GPCR 2037 (GPR151) (NM_194251) Human Tagged ORF Clone

CAT#: RC220856

  • TrueORF®

GPR151 (Myc-DDK-tagged)-Human G protein-coupled receptor 151 (GPR151)

ORF Plasmid: DDK tGFP



  "NM_194251" in other vectors (6)

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CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


GPR151 mouse monoclonal antibody,clone OTI3D1
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GALR4; GALRL; GPCR; GPCR-2037; PGR7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220856 representing NM_194251
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCAGCTGCCTTTGCAGACTCTAACTCCAGCAGCATGAATGTGTCCTTTGCTCACCTCCACTTTG
CCGGAGGGTACCTGCCCTCTGATTCCCAGGACTGGAGAACCATCATCCCGGCTCTCTTGGTGGCTGTCTG
CCTGGTGGGCTTCGTGGGAAACCTGTGTGTGATTGGCATCCTCCTTCACAATGCTTGGAAAGGAAAGCCA
TCCATGATCCACTCCCTGATTCTGAATCTCAGCCTGGCTGATCTCTCCCTCCTGCTGTTTTCTGCACCTA
TCCGAGCTACGGCGTACTCCAAAAGTGTTTGGGATCTAGGCTGGTTTGTCTGCAAGTCCTCTGACTGGTT
TATCCACACATGCATGGCAGCCAAGAGCCTGACAATCGTTGTGGTGGCCAAAGTATGCTTCATGTATGCA
AGTGACCCAGCCAAGCAAGTGAGTATCCACAACTACACCATCTGGTCAGTGCTGGTGGCCATCTGGACTG
TGGCTAGCCTGTTACCCCTGCCGGAATGGTTCTTTAGCACCATCAGGCATCATGAAGGTGTGGAAATGTG
CCTCGTGGATGTACCAGCTGTGGCTGAAGAGTTTATGTCGATGTTTGGTAAGCTCTACCCACTCCTGGCA
TTTGGCCTTCCATTATTTTTTGCCAGCTTTTATTTCTGGAGAGCTTATGACCAATGTAAAAAACGAGGAA
CTAAGACTCAAAATCTTAGAAACCAGATACGCTCAAAGCAAGTCACAGTGATGCTGCTGAGCATTGCCAT
CATCTCTGCTCTCTTGTGGCTCCCCGAATGGGTAGCTTGGCTGTGGGTATGGCATCTGAAGGCTGCAGGC
CCGGCCCCACCACAAGGTTTCATAGCCCTGTCTCAAGTCTTGATGTTTTCCATCTCTTCAGCAAATCCTC
TCATTTTTCTTGTGATGTCGGAAGAGTTCAGGGAAGGCTTGAAAGGTGTATGGAAATGGATGATAACCAA
AAAACCTCCAACTGTCTCAGAGTCTCAGGAAACACCAGCTGGCAACTCAGAGGGTCTTCCTGACAAGGTT
CCATCTCCAGAATCCCCAGCATCCATACCAGAAAAAGAGAAACCCAGCTCTCCCTCCTCTGGCAAAGGGA
AAACTGAGAAGGCAGAGATTCCCATCCTTCCTGACGTAGAGCAGTTTTGGCATGAGAGGGACACAGTCCC
TTCTGTACAGGACAATGACCCTATCCCCTGGGAACATGAAGATCAAGAGACAGGGGAAGGTGTTAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220856 representing NM_194251
Red=Cloning site Green=Tags(s)

MLAAAFADSNSSSMNVSFAHLHFAGGYLPSDSQDWRTIIPALLVAVCLVGFVGNLCVIGILLHNAWKGKP
SMIHSLILNLSLADLSLLLFSAPIRATAYSKSVWDLGWFVCKSSDWFIHTCMAAKSLTIVVVAKVCFMYA
SDPAKQVSIHNYTIWSVLVAIWTVASLLPLPEWFFSTIRHHEGVEMCLVDVPAVAEEFMSMFGKLYPLLA
FGLPLFFASFYFWRAYDQCKKRGTKTQNLRNQIRSKQVTVMLLSIAIISALLWLPEWVAWLWVWHLKAAG
PAPPQGFIALSQVLMFSISSANPLIFLVMSEEFREGLKGVWKWMITKKPPTVSESQETPAGNSEGLPDKV
PSPESPASIPEKEKPSSPSSGKGKTEKAEIPILPDVEQFWHERDTVPSVQDNDPIPWEHEDQETGEGVK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_194251
ORF Size 1257 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_194251.3
RefSeq Size 1260 bp
RefSeq ORF 1260 bp
Locus ID 134391
UniProt ID Q8TDV0
Protein Families Druggable Genome, Transmembrane
MW 46.5 kDa
Gene Summary This gene encodes an orphan member of the class A rhodopsin-like family of G-protein-coupled receptors (GPCRs). Within the rhodopsin-like family, this gene is a member of the SOG subfamily that includes somatostatin, opioid, galanin, and kisspeptin receptors. The orthologous mouse gene has a restricted pattern of neuronal expression which is induced following nerve injury. All GPCRs have a transmembrane domain that includes seven transmembrane alpha-helices. A general feature of GPCR signaling is the agonist-induced conformational change in the receptor, leading to activation of the heterotrimeric G protein. The activated G protein then binds to and activates numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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