DRP1 (DNM1L) (NM_012062) Human Tagged ORF Clone
CAT#: RC221708
DNM1L (Myc-DDK-tagged)-Human dynamin 1-like (DNM1L), transcript variant 1
ORF Plasmid: tGFP
"NM_012062" in other vectors (6)
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CNY 5,392.00
CNY 7,700.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; OPA5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221708 representing NM_012062
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCTAATTCCTGTCATAAACAAGCTCCAGGACGTCTTCAACACGGTGGGCGCCGACATCATCC AGCTGCCTCAAATCGTCGTAGTGGGAACGCAGAGCAGCGGAAAGAGCTCAGTGCTAGAAAGCCTGGTGGG GAGGGACCTGCTTCCCAGAGGTACTGGAATTGTCACCCGGAGACCTCTCATTCTGCAACTGGTCCATGTT TCACAAGAAGATAAACGGAAAACAACAGGAGAAGAAAATGGGGTGGAAGCAGAAGAATGGGGTAAATTTC TTCACACCAAAAATAAGCTTTACACGGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACAGAAAG AATTTCAGGAAATAATAAGGGAGTAAGCCCTGAACCAATTCATCTTAAGATTTTTTCACCCAACGTTGTC AATTTGACACTTGTGGATTTGCCAGGAATGACCAAGGTGCCTGTAGGTGATCAACCTAAGGATATTGAGC TTCAAATCAGAGAGCTCATTCTTCGGTTCATCAGTAATCCTAATTCCATTATCCTCGCTGTCACTGCTGC TAATACAGATATGGCAACATCAGAGGCACTTAAAATTTCAAGAGAGGTAGATCCAGATGGTCGCAGAACC CTAGCTGTAATCACTAAACTTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGG TTATTCCAGTCAAACTTGGAATAATTGGAGTAGTTAACAGGAGCCAGCTAGATATTAACAACAAGAAGAG TGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAATATCCATCTCTGGCCAATAGAAAT GGAACAAAGTATCTTGCTAGGACTCTAAACAGGTTACTGATGCATCACATCAGAGATTGTTTACCAGAGT TGAAAACAAGAATAAATGTTCTAGCTGCTCAGTATCAGTCTCTTCTAAATAGCTACGGTGAACCCGTGGA TGATAAAAGTGCTACTTTACTCCAACTTATTACCAAATTTGCCACAGAATATTGTAACACTATTGAAGGA ACTGCAAAATATATTGAAACTTCGGAGCTATGCGGTGGTGCTAGAATTTGTTATATTTTCCATGAGACTT TTGGGCGAACCTTAGAATCTGTTGATCCACTTGGTGGCCTTAACACTATTGACATTTTGACTGCCATTAG AAATGCTACTGGTCCTCGTCCTGCTTTATTTGTGCCTGAGGTTTCATTTGAGTTACTGGTGAAGCGGCAA ATCAAACGTCTAGAAGAGCCCAGCCTCCGCTGTGTGGAACTGGTTCATGAGGAAATGCAAAGGATCATTC AGCACTGTAGCAATTACAGTACACAGGAATTGTTACGATTTCCTAAACTTCATGATGCCATAGTTGAAGT GGTGACTTGTCTTCTTCGTAAAAGGTTGCCTGTTACAAATGAAATGGTCCATAACTTAGTGGCAATTGAA CTGGCTTATATCAACACAAAACATCCAGACTTTGCTGATGCTTGTGGGCTAATGAACAATAATATAGAGG AACAAAGGAGAAACAGGCTAGCCAGAGAATTACCTTCAGCTGTATCACGAGACAAGTCTTCTAAAGTTCC AAGTGCTTTGGCACCTGCCTCCCAGGAGCCCTCCCCCGCTGCTTCTGCTGAGGCTGATGGCAAGTTAATT CAGGACAGCAGAAGAGAAACTAAAAATGTTGCATCTGGAGGTGGTGGGGTTGGAGATGGTGTTCAAGAAC CAACCACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAGTTATTAGCAGAAGAAAAATC AAAACCCATTCCAATTATGCCAGCCAGTCCACAAAAAGGTCATGCCGTGAACCTGCTAGATGTGCCAGTT CCTGTTGCACGAAAACTATCTGCTCGGGAACAGCGAGATTGTGAGGTTATTGAACGACTCATTAAATCAT ATTTTCTCATTGTCAGAAAGAATATTCAAGACAGTGTGCCAAAGGCAGTAATGCATTTTTTGGTTAATCA TGTGAAAGACACTCTTCAGAGTGAGCTAGTAGGCCAGCTGTATAAATCATCCTTATTGGATGATCTTCTG ACAGAATCTGAGGACATGGCACAGCGCAGGAAAGAAGCAGCTGATATGCTAAAGGCATTACAAGGAGCCA GTCAAATTATTGCTGAAATCCGGGAGACTCATCTTTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221708 representing NM_012062
Red=Cloning site Green=Tags(s) MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHV SQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVV NLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRT LAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRN GTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEG TAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQ IKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIE LAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEPSPAASAEADGKLI QDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPV PVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLL TESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012062 |
ORF Size | 2208 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_012062.5 |
RefSeq Size | 3293 bp |
RefSeq ORF | 2211 bp |
Locus ID | 10059 |
UniProt ID | O00429 |
Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
MW | 81.7 kDa |
Gene Summary | This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221708L1 | Lenti ORF clone of Human dynamin 1-like (DNM1L), transcript variant 1, Myc-DDK-tagged |
CNY 7,792.00 |
|
RC221708L2 | Lenti ORF clone of Human dynamin 1-like (DNM1L), transcript variant 1, mGFP tagged |
CNY 7,792.00 |
|
RC221708L3 | Lenti ORF clone of Human dynamin 1-like (DNM1L), transcript variant 1, Myc-DDK-tagged |
CNY 9,600.00 |
|
RC221708L4 | Lenti ORF clone of Human dynamin 1-like (DNM1L), transcript variant 1, mGFP tagged |
CNY 9,600.00 |
|
RG221708 | DNM1L (tGFP-tagged) - Human dynamin 1-like (DNM1L), transcript variant 1 |
CNY 6,992.00 |
|
SC115574 | DNM1L (untagged)-Human dynamin 1-like (DNM1L), transcript variant 1 |
CNY 5,400.00 |