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GATA6 (NM_005257) Human Tagged ORF Clone
CAT＃: RC220721
- TrueORF®
GATA6 (Myc-DDK-tagged)-Human GATA binding protein 6 (GATA6)
ORF Plasmid: tGFP
"NM_005257" in other vectors (6)
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CNY 6,648.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 4,070.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220721 representing NM_005257
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGACTGACGGCGGCTGGTGCTTGCCGAAGCGCTTCGGGGCCGCGGGTGCGGACGCCAGCGACT CCAGAGCCTTTCCAGCGCGGGAGCCCTCCACGCCGCCTTCCCCCATCTCTTCCTCGTCCTCCTCCTGCTC CCGGGGCGGAGAGCGGGGCCCCGGCGGCGCCAGCAACTGCGGGACGCCTCAGCTCGACACGGAGGCGGCG GCCGGACCCCCGGCCCGCTCGCTGCTGCTCAGTTCCTACGCTTCGCATCCCTTCGGGGCTCCCCACGGAC CTTCGGCGCCTGGGGTCGCGGGCCCCGGGGGCAACCTGTCGAGCTGGGAGGACTTGCTGCTGTTCACTGA CCTCGACCAAGCCGCGACCGCCAGCAAGCTGCTGTGGTCCAGCCGCGGCGCCAAGCTGAGCCCCTTCGCA CCCGAGCAGCCGGAGGAGATGTACCAGACCCTCGCCGCTCTCTCCAGCCAGGGTCCGGCCGCCTACGACG GCGCGCCCGGCGGCTTCGTGCACTCTGCGGCCGCGGCGGCAGCAGCCGCGGCGGCGGCCAGCTCCCCGGT CTACGTGCCCACCACCCGCGTGGGTTCCATGCTGCCCGGCCTACCGTACCACCTGCAGGGGTCGGGCAGT GGGCCAGCCAACCACGCGGGCGGCGCGGGCGCGCACCCCGGCTGGCCTCAGGCCTCGGCCGACAGCCCTC CATACGGCAGCGGAGGCGGCGCGGCTGGCGGCGGGGCCGCGGGGCCTGGCGGCGCTGGCTCAGCCGCGGC GCACGTCTCGGCGCGCTTCCCCTACTCTCCCAGCCCGCCCATGGCCAACGGCGCCGCGCGGGAGCCGGGA GGCTACGCGGCGGCGGGCAGTGGGGGCGCGGGAGGCGTGAGCGGCGGCGGCAGTAGCCTGGCGGCCATGG GCGGCCGCGAGCCCCAGTACAGCTCGCTGTCGGCCGCGCGGCCGCTGAACGGGACGTACCACCACCACCA CCACCACCACCACCACCATCCGAGCCCCTACTCGCCCTACGTGGGGGCGCCACTGACGCCTGCCTGGCCC GCCGGACCCTTCGAGACCCCGGTGCTGCACAGCCTGCAGAGCCGCGCCGGAGCCCCGCTCCCGGTGCCCC GGGGTCCCAGTGCAGACCTGCTGGAGGACCTGTCCGAGAGCCGCGAGTGCGTGAACTGCGGCTCCATCCA GACGCCGCTGTGGCGGCGGGACGGCACCGGCCACTACCTGTGCAACGCCTGCGGGCTCTACAGCAAGATG AACGGCCTCAGCCGGCCCCTCATCAAGCCGCAGAAGCGCGTGCCTTCATCACGGCGGCTTGGATTGTCCT GTGCCAACTGTCACACCACAACTACCACCTTATGGCGCAGAAACGCCGAGGGTGAACCCGTGTGCAATGC TTGTGGACTCTACATGAAACTCCATGGGGTGCCCAGACCACTTGCTATGAAAAAAGAGGGAATTCAAACC AGGAAACGAAAACCTAAGAACATAAATAAATCAAAGACTTGCTCTGGTAATAGCAATAATTCCATTCCCA TGACTCCAACTTCCACCTCTTCTAACTCAGATGATTGCAGCAAAAATACTTCCCCCACAACACAACCTAC AGCCTCAGGGGCGGGTGCCCCGGTGATGACTGGTGCGGGAGAGAGCACCAATCCCGAGAACAGCGAGCTC AAGTATTCGGGTCAAGATGGGCTCTACATAGGCGTCAGTCTCGCCTCGCCGGCCGAAGTCACGTCCTCCG TGCGACCGGATTCCTGGTGCGCCCTGGCCCTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220721 representing NM_005257
Red=Cloning site Green=Tags(s) MALTDGGWCLPKRFGAAGADASDSRAFPAREPSTPPSPISSSSSSCSRGGERGPGGASNCGTPQLDTEAA AGPPARSLLLSSYASHPFGAPHGPSAPGVAGPGGNLSSWEDLLLFTDLDQAATASKLLWSSRGAKLSPFA PEQPEEMYQTLAALSSQGPAAYDGAPGGFVHSAAAAAAAAAAASSPVYVPTTRVGSMLPGLPYHLQGSGS GPANHAGGAGAHPGWPQASADSPPYGSGGGAAGGGAAGPGGAGSAAAHVSARFPYSPSPPMANGAAREPG GYAAAGSGGAGGVSGGGSSLAAMGGREPQYSSLSAARPLNGTYHHHHHHHHHHPSPYSPYVGAPLTPAWP AGPFETPVLHSLQSRAGAPLPVPRGPSADLLEDLSESRECVNCGSIQTPLWRRDGTGHYLCNACGLYSKM NGLSRPLIKPQKRVPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQT RKRKPKNINKSKTCSGNSNNSIPMTPTSTSSNSDDCSKNTSPTTQPTASGAGAPVMTGAGESTNPENSEL KYSGQDGLYIGVSLASPAEVTSSVRPDSWCALALA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005257 |
| ORF Size | 1785 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005257.6 |
| RefSeq Size | 3494 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 2627 |
| UniProt ID | Q92908 |
| Domains | GATA, GATA-N |
| Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors |
| MW | 59.9 kDa |
| Gene Summary | This gene is a member of a small family of zinc finger transcription factors that play an important role in the regulation of cellular differentiation and organogenesis during vertebrate development. This gene is expressed during early embryogenesis and localizes to endo- and mesodermally derived cells during later embryogenesis and thereby plays an important role in gut, lung, and heart development. Mutations in this gene are associated with several congenital defects. [provided by RefSeq, Mar 2012] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MicroRNA-203 diminishes the stemness of human colon cancer cells by suppressing GATA6 expression
,Lai, HT;Tseng, WK;Huang, SW;Chao, TC;Su, Y;,
J. Cell. Physiol.
,PubMed ID 31544978
[GATA6]
|
|
Novel GATA6 Mutations in Patients with Pancreatic Agenesis and Congenital Heart Malformations
,Chao, CS;McKnight, KD;Cox, KL;Chang, AL;Kim, SK;,
PloS one
[GATA6]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220721L1 | Lenti-ORF clone of GATA6 (Myc-DDK-tagged)-Human GATA binding protein 6 (GATA6) |
CNY 9,048.00 |
|
| RC220721L2 | Lenti-ORF clone of GATA6 (mGFP-tagged)-Human GATA binding protein 6 (GATA6) |
CNY 7,130.00 |
|
| RC220721L3 | Lenti-ORF clone of GATA6 (Myc-DDK-tagged)-Human GATA binding protein 6 (GATA6) |
CNY 9,048.00 |
|
| RC220721L4 | Lenti-ORF clone of GATA6 (mGFP-tagged)-Human GATA binding protein 6 (GATA6) |
CNY 9,048.00 |
|
| RG220721 | GATA6 (tGFP-tagged) - Human GATA binding protein 6 (GATA6) |
CNY 8,248.00 |
|
| SC116877 | GATA6 (untagged)-Human GATA binding protein 6 (GATA6) |
CNY 6,656.00 |
