Flt3 ligand (FLT3LG) (NM_001459) Human Tagged ORF Clone
CAT#: RC222242
FLT3LG (Myc-DDK-tagged)-Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3
ORF Plasmid: tGFP
"NM_001459" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
CNY 3,990.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FL; FLG3L; FLT3L |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222242 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGTGCTGGCGCCAGCCTGGAGCCCAACGACCTATCTCCTCCTGCTGCTGCTGCTGAGCTCGGGAC TCAGTGGGACCCAGGACTGCTCCTTCCAACACAGCCCCATCTCCTCCGACTTCGCTGTCAAAATCCGTGA GCTGTCTGACTACCTGCTTCAAGATTACCCAGTCACCGTGGCCTCCAACCTGCAGGACGAGGAGCTCTGC GGGGGCCTCTGGCGGCTGGTCCTGGCACAGCGCTGGATGGAGCGGCTCAAGACTGTCGCTGGGTCCAAGA TGCAAGGCTTGCTGGAGCGCGTGAACACGGAGATACACTTTGTCACCAAATGTGCCTTTCAGCCCCCCCC CAGCTGTCTTCGCTTCGTCCAGACCAACATCTCCCGCCTCCTGCAGGAGACCTCCGAGCAGCTGGTGGCG CTGAAGCCCTGGATCACTCGCCAGAACTTCTCCCGGTGCCTGGAGCTGCAGTGTCAGCCCGACTCCTCAA CCCTGCCACCCCCATGGAGTCCCCGGCCCCTGGAGGCCACAGCCCCGACAGCCCCGCAGCCCCCTCTGCT CCTCCTACTGCTGCTGCCCGTGGGCCTCCTGCTGCTGGCCGCTGCCTGGTGCCTGCACTGGCAGAGGACG CGGCGGAGGACACCCCGCCCTGGGGAGCAGGTGCCCCCCGTCCCCAGTCCCCAGGACCTGCTGCTTGTGG AGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222242 protein sequence
Red=Cloning site Green=Tags(s) MTVLAPAWSPTTYLLLLLLLSSGLSGTQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELC GGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVA LKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTAPQPPLLLLLLLPVGLLLLAAAWCLHWQRT RRRTPRPGEQVPPVPSPQDLLLVEH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001459 |
ORF Size | 705 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001459.4 |
RefSeq Size | 1074 bp |
RefSeq ORF | 708 bp |
Locus ID | 2323 |
UniProt ID | P49771 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer |
MW | 26.4 kDa |
Gene Summary | Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Engineered niches support the development of human dendritic cells in humanized mice
,Anselmi, G;Vaivode, K;Dutertre, CA;Bourdely, P;,
bioRxiv
[Flt3 ligand]
|
A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis
,Li, C;Wang, S;Xing, Z;Lin, A;Liang, K;Song, J;Hu, Q;Yao, J;Chen, Z;Park, PK;Hawke, DH;Zhou, J;Zhou, Y;Zhang, S;Liang, H;Hung, MC;Gallick, GE;Han, L;Lin, C;Yang, L;,
Nat. Cell Biol
,PubMed ID 28114269
[Flt3 ligand]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222242L1 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC222242L2 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RC222242L3 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC222242L4 | Lenti ORF clone of Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, mGFP tagged |
CNY 4,800.00 |
|
RG222242 | FLT3LG (tGFP-tagged) - Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3 |
CNY 4,000.00 |
|
SC303027 | FLT3LG (untagged)-Human fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3 |
CNY 2,400.00 |
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