Jagged 2 (JAG2) (NM_145159) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HJ2; SER2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC221039 representing NM_145159 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGCAGGGCCGGGGGCGCCTTCCCCGGCGGCTGCTGCTGCTGCTGGCGCTCTGGGTGCAGGCGG CGCGGCCCATGGGCTATTTCGAGCTGCAGCTGAGCGCGCTGCGGAACGTGAACGGGGAGCTGCTGAGCGG CGCCTGCTGTGACGGCGACGGCCGGACAACGCGCGCGGGGGGCTGCGGCCACGACGAGTGCGACACGTAC GTGCGCGTGTGCCTTAAGGAGTACCAGGCCAAGGTGACGCCCACGGGGCCCTGCAGCTACGGCCACGGCG CCACGCCCGTGCTGGGCGGCAACTCCTTCTACCTGCCGCCGGCGGGCGCTGCGGGGGACCGAGCGCGGGC GCGGGCCCGGGCCGGCGGCGACCAGGACCCGGGCCTCGTCGTCATCCCCTTCCAGTTCGCCTGGCCGCGC TCCTTTACCCTCATCGTGGAGGCCTGGGACTGGGACAACGATACCACCCCGAATGAGGAGCTGCTGATCG AGCGAGTGTCGCATGCCGGCATGATCAACCCGGAGGACCGCTGGAAGAGCCTGCACTTCAGCGGCCACGT GGCGCACCTGGAGCTGCAGATCCGCGTGCGCTGCGACGAGAACTACTACAGCGCCACTTGCAACAAGTTC TGCCGGCCCCGCAACGACTTTTTCGGCCACTACACCTGCGACCAGTACGGCAACAAGGCCTGCATGGACG GCTGGATGGGCAAGGAGTGCAAGGAAGCTGTGTGTAAACAAGGGTGTAATTTGCTCCACGGGGGATGCAC CGTGCCTGGGGAGTGCAGGTGCAGCTACGGCTGGCAAGGGAGGTTCTGCGATGAGTGTGTCCCCTACCCC GGCTGCGTGCATGGCAGTTGTGTGGAGCCCTGGCAGTGCAACTGTGAGACCAACTGGGGCGGCCTGCTCT GTGACAAAGACCTGAACTACTGTGGCAGCCACCACCCCTGCACCAACGGAGGCACGTGCATCAACGCCGA GCCTGACCAGTACCGCTGCACCTGCCCTGACGGCTACTCGGGCAGGAACTGTGAGAAGGCTGAGCACGCC TGCACCTCCAACCCGTGTGCCAACGGGGGCTCTTGCCATGAGGTGCCGTCCGGCTTCGAATGCCACTGCC CATCGGGCTGGAGCGGGCCCACCTGTGCCCTTGACATCGATGAGTGTGCTTCGAACCCGTGTGCGGCCGG TGGCACCTGTGTGGACCAGGTGGACGGCTTTGAGTGCATCTGCCCCGAGCAGTGGGTGGGGGCCACCTGC CAGCTGGACGTCAACGACTGTCGCGGGCAGTGTCAGCATGGGGGCACCTGCAAGGACCTGGTGAACGGGT ACCAGTGTGTGTGCCCACGGGGCTTCGGAGGCCGGCATTGCGAGCTGGAACGAGACGAGTGTGCCAGCAG CCCCTGCCACAGCGGCGGCCTCTGCGAGGACCTGGCCGACGGCTTCCACTGCCACTGCCCCCAGGGCTTC TCCGGGCCTCTCTGTGAGGTGGATGTCGACCTTTGTGAGCCAAGCCCCTGCCGGAACGGCGCTCGCTGCT ATAACCTGGAGGGTGACTATTACTGCGCCTGCCCTGATGACTTTGGTGGCAAGAACTGCTCCGTGCCCCG CGAGCCGTGCCCTGGCGGGGCCTGCAGAGTGATCGATGGCTGCGGGTCAGACGCGGGGCCTGGGATGCCT GGCACAGCAGCCTCCGGCGTGTGTGGCCCCCATGGACGCTGCGTCAGCCAGCCAGGGGGCAACTTTTCCT GCATCTGTGACAGTGGCTTTACTGGCACCTACTGCCATGAGAACATTGACGACTGCCTGGGCCAGCCCTG CCGCAATGGGGGCACATGCATCGATGAGGTGGACGCCTTCCGCTGCTTCTGCCCCAGCGGCTGGGAGGGC GAGCTCTGCGACACCAATCCCAACGACTGCCTTCCCGATCCCTGCCACAGCCGCGGCCGCTGCTACGACC TGGTCAATGACTTCTACTGTGCGTGCGACGACGGCTGGAAGGGCAAGACCTGCCACTCACGCGAGTTCCA GTGCGATGCCTACACCTGCAGCAACGGTGGCACCTGCTACGACAGCGGCGACACCTTCCGCTGCGCCTGC CCCCCCGGCTGGAAGGGCAGCACCTGCGCCGTCGCCAAGAACAGCAGCTGCCTGCCCAACCCCTGTGTGA ATGGTGGCACCTGCGTGGGCAGCGGGGCCTCCTTCTCCTGCATCTGCCGGGACGGCTGGGAGGGTCGTAC TTGCACTCACAATACCAACGACTGCAACCCTCTGCCTTGCTACAATGGTGGCATCTGTGTTGACGGCGTC AACTGGTTCCGCTGCGAGTGTGCACCTGGCTTCGCGGGGCCTGACTGCCGCATCAACATCGACGAGTGCC AGTCCTCGCCCTGTGCCTACGGGGCCACGTGTGTGGATGAGATCAACGGGTATCGCTGTAGCTGCCCACC CGGCCGAGCCGGCCCCCGGTGCCAGGAAGTGATCGGGTTCGGGAGATCCTGCTGGTCCCGGGGCACTCCG TTCCCACACGGAAGCTCCTGGGTGGAAGACTGCAACAGCTGCCGCTGCCTGGATGGCCGCCGTGACTGCA GCAAGGTGTGGTGCGGATGGAAGCCTTGTCTGCTGGCCGGCCAGCCCGAGGCCCTGAGCGCCCAGTGCCC ACTGGGGCAAAGGTGCCTGGAGAAGGCCCCAGGCCAGTGTCTGCGACCACCCTGTGAGGCCTGGGGGGAG TGCGGCGCAGAAGAGCCACCGAGCACCCCCTGCCTGCCACGCTCCGGCCACCTGGACAATAACTGTGCCC GCCTCACCTTGCATTTCAACCGTGACCACGTGCCCCAGGGCACCACGGTGGGCGCCATTTGCTCCGGGAT CCGCTCCCTGCCAGCCACAAGGGCTGTGGCACGGGACCGCCTGCTGGTGTTGCTTTGCGACCGGGCGTCC TCGGGGGCCAGTGCTGTGGAGGTGGCCGTGTCCTTCAGCCCTGCCAGGGACCTGCCTGACAGCAGCCTGA TCCAGGGCGCGGCCCACGCCATCGTGGCCGCCATCACCCAGCGGGGGAACAGCTCACTGCTCCTGGCTGT CACCGAGGTCAAGGTGGAGACGGTTGTTACGGGCGGCTCTTCCACAGGTCTGCTGGTGCCTGTGCTGTGT GGTGCCTTCAGCGTGCTGTGGCTGGCGTGCGTGGTCCTGTGCGTGTGGTGGACACGCAAGCGCAGGAAAG AGCGGGAGAGGAGCCGGCTGCCGCGGGAGGAGAGCGCCAACAACCAGTGGGCCCCGCTCAACCCCATCCG CAACCCCATTGAGCGGCCGGGGGGCCACAAGGACGTGCTCTACCAGTGCAAGAACTTCACGCCGCCGCCG CGCAGGGCGGACGAGGCGCTGCCCGGGCCGGCCGGCCACGCGGCCGTCAGGGAGGATGAGGAGGACGAGG ATCTGGGCCGCGGTGAGGAGGACTCCCTGGAGGCGGAGAAGTTCCTCTCACACAAATTCACCAAAGATCC TGGCCGCTCGCCGGGGAGGCCGGCCCACTGGGCCTCAGGCCCCAAAGTGGACAACCGCGCGGTCAGGAGC ATCAATGAGGCCCGCTACGCCGGCAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC221039 representing NM_145159 Red=Cloning site Green=Tags(s) MRAQGRGRLPRRLLLLLALWVQAARPMGYFELQLSALRNVNGELLSGACCDGDGRTTRAGGCGHDECDTY VRVCLKEYQAKVTPTGPCSYGHGATPVLGGNSFYLPPAGAAGDRARARARAGGDQDPGLVVIPFQFAWPR SFTLIVEAWDWDNDTTPNEELLIERVSHAGMINPEDRWKSLHFSGHVAHLELQIRVRCDENYYSATCNKF CRPRNDFFGHYTCDQYGNKACMDGWMGKECKEAVCKQGCNLLHGGCTVPGECRCSYGWQGRFCDECVPYP GCVHGSCVEPWQCNCETNWGGLLCDKDLNYCGSHHPCTNGGTCINAEPDQYRCTCPDGYSGRNCEKAEHA CTSNPCANGGSCHEVPSGFECHCPSGWSGPTCALDIDECASNPCAAGGTCVDQVDGFECICPEQWVGATC QLDVNDCRGQCQHGGTCKDLVNGYQCVCPRGFGGRHCELERDECASSPCHSGGLCEDLADGFHCHCPQGF SGPLCEVDVDLCEPSPCRNGARCYNLEGDYYCACPDDFGGKNCSVPREPCPGGACRVIDGCGSDAGPGMP GTAASGVCGPHGRCVSQPGGNFSCICDSGFTGTYCHENIDDCLGQPCRNGGTCIDEVDAFRCFCPSGWEG ELCDTNPNDCLPDPCHSRGRCYDLVNDFYCACDDGWKGKTCHSREFQCDAYTCSNGGTCYDSGDTFRCAC PPGWKGSTCAVAKNSSCLPNPCVNGGTCVGSGASFSCICRDGWEGRTCTHNTNDCNPLPCYNGGICVDGV NWFRCECAPGFAGPDCRINIDECQSSPCAYGATCVDEINGYRCSCPPGRAGPRCQEVIGFGRSCWSRGTP FPHGSSWVEDCNSCRCLDGRRDCSKVWCGWKPCLLAGQPEALSAQCPLGQRCLEKAPGQCLRPPCEAWGE CGAEEPPSTPCLPRSGHLDNNCARLTLHFNRDHVPQGTTVGAICSGIRSLPATRAVARDRLLVLLCDRAS SGASAVEVAVSFSPARDLPDSSLIQGAAHAIVAAITQRGNSSLLLAVTEVKVETVVTGGSSTGLLVPVLC GAFSVLWLACVVLCVWWTRKRRKERERSRLPREESANNQWAPLNPIRNPIERPGGHKDVLYQCKNFTPPP RRADEALPGPAGHAAVREDEEDEDLGRGEEDSLEAEKFLSHKFTKDPGRSPGRPAHWASGPKVDNRAVRS INEARYAGKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_145159
ORF Size	3600 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_145159.3
RefSeq Size	5721 bp
RefSeq ORF	3603 bp
Locus ID	3714
UniProt ID	Q9Y219
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Notch signaling pathway
MW	129.2 kDa
Gene Summary	The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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