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A4GNT (NM_016161) Human Tagged ORF Clone
CAT＃: RC221256
A4GNT (Myc-DDK-tagged)-Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT)
ORF Plasmid: tGFP
"NM_016161" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | alpha4GnT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221256 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAAGGAGCTCCAGCTCTCCCTGTCAGTCACCTTGCTGCTTGTCTGTGGCTTCCTCTACCAGTTCA CCCTGAAGTCCAGCTGCCTCTTCTGCTTGCCTTCTTTCAAGTCCCACCAGGGGCTGGAAGCCCTCCTGAG CCACAGACGTGGCATTGTGTTTCTAGAGACCTCAGAGAGAATGGAGCCACCCCATTTGGTCTCCTGTTCC GTAGAGTCTGCTGCCAAGATTTATCCTGAGTGGCCTGTGGTGTTCTTTATGAAGGGTCTTACTGATTCCA CACCGATGCCCTCAAACTCCACATACCCAGCTTTTTCCTTCCTGTCAGCAATAGACAACGTTTTCCTCTT CCCTTTGGATATGAAAAGGCTGCTTGAAGACACACCATTGTTTTCATGGTACAATCAAATCAACGCCAGC GCAGAGAGAAACTGGCTCCACATCAGCTCGGATGCATCCCGCCTGGCCATCATCTGGAAATACGGTGGCA TCTACATGGACACCGATGTCATCTCCATCAGGCCCATCCCTGAGGAGAACTTTTTGGCTGCGCAGGCTTC TCGGTACTCTAGTAATGGAATATTTGGGTTCCTCCCCCACCACCCCTTTTTGTGGGAATGCATGGAAAAC TTTGTTGAACACTATAATTCAGACATTTGGGGCAACCAAGGCCCTGAGTTGATGACAAGGATGTTGAGGG TATGGTGTAAACTTGAAGACTTCCAGGAGGTGAGCGACCTCAGGTGTCTGAACATATCCTTCTTACACCC CCAAAGATTTTACCCCATCTCCTATCGAGAGTGGAGGCGCTACTATGAAGTGTGGGATACAGAGCCAAGC TTCAATGTCTCTTATGCCCTGCATTTGTGGAACCACATGAACCAGGAGGGGCGGGCTGTGATTAGAGGAA GCAACACACTGGTGGAAAATCTCTATCGCAAGCACTGTCCCAGGACTTACAGGGACCTGATTAAAGGCCC AGAGGGGTCAGTGACTGGGGAGCTGGGTCCAGGTAACAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221256 protein sequence
Red=Cloning site Green=Tags(s) MRKELQLSLSVTLLLVCGFLYQFTLKSSCLFCLPSFKSHQGLEALLSHRRGIVFLETSERMEPPHLVSCS VESAAKIYPEWPVVFFMKGLTDSTPMPSNSTYPAFSFLSAIDNVFLFPLDMKRLLEDTPLFSWYNQINAS AERNWLHISSDASRLAIIWKYGGIYMDTDVISIRPIPEENFLAAQASRYSSNGIFGFLPHHPFLWECMEN FVEHYNSDIWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPS FNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELGPGNK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016161 |
| ORF Size | 1020 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016161.3 |
| RefSeq Size | 1771 bp |
| RefSeq ORF | 1023 bp |
| Locus ID | 51146 |
| UniProt ID | Q9UNA3 |
| Protein Families | Transmembrane |
| MW | 39.5 kDa |
| Gene Summary | This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221256L3 | Lenti ORF clone of Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221256L4 | Lenti ORF clone of Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT), mGFP tagged |
CNY 5,890.00 |
|
| RG221256 | A4GNT (tGFP-tagged) - Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT) |
CNY 5,256.00 |
|
| SC304387 | A4GNT (untagged)-Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT) |
CNY 7,220.00 |
