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SPRED1 (NM_152594) Human Tagged ORF Clone
CAT＃: RC220714
SPRED1 (Myc-DDK-tagged)-Human sprouty-related, EVH1 domain containing 1 (SPRED1)
ORF Plasmid: tGFP
"NM_152594" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hSpred1; LGSS; NFLS; PPP1R147; spred-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220714 representing NM_152594
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGGAGACGGCGACTTCTGACAACGATAATAGTTATGCACGAGTGCGAGCTGTGGTGATGACCC GAGATGACTCAAGTGGTGGATGGTTACCACTTGGAGGGAGTGGACTAAGCAGCGTCACTGTCTTCAAAGT CCCTCATCAGGAAGAGAATGGCTGTGCTGACTTTTTTATCCGTGGAGAGCGACTCAGGGACAAAATGGTG GTTTTGGAATGTATGCTTAAAAAAGACCTCATTTATAATAAGGTCACTCCAACATTTCACCACTGGAAGA TTGATGACAAAAAGTTTGGTCTTACGTTTCAAAGTCCTGCTGATGCTAGGGCTTTTGATAGAGGTATCCG AAGAGCTATAGAGGATATTTCTCAAGGATGCCCCGAATCAAAAAATGAAGCTGAAGGGGCAGATGACTTA CAAGCAAATGAAGAGGATTCTTCCAGTTCTCTAGTGAAGGATCACCTTTTTCAGCAAGAGACAGTTGTTA CCAGTGAGCCTTATAGAAGCTCAAATATAAGACCTTCTCCCTTTGAAGATCTGAATGCCAGAAGAGTCTA CATGCAAAGCCAAGCCAATCAGATAACATTTGGTCAGCCAGGCTTGGACATTCAGAGCAGAAGTATGGAA TACGTACAGCGGCAAATATCCAAGGAATGTGGAAGCCTAAAGTCCCAAAATAGGGTCCCTTTGAAATCAA TCAGACATGTCAGCTTTCAAGATGAGGATGAGATTGTCAGAATAAACCCTCGAGATATCTTAATACGTCG CTATGCAGACTACAGACATCCTGACATGTGGAAAAATGACTTGGAAAGAGATGATGCTGATTCCAGTATT CAGTTTTCTAAACCAGACAGTAAAAAATCAGACTATCTGTACTCTTGTGGGGATGAGACTAAGTTAAGTT CACCCAAAGACTCTGTGGTATTTAAGACGCAGCCTTCCTCATTAAAAATTAAGAAGTCAAAACGAAGAAA AGAGGATGGTGAACGTTCTCGCTGCGTATACTGCCAGGAAAGGTTTAATCATGAAGAAAATGTCAGGGGA AAATGTCAGGATGCTCCAGACCCTATTAAAAGATGCATATATCAAGTTAGTTGCATGCTCTGTGCAGAGA GCATGTTGTATCATTGTATGTCAGACTCAGAGGGAGATTTTTCTGATCCCTGTTCGTGTGACACTAGCGA CGACAAGTTCTGCTTGCGATGGTTAGCCCTGGTAGCTTTGTCTTTCATTGTACCATGTATGTGCTGCTAC GTCCCTTTGAGAATGTGCCATCGCTGTGGTGAGGCATGTGGTTGCTGTGGTGGGAAACATAAAGCTGCTG GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220714 representing NM_152594
Red=Cloning site Green=Tags(s) MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENGCADFFIRGERLRDKMV VLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDL QANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSME YVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSI QFSKPDSKKSDYLYSCGDETKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRG KCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCY VPLRMCHRCGEACGCCGGKHKAAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152594 |
| ORF Size | 1332 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152594.1, NP_689807.1 |
| RefSeq Size | 3816 bp |
| RefSeq ORF | 1335 bp |
| Locus ID | 161742 |
| UniProt ID | Q7Z699 |
| Domains | WH1 |
| Protein Pathways | Jak-STAT signaling pathway |
| MW | 50.3 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Striatal microRNA controls cocaine intake through CREB signalling
,Jonathan A. Hollander, Heh-In Im, Antonio L. Amelio, Jannet Kocerha, Purva Bali, Qun Lu, David Willoughby, Claes Wahlestedt, Michael D. Conkright, Paul J. Kenny,
Nature 466, 197-202 (8 July 2010) doi:10.1038/nature09202 Article
[SPRED1]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220714L1 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC220714L2 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), mGFP tagged |
CNY 5,890.00 |
|
| RC220714L3 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220714L4 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 1 (SPRED1), mGFP tagged |
CNY 5,890.00 |
|
| RG220714 | SPRED1 (tGFP-tagged) - Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 5,256.00 |
|
| SC101247 | SPRED1 (untagged)-Human sprouty-related, EVH1 domain containing 1 (SPRED1) |
CNY 3,656.00 |
