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PLA2G4E (NM_001080490) Human Tagged ORF Clone
CAT＃: RC220690
PLA2G4E (Myc-DDK-tagged)-Human phospholipase A2, group IVE (PLA2G4E)
ORF Plasmid: tGFP
"NM_001080490" in other vectors (4)
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CNY 6,144.00
CNY 8,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FLJ45651; MGC126633; MGC126661 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220690 representing NM_001080490
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGCCAAGTGCATTACAGAACCAGTAGGTATAGAGCTGGCACCCTGCCTGCAGGCTCCCAGCGTAC CGGAGACAGACCTGAAGGGTGTGGTCCAGGCCCGGGGTGGGGGGGCCAGTGTTCTGGAAAAGCCAAGGGA AGGGTTCAAGAGGGCTGAGCAGGTTCCTGTGAGCCAGACAGACTGTTTTGTGAGCCTCTGGCTGCCCACC GCCTCTCAGAAGAAGCTGAGGACAAGGACCATCTCCAACTGCCCAAATCCAGAGTGGAATGAAAGCTTCA ACTTCCAGATCCAGAGCCGAGTGAAGAACGTGCTAGAGTTGAGTGTCTGTGATGAAGACACAGTGACACC AGATGACCATCTCCTGACAGTTCTCTATGACCTCACCAAGCTCTGTTTCCGAAAGAAAACCCACGTGAAG TTTCCACTCAACCCGCAGGGCATGGAAGAGCTGGAGGTGGAGTTCCTGCTGGAGGAGAGTCCCTCTCCAC CTGAGACCCTCGTCACCAATGGCGTGCTGGTGTCTCGACAAGTCTCCTGCCTGGAGGTTCATGCACAATC CAGGAGGCGGAGGAAGAGGGAGAAAATGAAGGACCTCCTGGTGATGGTGAACGAATCCTTTGAGAACACC CAGCGTGTCCGGCCCTGCTTGGAACCCTGCTGCCCAACCTCTGCCTGCTTCCAAACCGCTGCCTGCTTCC ACTACCCCAAGTACTTCCAGTCCCAGGTGCACGTGGAAGTGCCCAAGAGTCACTGGAGCTGTGGGCTTTG CTGCCGCTCTCGCAAGAAGGGCCCCATCAGCCAGCCCCTCGACTGCCTTTCCGATGGTCAGGTGATGACC CTGCCTGTGGGTGAGAGTTATGAATTACACATGAAGTCTACACCCTGCCCTGAGACACTGGACGTGCGGC TGGGCTTCAGCCTGTGCCCAGCAGAGCTGGAGTTTCTGCAGAAGCGGAAGGTCGTGGTGGCCAAGGCCCT GAAGCAGGTGCTGCAGCTGGAGGAAGACCTGCAGGAGGACGAGGTGCCGCTGATAGCCATCATGGCCACT GGGGGTGGAACAAGATCCATGACCTCCATGTATGGCCACCTGCTGGGGCTGCAGAAGCTGAACCTCCTGG ACTGTGCCAGCTACATCACCGGTCTATCAGGGGCCACCTGGACCATGGCTACCTTGTACCGTGACCCTGA CTGGTCCTCCAAAAACTTGGAGCCTGCTATCTTTGAGGCTCGGAGACATGTGGTAAAGGACAAGCTACCC TCCCTGTTCCCAGACCAGCTCCGCAAATTCCAGGAGGAGCTCCGGCAGCGCAGCCAGGAAGGCTACAGGG TCACCTTTACAGACTTCTGGGGCCTGCTGATAGAGACCTGCCTGGGGGACGAGAGAAATGAATGCAAACT GTCAGATCAGCGTGCTGCTTTGAGCTGCGGCCAGAACCCCCTGCCCATCTACCTCACCATCAATGTCAAG GATGATGTAAGCAACCAGGACTTCAGAGAGTGGTTCGAGTTCTCCCCCTACGAGGTGGGCCTGCAGAAGT ATGGGGCCTTCATCCCCTCCGAGCTCTTCGGCTCCGAGTTCTTCATGGGGCGGCTGGTGAAGAGGATCCC GGAGTCTCGAATCTGCTACATGCTAGGCCTGTGGAGCAGCATCTTCTCCCTGAACCTGCTGGATGCCTGG AACCTGTCACACACCTCGGAGGAGTTTTTCCACAGGTGGACAAGGGAGAAAGTGCAGGACATCGAAGACG AGCCGATCCTGCCTGAAATCCCCAAATGTGATGCTAACATCCTGGAGACCACGGTAGTGATCCCAGGGTC ATGGCTGTCCAATTCTTTCCGAGAAATCCTTACCCATCGGTCCTTCGTGTCTGAGTTTCACAACTTCCTG TCTGGGCTGCAGCTGCACACCAACTACCTCCAGAATGGCCAGTTCTCTAGGTGGAAAGACACAGTGCTAG ATGGTTTCCCAAACCAGCTGACCGAGTCCGCGAACCACCTGTGCCTGCTGGACACTGCGTTCTTTGTCAA CTCCAGCTACCCGCCCCTCCTCAGGCCAGAGCGAAAAGCCGACCTCATCATCCACCTCAACTACTGTGCT GGGTCCCAGACAAAGCCCCTGAAACAAACCTGTGAGTACTGCACTGTGCAGAACATCCCCTTCCCCAAAT ACGAGCTGCCAGATGAGAATGAAAATCTCAAGGAATGCTACCTGATGGAGAACCCCCAGGAACCCGATGC CCCCATCGTGACTTTCTTCCCACTCATCAATGACACTTTCCGAAAATACAAGGCACCAGGTGTAGAGCGA AGCCCTGAGGAGCTGGAGCAGGGCCAGGTGGACATTTATGGTCCCAAAACTCCCTATGCCACCAAGGAGC TGACATACACAGAGGCCACCTTTGACAAGCTGGTGAAACTCTCAGAGTATAACATCCTGAATAATAAGGA CACTCTCCTCCAGGCTCTGCGGCTCGCAGTGGAGAAGAAGAAGCGCCTGAAGGGCCAGTGTCCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220690 representing NM_001080490
Red=Cloning site Green=Tags(s) MRAKCITEPVGIELAPCLQAPSVPETDLKGVVQARGGGASVLEKPREGFKRAEQVPVSQTDCFVSLWLPT ASQKKLRTRTISNCPNPEWNESFNFQIQSRVKNVLELSVCDEDTVTPDDHLLTVLYDLTKLCFRKKTHVK FPLNPQGMEELEVEFLLEESPSPPETLVTNGVLVSRQVSCLEVHAQSRRRRKREKMKDLLVMVNESFENT QRVRPCLEPCCPTSACFQTAACFHYPKYFQSQVHVEVPKSHWSCGLCCRSRKKGPISQPLDCLSDGQVMT LPVGESYELHMKSTPCPETLDVRLGFSLCPAELEFLQKRKVVVAKALKQVLQLEEDLQEDEVPLIAIMAT GGGTRSMTSMYGHLLGLQKLNLLDCASYITGLSGATWTMATLYRDPDWSSKNLEPAIFEARRHVVKDKLP SLFPDQLRKFQEELRQRSQEGYRVTFTDFWGLLIETCLGDERNECKLSDQRAALSCGQNPLPIYLTINVK DDVSNQDFREWFEFSPYEVGLQKYGAFIPSELFGSEFFMGRLVKRIPESRICYMLGLWSSIFSLNLLDAW NLSHTSEEFFHRWTREKVQDIEDEPILPEIPKCDANILETTVVIPGSWLSNSFREILTHRSFVSEFHNFL SGLQLHTNYLQNGQFSRWKDTVLDGFPNQLTESANHLCLLDTAFFVNSSYPPLLRPERKADLIIHLNYCA GSQTKPLKQTCEYCTVQNIPFPKYELPDENENLKECYLMENPQEPDAPIVTFFPLINDTFRKYKAPGVER SPEELEQGQVDIYGPKTPYATKELTYTEATFDKLVKLSEYNILNNKDTLLQALRLAVEKKKRLKGQCPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080490 |
| ORF Size | 2517 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080490.1, NP_001073959.1 |
| RefSeq Size | 2520 bp |
| RefSeq ORF | 2519 bp |
| Locus ID | 123745 |
| Protein Pathways | alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway |
| MW | 95.7 kDa |
| Gene Summary | This gene encodes a member of the cytosolic phospholipase A2 group IV family. Members of this family are involved in regulation of membrane tubule-mediated transport. The enzyme encoded by this member of the family plays a role in trafficking through the clathrin-independent endocytic pathway. The enzyme regulates the recycling process via formation of tubules that transport internalized clathrin-independent cargo proteins back to the cell surface. [provided by RefSeq, Jan 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220690L3 | Lenti ORF clone of Human phospholipase A2, group IVE (PLA2G4E), Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC220690L4 | Lenti ORF clone of Human phospholipase A2, group IVE (PLA2G4E), mGFP tagged |
CNY 10,260.00 |
|
| RG220690 | PLA2G4E (tGFP-tagged) - Human phospholipase A2, group IVE (PLA2G4E) |
CNY 7,744.00 |
|
| SC315840 | PLA2G4E (untagged)-Human phospholipase A2, group IVE (PLA2G4E) |
CNY 16,250.00 |
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