FTCD (NM_206965) Human Tagged ORF Clone

CAT#: RC220581

FTCD (Myc-DDK-tagged)-Human formiminotransferase cyclodeaminase (FTCD), transcript variant A

ORF Plasmid: DDK tGFP



  "NM_206965" in other vectors (4)

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CNY 4,032.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


FTCD mouse monoclonal antibody, clone OTI4A7 (formerly 4A7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms LCHC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220581 representing NM_206965
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCAGCTGGTGGAATGCGTCCCCAACTTTTCGGAGGGGAAGAACCAGGAGGTGATCGACGCCATCT
CTGGAGCCATCACACAGACCCCGGGCTGCGTGCTGCTGGATGTGGACGCAGGCCCTTCCACCAACCGCAC
CGTGTACACCTTCGTGGGGCCGCCGGAGTGCGTGGTGGAGGGGGCCCTCAACGCTGCCCGGGTAGCTTCC
CGACTTATCGACATGAGCAGGCACCAAGGAGAGCACCCCCGCATGGGGGCCCTAGACGTCTGCCCCTTCA
TCCCCGTGAGGGGCGTCAGCGTGGATGAGTGTGTGCTCTGCGCCCAGGCCTTTGGCCAGAGGCTGGCAGA
GGAGCTGGACGTGCCAGTTTACCTGTACGGCGAGGCAGCCAGGATGGACAGTCGCCGGACCCTGCCGGCC
ATCCGGGCCGGGGAGTACGAGGCCCTCCCTAAGAAGCTCCAGCAGGCCGACTGGGCGCCCGACTTTGGTC
CCAGCTCCTTTGTCCCCAGTTGGGGGGCCACGGCCACGGGGGCGAGGAAGTTCCTCATTGCTTTTAACAT
CAACCTGCTCGGCACAAAGGAGCAAGCCCACCGCATCGCGCTCAACCTGCGGGAGCAGGGCCGCGGGAAG
GACCAGCCAGGACGTCTGAAGAAAGTTCAGGGCATTGGCTGGTACCTGGATGAGAAGAACCTGGCTCAGG
TGTCCACCAATCTTCTGGACTTTGAGGTCACGGCACTGCACACGGTCTACGAGGAGACCTGCCGAGAAGC
ACAGGAGCTGAGCCTCCCAGTGGTGGGCTCACAGCTGGTGGGCCTGGTGCCCCTGAAGGCTCTGCTGGAT
GCGGCCGCCTTCTACTGCGAGAAGGAGAACCTCTTCATCCTGGAGGAGGAGCAGCGGATCAGGCTGGTGG
TGAGCCGGCTGGGCCTGGACTCCCTGTGCCCCTTCAGCCCTAAGGAGCGGATCATCGAGTACCTGGTCCC
TGAGCGCGGGCCTGAGCGAGGCCTGGGCAGCAAGTCCCTGCGCGCCTTCGTGGGGGAGGTGGGTGCCCGC
TCTGCGGCCCCCGGGGGCGGCTCGGTGGCGGCGGCCGCTGCGGCCATGGGTGCGGCGCTGGGCTCCATGG
TGGGCCTCATGACCTACGGGCGGCGCCAATTCCAGTCCCTGGACACGACGATGCGGCGCCTGATCCCGCC
CTTCCGCGAGGCTTCGGCCAAGCTAACCACGCTGGTGGATGCCGACGCCGAGGCCTTCACCGCCTACCTG
GAAGCAATGAGGCTCCCCAAGAACACACCTGAGGAAAAGGACAGGCGCACGGCGGCCCTACAGGAGGGTC
TGAGGCGGGCAGTCTCTGTGCCGCTGACGCTGGCGGAGACGGTGGCCTCGCTGTGGCCGGCCCTGCAGGA
ACTGGCCCGGTGTGGGAACCTGGCCTGCCGGTCAGACCTCCAGGTGGCGGCCAAAGCCCTGGAGATGGGC
GTGTTTGGCGCATATTTCAACGTGCTCATCAACCTGAGGGACATCACAGACGAGGCATTTAAGGACCAGA
TCCACCATCGTGTTTCCAGCCTCCTGCAGGAAGCCAAGACCCAGGCTGCACTGGTGCTGGACTGCTTGGA
GACCCGGCAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220581 representing NM_206965
Red=Cloning site Green=Tags(s)

MSQLVECVPNFSEGKNQEVIDAISGAITQTPGCVLLDVDAGPSTNRTVYTFVGPPECVVEGALNAARVAS
RLIDMSRHQGEHPRMGALDVCPFIPVRGVSVDECVLCAQAFGQRLAEELDVPVYLYGEAARMDSRRTLPA
IRAGEYEALPKKLQQADWAPDFGPSSFVPSWGATATGARKFLIAFNINLLGTKEQAHRIALNLREQGRGK
DQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLD
AAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGAR
SAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYL
EAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMG
VFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_206965
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_206965.2
RefSeq Size 1884 bp
RefSeq ORF 1626 bp
Locus ID 10841
UniProt ID O95954
Protein Pathways Histidine metabolism, Metabolic pathways, One carbon pool by folate
MW 58.7 kDa
Gene Summary The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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