Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1
ORF Plasmid: tGFP
"NM_000620" in other vectors (6)
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CNY 9,816.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222384 representing NM_000620
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATCACATGTTCGGTGTTCAGCAAATCCAGCCCAATGTCATTTCTGTTCGTCTCTTCAAGCGCA AAGTTGGGGGCCTGGGATTTCTGGTGAAGGAGCGGGTCAGTAAGCCGCCCGTGATCATCTCTGACCTGAT TCGTGGGGGCGCCGCAGAGCAGAGTGGCCTCATCCAGGCCGGAGACATCATTCTTGCGGTCAACGGCCGG CCCTTGGTGGACCTGAGCTATGACAGCGCCCTGGAGGTACTCAGAGGCATTGCCTCTGAGACCCACGTGG TCCTCATTCTGAGGGGCCCTGAAGGTTTCACCACGCACCTGGAGACCACCTTTACAGGTGATGGGACCCC CAAGACCATCCGGGTGACACAGCCCCTGGGTCCCCCCACCAAAGCCGTGGATCTGTCCCACCAGCCACCG GCCGGCAAAGAACAGCCCCTGGCAGTGGATGGGGCCTCGGGTCCCGGGAATGGGCCTCAGCATGCCTACG ATGATGGGCAGGAGGCTGGCTCACTCCCCCATGCCAACGGCCTGGCCCCCAGGCCCCCAGGCCAGGACCC CGCGAAGAAAGCAACCAGAGTCAGCCTCCAAGGCAGAGGGGAGAACAATGAACTGCTCAAGGAGATAGAG CCTGTGCTGAGCCTTCTCACCAGTGGGAGCAGAGGGGTCAAGGGAGGGGCACCTGCCAAGGCAGAGATGA AAGATATGGGAATCCAGGTGGACAGAGATTTGGACGGCAAGTCACACAAACCTCTGCCCCTCGGCGTGGA GAACGACCGAGTCTTCAATGACCTATGGGGGAAGGGCAATGTGCCTGTCGTCCTCAACAACCCATATTCA GAGAAGGAGCAGCCCCCCACCTCAGGAAAACAGTCCCCCACAAAGAATGGCAGCCCCTCCAAGTGTCCAC GCTTCCTCAAGGTCAAGAACTGGGAGACTGAGGTGGTTCTCACTGACACCCTCCACCTTAAGAGCACATT GGAAACGGGATGCACTGAGTACATCTGCATGGGCTCCATCATGCATCCTTCTCAGCATGCAAGGAGGCCT GAAGACGTCCGCACAAAAGGACAGCTCTTCCCTCTCGCCAAAGAGTTTATTGATCAATACTATTCATCAA TTAAAAGATTTGGCTCCAAAGCCCACATGGAAAGGCTGGAAGAGGTGAACAAAGAGATCGACACCACTAG CACTTACCAGCTCAAGGACACAGAGCTCATCTATGGGGCCAAGCACGCCTGGCGGAATGCCTCGCGCTGT GTGGGCAGGATCCAGTGGTCCAAGCTGCAGGTATTCGATGCCCGTGACTGCACCACGGCCCACGGGATGT TCAACTACATCTGTAACCATGTCAAGTATGCCACCAACAAAGGGAACCTCAGGTCTGCCATCACCATATT CCCCCAGAGGACAGACGGCAAGCACGACTTCCGAGTCTGGAACTCCCAGCTCATCCGCTACGCTGGCTAC AAGCAGCCTGACGGCTCCACCCTGGGGGACCCAGCCAATGTGCAGTTCACAGAGATATGCATACAGCAGG GCTGGAAACCGCCTAGAGGCCGCTTCGATGTCCTGCCGCTCCTGCTTCAGGCCAACGGCAATGACCCTGA GCTCTTCCAGATTCCTCCAGAGCTGGTGTTGGAAGTTCCCATCAGGCACCCCAAGTTTGAGTGGTTCAAG GACCTGGGGCTGAAGTGGTACGGCCTCCCCGCCGTGTCCAACATGCTCCTAGAGATTGGCGGCCTGGAGT TCAGCGCCTGTCCCTTCAGTGGCTGGTACATGGGCACAGAGATTGGTGTCCGCGACTACTGTGACAACTC CCGCTACAATATCCTGGAGGAAGTGGCCAAGAAGATGAACTTAGACATGAGGAAGACGTCCTCCCTGTGG AAGGACCAGGCGCTGGTGGAGATCAATATCGCGGTTCTCTATAGCTTCCAGAGTGACAAAGTGACCATTG TTGACCATCACTCCGCCACCGAGTCCTTCATTAAGCACATGGAGAATGAGTACCGCTGCCGGGGGGGCTG CCCTGCCGACTGGGTGTGGATCGTGCCCCCCATGTCCGGAAGCATCACCCCTGTGTTCCACCAGGAGATG CTCAACTACCGGCTCACCCCCTCCTTCGAATACCAGCCTGATCCCTGGAACACGCATGTCTGGAAAGGCA CCAACGGGACCCCCACAAAGCGGCGAGCCATCGGCTTCAAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGC CAAGCTGATGGGGCAGGCTATGGCCAAGAGGGTGAAAGCGACCATCCTCTATGCCACAGAGACAGGCAAA TCGCAAGCTTATGCCAAGACCTTGTGTGAGATCTTCAAACACGCCTTTGATGCCAAGGTGATGTCCATGG AAGAATATGACATTGTGCACCTGGAACATGAAACTCTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGG AGATCCCCCTGAGAATGGGGAGAAATTCGGCTGTGCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAG GAAGAAAGGAAGAGCTACAAGGTCCGATTCAACAGCGTCTCCTCCTACTCTGACTCCCAAAAATCATCAG GCGATGGGCCCGACCTCAGAGACAACTTTGAGAGTGCTGGACCCCTGGCCAATGTGAGGTTCTCAGTTTT TGGCCTCGGCTCACGAGCATACCCTCACTTTTGCGCCTTCGGACACGCTGTGGACACCCTCCTGGAAGAA CTGGGAGGGGAGAGGATCTTGAAGATGAGGGAAGGGGATGAGCTCTGTGGGCAGGAAGAGGCTTTCAGGA CCTGGGCCAAGAAGGTCTTCAAGGCAGCCTGTGATGTCTTCTGTGTGGGAGATGATGTCAACATTGAAAA GGCCAACAATTCCCTCATCAGCAATGATCGCAGCTGGAAGAGAAACAAGTTCCGCCTCACCTTTGTGGCC GAAGCTCCAGAACTCACACAAGGTCTATCCAATGTCCACAAAAAGCGAGTCTCAGCTGCCCGGCTCCTTA GCCGTCAAAACCTCCAGAGCCCTAAATCCAGTCGGTCAACTATCTTCGTGCGTCTCCACACCAACGGGAG CCAGGAGCTGCAGTACCAGCCTGGGGACCACCTGGGTGTCTTCCCTGGCAACCACGAGGACCTCGTGAAT GCCCTGATCGAGCGGCTGGAGGACGCGCCGCCTGTCAACCAGATGGTGAAAGTGGAACTGCTGGAGGAGC GGAACACGGCTTTAGGTGTCATCAGTAACTGGACAGACGAGCTCCGCCTCCCGCCCTGCACCATCTTCCA GGCCTTCAAGTACTACCTGGACATCACCACGCCACCAACGCCTCTGCAGCTGCAGCAGTTTGCCTCCCTA GCTACCAGCGAGAAGGAGAAGCAGCGTCTGCTGGTCCTCAGCAAGGGTTTGCAGGAGTACGAGGAATGGA AATGGGGCAAGAACCCCACCATCGTGGAGGTGCTGGAGGAGTTCCCATCTATCCAGATGCCGGCCACCCT GCTCCTGACCCAGCTGTCCCTGCTGCAGCCCCGCTACTATTCCATCAGCTCCTCCCCAGACATGTACCCT GATGAAGTGCACCTCACTGTGGCCATCGTTTCCTACCGCACTCGAGATGGAGAAGGACCAATTCACCACG GCGTATGCTCCTCCTGGCTCAACCGGATACAGGCTGACGAACTGGTCCCCTGTTTCGTGAGAGGAGCACC CAGCTTCCACCTGCCCCGGAACCCCCAAGTCCCCTGCATCCTCGTTGGACCAGGCACCGGCATTGCCCCT TTCCGAAGCTTCTGGCAACAGCGGCAATTTGATATCCAACACAAAGGAATGAACCCCTGCCCCATGGTCC TGGTCTTCGGGTGCCGGCAATCCAAGATAGATCATATCTACAGGGAAGAGACCCTGCAGGCCAAGAACAA GGGGGTCTTCAGAGAGCTGTACACGGCTTACTCCCGGGAGCCAGACAAACCAAAGAAGTACGTGCAGGAC ATCCTGCAGGAGCAGCTGGCGGAGTCTGTGTACCGAGCCCTGAAGGAGCAAGGGGGCCACATATACGTCT GTGGGGACGTCACCATGGCTGCTGATGTCCTCAAAGCCATCCAGCGCATCATGACCCAGCAGGGGAAGCT CTCGGCAGAGGACGCCGGCGTATTCATCAGCCGGATGAGGGATGACAACCGATACCATGAGGATATTTTT GGAGTCACCCTGCGAACGTACGAAGTGACCAACCGCCTTAGATCTGAGTCCATTGCCTTCATTGAAGAGA GCAAAAAAGACACCGATGAGGTTTTCAGCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222384 representing NM_000620
Red=Cloning site Green=Tags(s) MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGR PLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPP AGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIE PVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYS EKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRP EDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRC VGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGY KQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFK DLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLW KDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEM LNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGK SQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQ EERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEE LGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVA EAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVN ALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASL ATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYP DEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAP FRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQD ILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIF GVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000620 |
| ORF Size | 4302 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000620.5 |
| RefSeq Size | 7124 bp |
| RefSeq ORF | 4305 bp |
| Locus ID | 4842 |
| UniProt ID | P29475 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer |
| MW | 160.8 kDa |
| Gene Summary | The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
NOS induction and NOSIP interaction impact granulopoiesis and neutrophil differentiation by modulating nitric oxide generation
,Sadaf, S;Nagarkoti, S;Awasthi, D;Singh, AK;Srivastava, RN;Kumar, S;Barthwal, MK;Dikshit, M;,
Biochimica et biophysica acta. Molecular cell research
,PubMed ID 33771575
[NOS1]
|
|
Pyroptotic and apoptotic cell death in iNOS and nNOS overexpressing K562 cells: a mechanistic insight
,Sadaf, S;Awasthi, D;Singh, AK;Nagarkoti, S;Kumar, S;Barthwal, MK;Dikshit, M;,
Biochem. Pharmacol.
,PubMed ID 31881190
[NOS1]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222384L1 | Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, Myc-DDK-tagged |
CNY 12,216.00 |
|
| RC222384L2 | Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, mGFP tagged |
CNY 16,250.00 |
|
| RC222384L3 | Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, Myc-DDK-tagged |
CNY 12,216.00 |
|
| RC222384L4 | Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, mGFP tagged |
CNY 12,216.00 |
|
| RG222384 | NOS1 (tGFP-tagged) - Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 |
CNY 11,416.00 |
|
| SC300110 | NOS1 (untagged)-Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 |
CNY 9,816.00 |
