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PIMT (TGS1) (NM_024831) Human Tagged ORF Clone
CAT＃: RC221904
- TrueORF®
TGS1 (Myc-DDK-tagged)-Human trimethylguanosine synthase 1 (TGS1)
ORF Plasmid: tGFP
"NM_024831" in other vectors (4)
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CNY 6,248.00
CNY 8,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NCOA6IP; PIMT; PIPMT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221904 representing NM_024831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTGCGAGAAGTGGAGCCGCGTGGCGGAAATGTTTCTCTTCATTGAGGAGCGGGAGGATTGTAAGA TACTGTGCCTTTGCTCCAGGGCATTTGTGGAGGATCGAAAATTGTACAATTTGGGATTAAAAGGCTATTA CATCAGAGACAGTGGCAACAATTCAGGAGACCAGGCGACAGAAGAAGAGGAAGGTGGTTATTCCTGTGGT ACTGCAGAATCACATGACAGCAAAGGCATAGGCCTGGATGAAAGTGAACTTGATTCTGAGGCTGAACTCA TGAGAAGTATGGGATTGCCACTTCAATTTGGTAGGATAACTGCACATAAGGATTTTGAGGTATCTATGAA TACTAGAAATAAAGTTAAAATAAAAAAGAAAAAACATCAAAAGAAATACTTAGATGAAATTGTGCAAGAA TCTTGGAGAAAAGAATATGAAGAAGACGACATTTTGGCTTCAGATGATCCATCTTCAATTGAACAGTATG AGAACACCAGAACATATGAACTTCAAAGCAAAAAAGATACTGAGACAGAAAATCCTCCAGTTGAAAACAC ATTATCTCCAAAGCTAGAAATTACAGAGAAATGGGAAAAGTATTGGAATGAATATGGAGGAGGACTATTG TGGCAAAGTTGGCAAGAAAAACATCCGGGTCAAGCACTATCTTCTGAACCTTGGAACTTTCCTGATACAA AGGAAGAATGGGAGCAACATTATAGTCAACTTTATTGGTATTATTTGGAACAATTTCAGTATTGGGAAGC TCAGGGTTGGACTTTTGATGCCTCGCAAAGCTGTGATACAGATACTTACACATCTAAAACAGAAGCTGAT GACAAGAACGATGAAAAATGCATGAAAGTTGACTTAGTATCTTTTCCATCTTCACCTATTATGGTTGATA ATGATAGCTCTGGTACAAGTGATAAGGATCATAGTGAAATACTTGATGGAATTAGTAACATAAAACTGAA TTCAGAGGAAGTAACACAGAGCCAATTAGATTCCTGTACAAGTCATGATGGTCATCAACAGCTAAGTGAA GTTAGTAGCAAAAGAGAGTGCCCTGCTTCCGGCCAAAGTGAACCACGTAATGGAGGAACCAATGAGGAAA GCAACTCATCGGGGAATACAAACACAGACCCACCAGCTGAGGATTCACAGAAGTCTTCAGGAGCAAACAC AAGCAAAGACAGACCACATGCCAGTGGTACTGATGGAGATGAAAGTGAGGAAGACCCACCTGAGCATAAG CCAAGCAAACTGAAGAGGAGCCATGAACTGGACATTGATGAAAACCCAGCTTCAGACTTTGATGACAGTG GTTCCCTTCTAGGATTCAAGTATGGCTCAGGACAAAAATATGGTGGAATCCCAAATTTCAGTCATCGGCA GGTCAGGTATTTAGAGAAGAATGTGAAGCTTAAGTCTAAGTACCTAGACATGCGCAGACAAATAAAGATG AAAAACAAACACATCTTCTTTACCAAAGAGTCAGAAAAACCATTTTTCAAGAAAAGCAAAATTCTGAGTA AGGTAGAAAAATTCCTCACATGGGTTAATAAACCAATGGATGAAGAAGCATCACAGGAATCATCTTCTCA TGACAATGTGCACGACGCTTCCACAAGTAGTGATTCAGAGGAACAAGACATGTCTGTTAAAAAAGGTGAT GACCTACTGGAGACTAATAATCCAGAACCTGAAAAGTGTCAGAGCGTATCTTCAGCTGGTGAACTTGAAA CAGAAAACTATGAAAGAGACAGCTTGCTAGCAACTGTTCCAGATGAGCAGGATTGTGTTACTCAAGAAGT GCCAGACTCCCGCCAGGCAGAAACTGAAGCTGAAGTGAAAAAGAAGAAGAACAAGAAGAAGAACAAAAAG GTGAATGGTCTGCCTCCTGAAATAGCTGCTGTTCCTGAGCTGGCAAAATACTGGGCCCAGAGGTACAGGC TCTTCTCCCGTTTTGATGATGGGATTAAGTTGGACAGAGAGGGCTGGTTTTCAGTTACACCCGAGAAGAT TGCTGAACACATTGCTGGCCGTGTTAGTCAGTCCTTCAAGTGTGACGTTGTAGTAGACGCATTCTGTGGA GTTGGAGGAAATACCATTCAGTTTGCCTTAACAGGAATGAGAGTGATTGCCATTGATATCGATCCTGTTA AGATTGCCCTTGCTCGCAATAATGCAGAAGTTTATGGGATAGCAGATAAGATAGAGTTCATCTGTGGAGA TTTCTTGCTGCTGGCTTCTTTTTTAAAGGCTGATGTTGTGTTCCTCAGCCCACCTTGGGGAGGGCCAGAC TATGCCACTGCAGAGACCTTTGACATTAGAACAATGATGTCTCCTGATGGCTTTGAAATTTTCAGACTTT CTAAGAAGATCACTAATAATATTGTTTATTTTCTTCCAAGAAATGCTGATATTGACCAGGTGGCATCCTT AGCTGGGCCTGGAGGGCAAGTGGAAATAGAACAGAACTTCCTTAACAACAAATTGAAGACAATCACTGCA TATTTTGGTGACCTAATTCGAAGACCAGCCTCTGAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221904 representing NM_024831
Red=Cloning site Green=Tags(s) MCCEKWSRVAEMFLFIEEREDCKILCLCSRAFVEDRKLYNLGLKGYYIRDSGNNSGDQATEEEEGGYSCG TAESHDSKGIGLDESELDSEAELMRSMGLPLQFGRITAHKDFEVSMNTRNKVKIKKKKHQKKYLDEIVQE SWRKEYEEDDILASDDPSSIEQYENTRTYELQSKKDTETENPPVENTLSPKLEITEKWEKYWNEYGGGLL WQSWQEKHPGQALSSEPWNFPDTKEEWEQHYSQLYWYYLEQFQYWEAQGWTFDASQSCDTDTYTSKTEAD DKNDEKCMKVDLVSFPSSPIMVDNDSSGTSDKDHSEILDGISNIKLNSEEVTQSQLDSCTSHDGHQQLSE VSSKRECPASGQSEPRNGGTNEESNSSGNTNTDPPAEDSQKSSGANTSKDRPHASGTDGDESEEDPPEHK PSKLKRSHELDIDENPASDFDDSGSLLGFKYGSGQKYGGIPNFSHRQVRYLEKNVKLKSKYLDMRRQIKM KNKHIFFTKESEKPFFKKSKILSKVEKFLTWVNKPMDEEASQESSSHDNVHDASTSSDSEEQDMSVKKGD DLLETNNPEPEKCQSVSSAGELETENYERDSLLATVPDEQDCVTQEVPDSRQAETEAEVKKKKNKKKNKK VNGLPPEIAAVPELAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCG VGGNTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPD YATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITA YFGDLIRRPASET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024831 |
| ORF Size | 2559 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024831.8 |
| RefSeq Size | 3708 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 96764 |
| UniProt ID | Q96RS0 |
| Protein Families | Druggable Genome |
| MW | 96.6 kDa |
| Gene Summary | Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221904L3 | Lenti-ORF clone of TGS1 (Myc-DDK-tagged)-Human trimethylguanosine synthase 1 (TGS1) |
CNY 10,830.00 |
|
| RC221904L4 | Lenti-ORF clone of TGS1 (mGFP-tagged)-Human trimethylguanosine synthase 1 (TGS1) |
CNY 10,830.00 |
|
| RG221904 | TGS1 (tGFP-tagged) - Human trimethylguanosine synthase 1 (TGS1) |
CNY 9,790.00 |
|
| SC108181 | TGS1 (untagged)-Human trimethylguanosine synthase 1 (TGS1) |
CNY 8,608.00 |
