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PDI (PDIA2) (NM_006849) Human Tagged ORF Clone
CAT＃: RC222382
PDIA2 (Myc-DDK-tagged)-Human protein disulfide isomerase family A, member 2 (PDIA2)
ORF Plasmid: tGFP
"NM_006849" in other vectors (7)
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CNY 3,912.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PDA2; PDI; PDIP; PDIR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222382 representing NM_006849
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCCAGCTTCTGCCTGTACTGCTGCTGCTGCTGCTCAGGGCTTCGTGCCCATGGGGTCAGGAAC AGGGAGCGAGGAGCCCCTCGGAGGAGCCTCCAGAGGAGGAAATCCCCAAGGAGGATGGGATCTTGGTGCT GAGCCGCCACACCCTGGGCCTGGCCCTGCGGGAGCACCCTGCCCTGCTGGTGGAATTCTATGCCCCGTGG TGTGGGCACTGCCAGGCCCTGGCCCCCGAGTACAGCAAGGCAGCTGCCGTGCTCGCGGCCGAGTCAATGG TGGTCACGCTGGCCAAGGTGGATGGGCCCGCGCAGCGCGAGCTGGCTGAGGAGTTTGGTGTGACGGAGTA CCCTACGCTCAAGTTCTTCCGCAATGGGAACCGCACGCACCCGGAGGAGTACACAGGACCACGGGACGCT GAGGGCATTGCCGAGTGGCTGCGACGGCGGGTGGGGCCCAGTGCCATGCGGCTGGAGGACGAGGCGGCCG CCCAGGCGCTGATCGGTGGCCGGGACCTAGTGGTCATTGGCTTCTTCCAGGACCTGCAGGACGAGGACGT GGCCACCTTCTTGGCCTTGGCCCAGGACGCCCTGGACATGACCTTTGGCCTCACAGACCGGCCGCGGCTC TTTCAGCAGTTTGGCCTCACCAAGGACACTGTGGTTCTCTTCAAGAAGTTTGATGAGGGGCGGGCAGACT TCCCCGTGGACGAGGAGCTTGGCCTGGACCTGGGGGATCTGTCGCGCTTCCTGGTCACACACAGCATGCG CCTGGTCACGGAGTTCAACAGCCAGACGTCTGCCAAGATCTTCGCGGCCAGGATCCTCAACCACCTGCTG CTGTTTGTCAACCAGACGCTGGCTGCGCACCGGGAGCTCCTAGCGGGCTTTGGGGAGGCAGCTCCCCGCT TCCGGGGGCAGGTGCTGTTCGTGGTGGTGGACGTGGCGGCCGACAATGAGCACGTGCTGCAGTACTTTGG ACTCAAGGCTGAGGCAGCCCCCACTCTGCGCTTGGTCAACCTTGAAACCACTAAGAAGTATGCGCCTGTG GATGGGGGCCCTGTCACCGCAGCGTCCATCACTGCTTTCTGCCATGCAGTCCTCAACGGCCAAGTCAAGC CCTATCTCCTGAGCCAGGAGATACCCCCTGATTGGGATCAGCGGCCAGTTAAGACCCTCGTGGGCAAGAA TTTTGAGCAGGTGGCTTTTGACGAAACCAAGAATGTGTTTGTCAAGTTCTATGCCCCGTGGTGCACCCAC TGCAAGGAGATGGCCCCTGCCTGGGAGGCATTGGCTGAGAAGTACCAAGACCACGAGGACATCATCATTG CTGAGCTGGATGCCACGGCCAACGAGCTGGATGCCTTCGCTGTGCACGGCTTCCCTACTCTCAAGTACTT CCCAGCAGGGCCAGGTCGGAAGGTGATTGAATACAAAAGCACCAGGGACCTGGAGACTTTCTCCAAGTTC CTGGACAACGGGGGCGTGCTGCCCACGGAGGAGCCCCCGGAGGAGCCAGCAGCCCCGTTCCCGGAGCCAC CGGCCAACTCCACTATGGGGTCCAAGGAGGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222382 representing NM_006849
Red=Cloning site Green=Tags(s) MSRQLLPVLLLLLLRASCPWGQEQGARSPSEEPPEEEIPKEDGILVLSRHTLGLALREHPALLVEFYAPW CGHCQALAPEYSKAAAVLAAESMVVTLAKVDGPAQRELAEEFGVTEYPTLKFFRNGNRTHPEEYTGPRDA EGIAEWLRRRVGPSAMRLEDEAAAQALIGGRDLVVIGFFQDLQDEDVATFLALAQDALDMTFGLTDRPRL FQQFGLTKDTVVLFKKFDEGRADFPVDEELGLDLGDLSRFLVTHSMRLVTEFNSQTSAKIFAARILNHLL LFVNQTLAAHRELLAGFGEAAPRFRGQVLFVVVDVAADNEHVLQYFGLKAEAAPTLRLVNLETTKKYAPV DGGPVTAASITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKTLVGKNFEQVAFDETKNVFVKFYAPWCTH CKEMAPAWEALAEKYQDHEDIIIAELDATANELDAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKF LDNGGVLPTEEPPEEPAAPFPEPPANSTMGSKEEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006849 |
| ORF Size | 1575 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006849.4 |
| RefSeq Size | 1726 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 64714 |
| UniProt ID | Q13087 |
| Protein Families | Druggable Genome |
| MW | 58 kDa |
| Gene Summary | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels. [provided by RefSeq, Sep 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Integrated cell and process engineering for improved transient production of a difficult-to-express fusion protein by CHO cells
,Johari, YB;Estes, SD;Alves, CS;Sinacore, MS;James, DC;,
Biotechnol. Bioeng.
,PubMed ID 26126657
[PDI]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222382L1 | Lenti ORF clone of Human protein disulfide isomerase family A, member 2 (PDIA2), Myc-DDK-tagged |
CNY 6,312.00 |
|
| RC222382L2 | Lenti ORF clone of Human protein disulfide isomerase family A, member 2 (PDIA2), mGFP tagged |
CNY 6,460.00 |
|
| RC222382L3 | Lenti ORF clone of Human protein disulfide isomerase family A, member 2 (PDIA2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC222382L4 | Lenti ORF clone of Human protein disulfide isomerase family A, member 2 (PDIA2), mGFP tagged |
CNY 6,460.00 |
|
| RG222382 | PDIA2 (tGFP-tagged) - Human protein disulfide isomerase family A, member 2 (PDIA2) |
CNY 5,512.00 |
|
| SC127860 | PDIA2 (untagged)-Human protein disulfide isomerase family A, member 2 (PDIA2) |
CNY 4,312.00 |
|
| SC317891 | PDIA2 (untagged)-Human protein disulfide isomerase family A, member 2 (PDIA2) |
CNY 3,920.00 |
