KIF3A (NM_007054) Human Tagged ORF Clone
CAT#: RC221331
- TrueORF®
KIF3A (Myc-DDK-tagged)-Human kinesin family member 3A (KIF3A)
ORF Plasmid: tGFP
"NM_007054" in other vectors (4)
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CNY 7,680.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FLA10; KLP-20 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221331 representing NM_007054
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATCAATAAATCAGAGAAGCCAGAAAGCTGCGATAATGTGAAGGTTGTTGTTAGGTGCCGGCCCC TCAATGAGAGAGAGAAATCAATGTGCTACAAACAGGCTGTCAGTGTGGATGAGATGAGGGGAACTATCAC TGTACATAAGACTGATTCTTCCAATGAACCTCCAAAGACATTTACTTTTGATACTGTTTTTGGACCAGAG AGTAAACAACTTGATGTTTATAACTTAACTGCAAGACCTATTATTGATTCTGTACTTGAAGGCTACAATG GGACTATTTTTGCATATGGACAAACCGGAACAGGCAAAACTTTTACCATGGAAGGTGTTCGAGCTATTCC TGAACTTAGAGGAATAATTCCCAATTCATTTGCTCACATATTTGGTCATATTGCAAAAGCGGAGGGTGAT ACAAGATTTTTGGTTCGAGTGTCTTATTTGGAAATATATAATGAAGAAGTTCGTGACCTTTTGGGCAAGG ATCAGACACAAAGGTTAGAGGTTAAAGAAAGACCTGATGTGGGAGTTTATATCAAAGATTTATCAGCTTA TGTGGTAAATAATGCTGATGATATGGATAGAATTATGACGCTAGGCCACAAAAATCGTTCTGTTGGTGCA ACTAATATGAACGAACATAGTTCCCGTTCCCATGCCATCTTTACAATTACTATAGAATGCAGTGAAAAAG GCATTGATGGTAACATGCATGTCAGGATGGGGAAGCTCCATCTTGTAGATCTTGCTGGTTCAGAAAGACA GGCAAAAACTGGAGCTACTGGACAGCGCCTAAAGGAAGCTACAAAAATCAATCTTTCACTTTCCACCCTT GGTAATGTAATTTCTGCCTTGGTTGATGGAAAAAGCACTCATGTGCCTTATCGTAACTCTAAACTGACTC GTCTTCTTCAGGATTCCTTAGGAGGAAATTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGATTA CAATTATGATGAAACTATCAGTACATTACGGTATGCCAATCGTGCTAAGAATATTAAAAATAAAGCTAGA ATTAATGAAGATCCAAAGGATGCTTTGCTGCGTCAGTTCCAGAAAGAAATAGAAGAACTGAAAAAGAAGC TTGAAGAAGGAGAAGAAATATCAGGCTCTGATATCAGTGGGTCAGAGGAAGATGATGATGAAGAGGGTGA GGTTGGAGAAGATGGAGAGAAAAGGAAAAAAAGAAGGGGAAAAAAGAAAGTCTCCCCAGACAAGATGATT GAAATGCAAGCAAAAATTGATGAGGAGAGAAAAGCACTTGAAACAAAGCTCGACATGGAAGAAGAAGAAA GAAACAAGGCTAGAGCTGAATTAGAGAAACGGGAAAAAGATCTTCTTAAAGCCCAACAAGAGCATCAGTC TTTGCTGGAAAAATTATCTGCCCTGGAAAAGAAGGTAATTGTTGGTGGGGTTGACTTGTTGGCCAAAGCT GAGGAACAAGAGAAACTTCTTGAAGAATCTAACATGGAACTGGAAGAAAGGAGGAAAAGAGCAGAGCAAC TTCGCAGAGAACTTGAGGAAAAAGAGCAAGAACGCTTGGATATTGAAGAAAAATATACCAGTTTGCAAGA GGAAGCACAGGGAAAGACCAAGAAGTTAAAGAAAGTTTGGACTATGCTGATGGCTGCAAAGTCAGAGATG GCTGATCTCCAACAAGAACATCAGAGGGAAATTGAAGGCCTACTGGAGAACATTCGGCAACTTAGCCGGG AGCTTCGACTTCAGATGCTTATTATTGATAACTTTATACCTCGGGATTATCAGGAAATGATTGAAAACTA TGTCCATTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTTGCTTATACAGGAAATAACATGAGG AAGCAAACCCCAGTACCTGATAAAAAGGAGAAAGATCCCTTTGAGGTGGACCTTTCTCACGTGTATCTTG CCTATACTGAGGAGAGTCTGCGTCAGTCTTTGATGAAACTAGAAAGACCACGAACTTCAAAGGGGAAAGC AAGGCCAAAGACAGGGAGAAGAAAGCGTTCTGCAAAGCCTGAAACTGTAATTGACTCTTTACTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221331 representing NM_007054
Red=Cloning site Green=Tags(s) MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPE SKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGD TRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGA TNMNEHSSRSHAIFTITIECSEKGIDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTL GNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKAR INEDPKDALLRQFQKEIEELKKKLEEGEEISGSDISGSEEDDDEEGEVGEDGEKRKKRRGKKKVSPDKMI EMQAKIDEERKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKA EEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEM ADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMR KQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007054 |
ORF Size | 2097 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007054.7 |
RefSeq Size | 6257 bp |
RefSeq ORF | 2100 bp |
Locus ID | 11127 |
UniProt ID | Q9Y496 |
Domains | kinesin |
Protein Families | Druggable Genome |
MW | 80 kDa |
Gene Summary | Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Functional Alterations in Ciliogenesis-Associated Kinase 1 (CILK1) that Result from Mutations Linked to Juvenile Myoclonic Epilepsy
,null,
Cells
,PubMed ID 32178256
[KIF3A]
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Documents
Product Manuals |
FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221331L3 | Lenti-ORF clone of KIF3A (Myc-DDK-tagged)-Human kinesin family member 3A (KIF3A) |
CNY 9,310.00 |
|
RC221331L4 | Lenti-ORF clone of KIF3A (mGFP-tagged)-Human kinesin family member 3A (KIF3A) |
CNY 9,310.00 |
|
RG221331 | KIF3A (tGFP-tagged) - Human kinesin family member 3A (KIF3A) |
CNY 9,280.00 |
|
SC115725 | KIF3A (untagged)-Human kinesin family member 3A (KIF3A) |
CNY 13,490.00 |