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PI 3 Kinase Class 2A (PIK3C2A) (NM_002645) Human Tagged ORF Clone
CAT＃: RC222192
- TrueORF®
PIK3C2A (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A)
ORF Plasmid: tGFP
"NM_002645" in other vectors (4)
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CNY 11,336.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CPK; OCSKD; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222192 representing NM_002645
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGATATCTAGCAACAGCGGATTTAAAGAATGTCCATCTTCACATCCGGAACCAACAAGAGCAA AAGATGTGGACAAAGAAGAAGCATTACAGATGGAAGCAGAGGCTTTAGCAAAACTGCAAAAGGATAGACA AGTGACTGACAATCAGAGAGGCTTTGAGTTGTCAAGCAGCACCAGAAAAAAAGCACAGGTTTATAACAAG CAGGATTATGATCTCATGGTGTTTCCTGAATCAGATTCCCAAAAAAGAGCATTAGATATTGATGTAGAAA AGCTCACCCAAGCTGAACTTGAGAAACTATTGCTGGATGACAGTTTCGAGACTAAAAAAACACCTGTATT ACCAGTTACTCCTATTCTGAGCCCTTCCTTTTCAGCACAGCTCTATTTTAGACCTACTATTCAGAGAGGA CAGTGGCCACCTGGATTACCTGGGCCTTCCACTTATGCTTTACCTTCTATTTATCCTTCTACTTACAGTA AACAGGCTGCATTCCAAAATGGCTTCAATCCAAGAATGCCCACTTTTCCATCTACAGAACCTATATATTT AAGTCTTCCGGGACAATCTCCATATTTCTCATATCCTTTGACACCTGCCACACCCTTTCATCCACAAGGA AGCTTACCTATCTATCGTCCAGTAGTCAGTACTGACATGGCAAAACTATTTGACAAAATAGCTAGTACAT CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAACAGATTCAAAAGTCAGCAATCTACA GGTATCTCCAAAGTCTGAGGATATCAGTAAATTTGACTGGTTAGACTTGGATCCTCTAAGTAAGCCTAAG GTGGATAATGTGGAGGTATTAGACCATGAGGAAGAGAAAAATGTTTCAAGTTTGCTAGCAAAGGATCCTT GGGATGCTGTTCTTCTTGAAGAGAGATCGACAGCAAATTGTCATCTTGAAAGAAAGGTGAATGGAAAATC CCTTTCTGTGGCAACTGTTACAAGAAGCCAGTCTTTAAATATTCGAACAACTCAGCTTGCAAAAGCCCAG GGCCATATATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTTCAAGAAG TTGAAGTACAGAATGAGGAGATGGCAGCTTTTTGTCGATCCATTACAAAATTGAAGACCAAATTTCCATA TACCAATCACCGCACAAACCCAGGCTATTTGTTAAGTCCAGTCACAGCGCAAAGAAACATATGCGGAGAA AATGCTAGTGTGAAGGTCTCCATTGACATTGAAGGATTTCAGCTACCAGTTACTTTTACGTGTGATGTGA GTTCTACTGTAGAAATCATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGTAGATGT TGGCAGCTATGTTCTAAAAGTTTGTGGTCAAGAGGAAGTGCTGCAGAATAATCATTGCCTTGGAAGTCAT GAGCATATTCAAAACTGTCGAAAATGGGACACAGAAATTAGACTACAACTCTTGACCTTCAGTGCAATGT GTCAAAATCTGGCCCGAACAGCAGAAGATGATGAAACACCCGTGGATTTAAACAAACACCTGTATCAAAT AGAAAAACCTTGCAAAGAAGCCATGACGAGACACCCTGTTGAAGAACTCTTAGATTCTTATCACAACCAA GTAGAACTGGCTCTTCAAATTGAAAACCAACACCGAGCAGTAGATCAAGTAATTAAAGCTGTAAGAAAAA TCTGTAGTGCTTTAGATGGTGTCGAGACTCTTGCCATTACAGAATCAGTAAAGAAGCTAAAGAGAGCAGT TAATCTTCCAAGGAGTAAAACTGCTGATGTGACTTCTTTGTTTGGAGGAGAAGACACTAGCAGGAGTTCA ACTAGGGGCTCACTTAATCCTGAAAATCCTGTTCAAGTAAGCATAAACCAATTAACTGCAGCAATTTATG ATCTTCTCAGACTCCATGCAAATTCTGGTAGGAGTCCTACAGACTGTGCCCAAAGTAGCAAGAGTGTCAA GGAAGCATGGACTACAACAGAGCAGCTCCAGTTTACTATTTTTGCTGCTCATGGAATTTCAAGTAATTGG GTATCAAATTATGAAAAATACTACTTGATATGTTCACTGTCTCACAATGGAAAGGATCTTTTTAAACCTA TTCAATCAAAGAAGGTTGGCACTTACAAGAATTTCTTCTATCTTATTAAATGGGATGAACTAATCATTTT TCCTATCCAGATATCACAATTGCCATTAGAATCAGTTCTTCACCTTACTCTTTTTGGAATTTTAAATCAG AGCAGTGGAAGTTCCCCTGATTCTAATAAGCAGAGAAAGGGACCAGAAGCTTTGGGCAAAGTTTCTTTAC CTCTTTTTGACTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTATATCTTTGGACTTCATCACATAC AAATTCTGTTCCTGGAACAGTTACCAAAAAAGGATATGTCATGGAAAGAATAGTGCTACAGGTTGATTTT CCTTCTCCTGCATTTGATATTATTTATACAACTCCTCAAGTTGACAGAAGCATTATACAGCAACATAACT TAGAAACACTAGAGAATGATATAAAAGGGAAACTTCTTGATATTCTTCATAAAGACTCATCACTTGGACT TTCTAAAGAAGATAAAGCTTTTTTATGGGAGAAACGTTATTATTGCTTCAAACACCCAAATTGTCTTCCT AAAATATTAGCAAGCGCCCCAAACTGGAAATGGGTTAATCTTGCCAAAACTTACTCATTGCTTCACCAGT GGCCTGCATTGTACCCACTAATTGCATTGGAACTTCTTGATTCAAAATTTGCTGATCAGGAAGTAAGATC CCTAGCTGTGACCTGGATTGAGGCCATTAGTGATGATGAGCTAACAGATCTTCTTCCACAGTTTGTACAA GCTTTGAAATATGAAATTTACTTGAATAGTTCATTAGTGCAATTCCTTTTGTCCAGGGCATTGGGAAATA TCCAGATAGCACACAATTTATATTGGCTTCTCAAAGATGCCCTGCATGATGTACAGTTTAGTACCCGATA CGAACATGTTTTGGGTGCTCTCCTGTCAGTAGGAGGAAAACGACTTAGAGAAGAACTTCTAAAACAGACG AAACTTGTACAGCTTTTAGGAGGAGTAGCAGAAAAAGTAAGGCAGGCTAGTGGATCAGCCAGACAGGTTG TTCTCCAAAGAAGTATGGAACGAGTACAGTCCTTTTTTCAGAAAAATAAATGCCGTCTCCCTCTCAAGCC AAGTCTAGTGGCAAAAGAATTAAATATTAAGTCGTGTTCCTTCTTCAGTTCTAATGCTGTCCCCCTAAAA GTCACAATGGTGAATGCTGACCCTATGGGAGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATCTTC GGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAAGGACTAGATCT GAGGATGGTAATTTTCAAATGTCTCTCAACTGGCAGAGATCGAGGCATGGTGGAGCTGGTTCCTGCTTCC GATACCCTCAGGAAAATCCAAGTGGAATATGGTGTGACAGGATCCTTTAAAGATAAACCACTTGCAGAGT GGCTAAGGAAATACAATCCCTCTGAAGAAGAATATGAAAAGGCTTCAGAGAACTTTATCTATTCCTGTGC TGGATGCTGTGTAGCCACCTATGTTTTAGGCATCTGTGATCGACACAATGACAATATAATGCTTCGAAGC ACGGGACACATGTTTCACATTGACTTTGGAAAGTTTTTGGGACATGCACAGATGTTTGGCAGCTTCAAAA GGGATCGGGCTCCTTTTGTGCTGACCTCTGATATGGCATATGTCATTAATGGGGGTGAAAAGCCCACCAT TCGTTTTCAGTTGTTTGTGGACCTCTGCTGTCAGGCCTACAACTTGATAAGAAAGCAGACAAACCTTTTT CTTAACCTCCTTTCACTGATGATTCCTTCAGGGTTACCAGAACTTACAAGTATTCAAGATTTGAAATACG TTAGAGATGCACTTCAACCCCAAACTACAGACGCAGAAGCTACAATTTTCTTTACTAGGCTTATTGAATC AAGTTTGGGAAGCATTGCCACAAAGTTTAACTTCTTCATTCACAACCTTGCTCAGCTTCGTTTTTCTGGT CTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTCCTTTAGACAAGATGGTCGAA TCAAGGAAGTCTCTGTTTTTACATATCATAAGAAATACAACCCAGATAAACATTATATTTATGTAGTCCG AATTTTGAGGGAAGGACAGATTGAACCATCATTTGTCTTCCGAACATTTGACGAATTTCAGGAACTTCAC AATAAGCTCAGTATTATTTTTCCACTTTGGAAGTTACCAGGCTTTCCTAATAGGATGGTTCTAGGAAGAA CACACATAAAAGATGTAGCAGCCAAAAGGAAAATTGAGTTAAACAGTTACTTACAGAGTTTGATGAATGC TTCAACGGATGTAGCAGAGTGTGATCTTGTTTGTACTTTCTTCCACCCTTTACTTCGTGATGAGAAAGCT GAAGGGATAGCTAGGTCTGCAGATGCAGGTTCCTTCAGTCCTACTCCAGGCCAAATAGGAGGAGCTGTGA AATTATCCATCTCTTACCGAAATGGTACTCTTTTCATCATGGTGATGCATATCAAAGATCTTGTTACTGA AGATGGAGCTGACCCAAATCCATATGTCAAAACATACCTACTTCCAGATAACCACAAAACATCCAAACGT AAAACCAAAATTTCACGAAAAACGAGGAATCCGACATTCAATGAAATGCTTGTATACAGTGGATATAGCA AAGAAACCCTAAGACAGCGAGAACTTCAACTAAGTGTACTCAGTGCAGAATCTCTGCGGGAGAATTTTTT CTTGGGTGGAGTAACCCTGCCTTTGAAAGATTTCAACTTGAGCAAAGAGACGGTTAAATGGTATCAGCTG ACTGCGGCAACATACTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222192 representing NM_002645
Red=Cloning site Green=Tags(s) MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNK QDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRG QWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQG SLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPK VDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQSLNIRTTQLAKAQ GHISQKDPNGTSSLPTGSSLLQEVEVQNEEMAAFCRSITKLKTKFPYTNHRTNPGYLLSPVTAQRNICGE NASVKVSIDIEGFQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQNNHCLGSH EHIQNCRKWDTEIRLQLLTFSAMCQNLARTAEDDETPVDLNKHLYQIEKPCKEAMTRHPVEELLDSYHNQ VELALQIENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAVNLPRSKTADVTSLFGGEDTSRSS TRGSLNPENPVQVSINQLTAAIYDLLRLHANSGRSPTDCAQSSKSVKEAWTTTEQLQFTIFAAHGISSNW VSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGILNQ SSGSSPDSNKQRKGPEALGKVSLPLFDFKRFLTCGTKLLYLWTSSHTNSVPGTVTKKGYVMERIVLQVDF PSPAFDIIYTTPQVDRSIIQQHNLETLENDIKGKLLDILHKDSSLGLSKEDKAFLWEKRYYCFKHPNCLP KILASAPNWKWVNLAKTYSLLHQWPALYPLIALELLDSKFADQEVRSLAVTWIEAISDDELTDLLPQFVQ ALKYEIYLNSSLVQFLLSRALGNIQIAHNLYWLLKDALHDVQFSTRYEHVLGALLSVGGKRLREELLKQT KLVQLLGGVAEKVRQASGSARQVVLQRSMERVQSFFQKNKCRLPLKPSLVAKELNIKSCSFFSSNAVPLK VTMVNADPMGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDRGMVELVPAS DTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRS TGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLF LNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSG LPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGQIEPSFVFRTFDEFQELH NKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKA EGIARSADAGSFSPTPGQIGGAVKLSISYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDNHKTSKR KTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGVTLPLKDFNLSKETVKWYQL TAATYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002645 |
| ORF Size | 5058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002645.4 |
| RefSeq Size | 5061 bp |
| RefSeq ORF | 5061 bp |
| Locus ID | 5286 |
| UniProt ID | O00443 |
| Domains | C2, PI3K_rbd, PI3_PI4_kinase, PI3Ka, PX, PI3K_C2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| MW | 190.5 kDa |
| Gene Summary | The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222192L1 | Lenti ORF clone of Human phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A), Myc-DDK-tagged |
CNY 13,736.00 |
|
| RC222192L3 | Lenti ORF clone of Human phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A), Myc-DDK-tagged |
CNY 18,720.00 |
|
| RG222192 | PIK3C2A (tGFP-tagged) - Human phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A) |
CNY 12,936.00 |
|
| SC309022 | PIK3C2A (untagged)-Human phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A) |
CNY 11,344.00 |
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