PRAX 1 (TSPOAP1) (NM_004758) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BZRAP1; PBR-IP; PRAX-1; PRAX1; RIM-BP1; RIMBP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC221525 representing NM_004758 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAACTGACAACCCTCCCACGGCCTGGGGACCCTGGAGCCATGGAGCCATGGGCACTGCCCACCT GGCATAGCTGGACTCCAGGTCGAGGGGGTGAACCTAGCAGTGCAGCCCCAAGCATCGCTGATACTCCTCC GGCAGCTCTGCAGCTTCAAGAACTGAGGTCTGAGGAGAGTTCCAAGCCCAAAGGAGACGGGAGCTCCAGG CCCGTGGGGGGAACTGACCCTGAAGGAGCAGAGGCTTGTCTGCCCAGCCTGGGCCAGCAAGCATCCAGCT CTGGACCCGCCTGCCAGAGGCCAGAGGATGAGGAAGTGGAGGCTTTCCTGAAGGCCAAGCTGAATATGAG CTTTGGGGACAGGCCCAATCTGGAGCTGCTGAGGGCCCTGGGGGAGCTGCGGCAGCGCTGTGCCATCCTT AAGGAGGAAAACCAGATGCTGAGGAAGAGCAGCTTCCCTGAGACAGAAGAGAAGGTGCGGAGGCTGAAGC GGAAGAACGCCGAGCTGGCGGTCATTGCCAAGCGCCTGGAGGAGAGGGCCCGAAAGCTGCAGGAAACGAA CCTGAGGGTGGTGAGTGCCCCCTTGCCCCGGCCGGGGACCAGCTTGGAGTTGTGTCGGAAGGCCCTAGCC CGCCAGCGAGCCCGGGACCTCAGTGAGACAGCCAGTGCACTGCTGGCCAAGGACAAGCAGATTGCTGCCT TGCAGCGGGAGTGCAGGGAGCTGCAGGCCAGGCTCACTCTGGTGGGCAAGGAGGGTCCCCAGTGGCTCCA CGTGCGGGACTTCGATCGGCTGCTGCGCGAGTCCCAGCGGGAGGTGCTGCGGCTGCAGAGGCAGATCGCG CTGCGCAACCAGCGGGAGACGCTCCCGCTCCCGCCGTCCTGGCCCCCGGGCCCTGCTCTCCAGGCCAGAG CAGGGGCGCCTGCTCCCGGGGCCCCGGGAGAGGCCACGCCCCAGGAGGATGCGGACAACCTACCCGTGAT TCTAGGGGAGCCAGAGAAAGAGCAGAGGGTGCAGCAGCTGGAATCGGAGCTCAGCAAGAAGCGGAAGAAA TGCGAGAGCCTGGAGCAGGAAGCCCGGAAAAAGCAGAGGCGATGTGAGGAGCTGGAACTGCAGCTGAGAC AAGCGCAGAATGAGAATGCCCGCCTGGTGGAGGAGAACTCCCGGCTCAGTGGGAGAGCCACAGAGAAGGA GCAGGTGGAGTGGGAGAATGCGGAGCTGAGGGGCCAGCTCCTGGGGGTGACACAGGAGAGGGACTCAGCC CTTCGCAAGAGCCAGGGCCTGCAGAGCAAGCTGGAGAGCCTGGAGCAAGTGCTGAAGCACATGCGGGAGG TGGCCCAGCGGCGGCAGCAGCTGGAGGTGGAGCATGAACAGGCTCGGCTCAGCCTACGGGAGAAGCAGGA GGAGGTCCGGAGACTGCAGCAGGCCCAGGCTGAAGCCCAGAGGGAACATGAAGGAGCCGTGCAGCTGCTG GAGTCTACCTTGGATTCCATGCAGGCCCGGGTTCGAGAGCTCGAAGAACAGTGCCGCAGCCAAACCGAGC AGTTCAGCCTCCTGGCACAAGAACTCCAGGCTTTCCGCCTGCACCCGGGCCCCTTGGATCTGCTCACATC TGCCCTGGACTGTGGGAGCCTTGGAGACTGCCCACCACCCCCCTGCTGCTGCTCCATTCCCCAGCCTTGC CGGGGGTCTGGCCCCAAAGACCTTGACCTCCCGCCGGGCTCCCCTGGGCGCTGCACCCCAAAGTCTTCCG AGCCTGCCCCTGCCACTCTCACTGGGGTCCCTCGAAGGACAGCCAAGAAGGCAGAGTCTCTCTCCAACTC CTCCCACTCCGAGTCCATCCACAACAGCCCCAAGTCATGCCCTACACCTGAGGTGGACACAGCCAGTGAG GTAGAGGAGCTGGAGGCAGACAGTGTCTCCCTGCTCCCAGCTGCGCCAGAGGGCAGCCGGGGAGGAGCCA GGATCCAGGTCTTCCTAGCACGTTATAGCTACAACCCCTTTGAGGGTCCCAATGAGAATCCAGAAGCAGA GCTTCCGCTGACAGCTGGCGAGTACATCTACATCTATGGCAACATGGATGAGGATGGCTTTTTTGAAGGA GAGCTCATGGATGGCCGAAGGGGCCTGGTCCCTTCCAATTTTGTAGAGCGTGTGTCGGATGATGACCTCC TGACCTCCCTCCCTCCAGAGCTGGCCGATTTGTCCCACAGCTCAGGCCCTGAACTCAGTTTCCTGAGTGT AGGTGGGGGTGGCAGCAGTAGCGGGGGCCAAAGCAGTGTGGGAAGGAGCCAGCCCAGACCTGAGGAGGAG GATGCAGGGGACGAGCTCAGTCTGAGCCCATCACCGGAGGGCCTGGGCGAGCCTCCTGCCGTGCCTTACC CCCGCCGTCTGGTGGTCCTCAAGCAGCTGGCCCACAGCGTGGTGCTGGCCTGGGAGCCGCCTCCTGAGCA AGTGGAGCTACACGGCTTCCATATCTGTGTGAATGGGGAGCTGCGACAGGCCCTGGGGCCTGGGGCGCCA CCCAAGGCCGTGCTGGAGAACCTGGACCTGTGGGCCGGGCCCCTTCACATTTCTGTCCAGGCCCTGACTA GCCGGGGCAGCTCTGACCCACTGCGCTGTTGCTTGGCGGTGGGTGCCCGGGCCGGAGTGGTGCCCAGCCA GCTGCGGGTCCATCGGTTGACAGCCACATCTGCTGAGATCACCTGGGTGCCCGGCAATAGCAACTTGGCC CATGCCATCTACCTCAATGGGGAAGAATGCCCACCTGCCAGCCCCAGTACCTACTGGGCCACCTTCTGCC ACTTACGGCCTGGCACACCCTATCAGGCCCAAGTGGAGGCTCAGCTCCCACCCCAAGGGCCCTGGGAACC AGGCTGGGAGAGGCTGGAGCAGCGGGCTGCCACCCTGCAGTTCACCACACTCCCAGCAGGCCCACCTGAT GCCCCTCTGGATGTGCAGATCGAGCCTGGGCCCTCCCCTGGGATCTTGATCATCAGTTGGCTCCCAGTCA CCATCGATGCTGCTGGCACATCCAACGGTGTCCGGGTCACAGGCTATGCCATCTACGCTGATGGGCAGAA GATCATGGAGGTGGCCTCACCCACGGCAGGCAGTGTACTGGTGGAGTTGTCCCAGCTGCAGCTGCTGCAG GTGTGTCGTGAGGTGGTCGTGCGCACCATGTCGCCCCACGGGGAGTCGGCGGACTCCATCCCGGCTCCTA TCACTCCCGCCCTGGCTCCGGCCAGCCTGCCAGCCCGAGTCTCCTGCCCCTCACCGCACCCAAGCCCAGA GGCCAGAGCGCCCCTTGCTTCAGCCTCCCCAGGGCCTGGAGACCCCAGCTCTCCTCTCCAGCACCCTGCT CCCCTTGGAACTCAAGAGCCTCCAGGAGCACCCCCTGCAAGCCCTTCCAGAGAGATGGCAAAAGGGTCCC ACGAGGACCCTCCAGCACCTTGCTCCCAGGAGGAGGCTGGGGCAGCAGTGCTGGGCACCTCAGAGGAGAG GACAGCCAGCACATCTACCCTGGGTGAGAAGGACCCTGGCCCCGCAGCTCCCTCACTGGCCAAGCAGGAG GCCGAGTGGACTGCAGGAGAGGCCTGTCCGGCCTCCAGCTCCACCCAGGGAGCACGGGCCCAGCAGGCGC CAAATACCGAGATGTGCCAAGGAGGAGACCCAGGGTCTGGGCTGAGGCCCAGGGCTGAGAAGGAGGACAC AGCAGAGCTTGGGGTTCATCTGGTGAACTCCCTCGTGGACCACGGCCGCAACTCAGACCTGTCAGACATC CAGGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGCTGGGTTCCAGGACTTGCTCCTTCCAGA AGCAGGTTGCTGGCAACAGCATCAGGGAGAATGGGGCCAAGTCCCAGCCCGACCCCTTTTGTGAGACTGA CAGCGATGAGGAGATCTTGGAGCAGATCCTGGAGCTGCCCCTCCAGCAGTTCTGTAGCAAGAAGCTCTTT AGCATCCCGGAGGAGGAGGAAGAGGAAGAGGAGGACGAGGAGGAGGAGAAGTCTGGGGCAGGCTGTTCTT CCCGAGACCCTGGCCCGCCTGAACCTGCATTGCTGGGGCTGGGCTGTGACAGTGGTCAGCCCCGAAGACC TGGCCAGTGTCCCTTGTCTCCTGAGTCCTCCAGGGCTGGAGACTGCCTGGAAGACATGCCTGGATTAGTT GGTGGAAGCAGCCGGAGGAGAGGAGGGGGCTCCCCTGAGAAGCCCCCAAGCCGCAGGCGGCCTCCAGATC CCCGAGAACACTGCAGCCGACTTCTCAGCAACAATGGGCCCCAGGCCTCTGGACGACTGGGCCCCACACG GGAGAGGGGTGGCCTCCCCGTAATTGAGGGCCCCAGGACTGGACTAGAGGCTAGCGGGAGAGGCCGGCTG GGCCCTTCCCGGAGGTGCTCCCGTGGCCGGGCGCTGGAGCCTGGCCTGGCCAGCTGCCTTTCCCCCAAGT GCTTGGAAATCAGCATTGAATATGATTCGGAGGATGAGCAGGAGGCGGGCAGCGGGGGCATCAGCATCAC CAGCTCCTGCTACCCTGGAGATAGGGAGGCCTGGGGCACAGCAACTGTAGGAAGGCCCAGGGGGCCTCCG AAGGCCAATTCAGGCCCCAAGCCCTACCCACGCCTCCCAGCCTGGGAGAAAGGGGAACCAGAGCGGAGAG GCCGCAGTGCGACGGGCAGAGCCAAGGAGCCACTCTCCCGGGCAACAGAGACCGGAGAGGCCAGAGGGCA GGACGGCTCTGGGCGGAGGGGCCCCCAGAAGAGAGGTGTCCGAGTCCTCAGGCCAAGCACTGCAGAGCTA GTCCCTGCGAGGAGCCCCTCAGAAACACTGGCTTACCAGCACCTACCCGTCAGGATCTTTGTGGCTCTGT TTGACTATGACCCCGTGTCAATGTCGCCCAATCCTGATGCTGGAGAAGAAGAGCTTCCCTTCCGAGAGGG TCAGATCCTGAAGGTGTTTGGGGACAAGGATGCCGATGGCTTCTACCAGGGCGAAGGTGGGGGCCGGACA GGCTACATTCCCTGCAACATGGTGGCTGAGGTGGCTGTGGACAGCCCTGCTGGGAGACAGCAACTGCTCC AGCGGGGTTATTTGTCCCCAGATATTCTCCTTGAGGGCTCAGGGAATGGTCCGTTTGTGTACTCCACAGC CCACACAACTGGGCCTCCTCCCAAGCCCCGCCGCTCCAAGAAAGCTGAGTCGGAAGGCCCTGCCCAGCCC TGTCCAGGCCCCCCTAAGCTGGTCCCCTCTGCTGACCTGAAAGCTCCCCACTCCATGGTGGCTGCATTTG ACTACAACCCCCAGGAGAGTTCCCCCAATATGGACGTGGAGGCAGAGCTGCCCTTCCGGGCAGGGGATGT CATTACTGTGTTTGGGGGCATGGACGATGACGGTTTCTACTATGGGGAATTAAATGGACAAAGGGGCCTG GTTCCATCCAACTTCCTGGAGGGCCCTGGGCCTGAGGCAGGCGGCCTGGACAGGGAACCCAGGACACCCC AGGCTGAGAGTCAGAGAACGAGGAGGAGAAGAGTCCAGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC221525 representing NM_004758 Red=Cloning site Green=Tags(s) MEQLTTLPRPGDPGAMEPWALPTWHSWTPGRGGEPSSAAPSIADTPPAALQLQELRSEESSKPKGDGSSR PVGGTDPEGAEACLPSLGQQASSSGPACQRPEDEEVEAFLKAKLNMSFGDRPNLELLRALGELRQRCAIL KEENQMLRKSSFPETEEKVRRLKRKNAELAVIAKRLEERARKLQETNLRVVSAPLPRPGTSLELCRKALA RQRARDLSETASALLAKDKQIAALQRECRELQARLTLVGKEGPQWLHVRDFDRLLRESQREVLRLQRQIA LRNQRETLPLPPSWPPGPALQARAGAPAPGAPGEATPQEDADNLPVILGEPEKEQRVQQLESELSKKRKK CESLEQEARKKQRRCEELELQLRQAQNENARLVEENSRLSGRATEKEQVEWENAELRGQLLGVTQERDSA LRKSQGLQSKLESLEQVLKHMREVAQRRQQLEVEHEQARLSLREKQEEVRRLQQAQAEAQREHEGAVQLL ESTLDSMQARVRELEEQCRSQTEQFSLLAQELQAFRLHPGPLDLLTSALDCGSLGDCPPPPCCCSIPQPC RGSGPKDLDLPPGSPGRCTPKSSEPAPATLTGVPRRTAKKAESLSNSSHSESIHNSPKSCPTPEVDTASE VEELEADSVSLLPAAPEGSRGGARIQVFLARYSYNPFEGPNENPEAELPLTAGEYIYIYGNMDEDGFFEG ELMDGRRGLVPSNFVERVSDDDLLTSLPPELADLSHSSGPELSFLSVGGGGSSSGGQSSVGRSQPRPEEE DAGDELSLSPSPEGLGEPPAVPYPRRLVVLKQLAHSVVLAWEPPPEQVELHGFHICVNGELRQALGPGAP PKAVLENLDLWAGPLHISVQALTSRGSSDPLRCCLAVGARAGVVPSQLRVHRLTATSAEITWVPGNSNLA HAIYLNGEECPPASPSTYWATFCHLRPGTPYQAQVEAQLPPQGPWEPGWERLEQRAATLQFTTLPAGPPD APLDVQIEPGPSPGILIISWLPVTIDAAGTSNGVRVTGYAIYADGQKIMEVASPTAGSVLVELSQLQLLQ VCREVVVRTMSPHGESADSIPAPITPALAPASLPARVSCPSPHPSPEARAPLASASPGPGDPSSPLQHPA PLGTQEPPGAPPASPSREMAKGSHEDPPAPCSQEEAGAAVLGTSEERTASTSTLGEKDPGPAAPSLAKQE AEWTAGEACPASSSTQGARAQQAPNTEMCQGGDPGSGLRPRAEKEDTAELGVHLVNSLVDHGRNSDLSDI QEEEEEEEEEEEEELGSRTCSFQKQVAGNSIRENGAKSQPDPFCETDSDEEILEQILELPLQQFCSKKLF SIPEEEEEEEEDEEEEKSGAGCSSRDPGPPEPALLGLGCDSGQPRRPGQCPLSPESSRAGDCLEDMPGLV GGSSRRRGGGSPEKPPSRRRPPDPREHCSRLLSNNGPQASGRLGPTRERGGLPVIEGPRTGLEASGRGRL GPSRRCSRGRALEPGLASCLSPKCLEISIEYDSEDEQEAGSGGISITSSCYPGDREAWGTATVGRPRGPP KANSGPKPYPRLPAWEKGEPERRGRSATGRAKEPLSRATETGEARGQDGSGRRGPQKRGVRVLRPSTAEL VPARSPSETLAYQHLPVRIFVALFDYDPVSMSPNPDAGEEELPFREGQILKVFGDKDADGFYQGEGGGRT GYIPCNMVAEVAVDSPAGRQQLLQRGYLSPDILLEGSGNGPFVYSTAHTTGPPPKPRRSKKAESEGPAQP CPGPPKLVPSADLKAPHSMVAAFDYNPQESSPNMDVEAELPFRAGDVITVFGGMDDDGFYYGELNGQRGL VPSNFLEGPGPEAGGLDREPRTPQAESQRTRRRRVQC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_004758
ORF Size	5571 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_004758.1, NP_004749.1
RefSeq Size	7033 bp
RefSeq ORF	5574 bp
Locus ID	9256
UniProt ID	O95153
Protein Families	Druggable Genome
MW	199.9 kDa

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