HIPK2 (NM_022740) Human Tagged ORF Clone
CAT#: RC220278
HIPK2 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1
ORF Plasmid: tGFP
"NM_022740" in other vectors (7)
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CNY 8,344.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PRO0593 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220278 representing NM_022740.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGT GCCTTCTGTAGTGTGAAGAAACTGAAAATAGAGCCGAGTTCCAACTGGGACATGACTGGGTACGGCTCC CACAGCAAAGTGTATAGCCAGAGCAAGAACATCCCCCTGTCGCAGCCAGCCACCACAACCGTCAGCACC TCCTTGCCGGTCCCAAACCCAAGCCTACCTTACGAGCAGACCATCGTCTTCCCAGGAAGCACCGGGCAC ATCGTGGTCACCTCAGCAAGCAGCACTTCTGTCACCGGGCAAGTCCTCGGCGGACCACACAACCTAATG CGTCGAAGCACTGTGAGCCTCCTTGATACCTACCAAAAATGTGGACTCAAGCGTAAGAGCGAGGAGATC GAGAACACAAGCAGCGTGCAGATCATCGAGGAGCATCCACCCATGATTCAGAATAATGCAAGCGGGGCC ACTGTCGCCACTGCCACCACGTCTACTGCCACCTCCAAAAACAGCGGCTCCAACAGCGAGGGCGACTAT CAGCTGGTGCAGCATGAGGTGCTGTGCTCCATGACCAACACCTACGAGGTCTTAGAGTTCTTGGGCCGA GGGACGTTTGGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAGATCGTAGCCATCAAGATCCTG AAGAACCACCCATCCTATGCCCGACAAGGTCAGATTGAAGTGAGCATCCTGGCCCGGTTGAGCACGGAG AGTGCCGATGACTATAACTTCGTCCGGGCCTACGAATGCTTCCAGCACAAGAACCACACGTGCTTGGTC TTCGAGATGTTGGAGCAGAACCTCTATGACTTTCTGAAGCAAAACAAGTTTAGCCCCTTGCCCCTCAAA TACATTCGCCCAGTTCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTAGGTCTTATCCAC GCTGACCTCAAACCAGAAAACATCATGCTGGTGGATCCATCTAGACAACCATACAGAGTCAAGGTCATC GACTTTGGTTCAGCCAGCCACGTCTCCAAGGCTGTGTGCTCCACCTACTTGCAGTCCAGATATTACAGG GCCCCTGAGATCATCCTTGGTTTACCATTTTGTGAGGCAATTGACATGTGGTCCCTGGGCTGTGTTATT GCAGAATTGTTCCTGGGTTGGCCGTTATATCCAGGAGCTTCGGAGTATGATCAGATTCGGTATATTTCA CAAACACAGGGTTTGCCTGCTGAATATTTATTAAGCGCCGGGACAAAGACAACTAGGTTTTTCAACCGT GACACGGACTCACCATATCCTTTGTGGAGACTGAAGACACCAGATGACCATGAAGCAGAGACAGGGATT AAGTCAAAAGAAGCAAGAAAGTACATTTTCAACTGTTTAGATGATATGGCCCAGGTGAACATGACGACA GATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTCATTGACCTGTTGAAGAAG ATGCTGACCATTGATGCTGACAAGAGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATG ACACACTTACTCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATCTGCAAG CGTCGGGTGAATATGTATGACACGGTGAACCAGAGCAAAACCCCTTTCATCACGCACGTGGCCCCCAGC ACGTCCACCAACCTGACCATGACCTTTAACAACCAGCTGACCACTGTCCACAACCAGGCTCCCTCCTCT ACCAGTGCCACTATTTCCTTAGCCAATCCCGAAGTCTCCATACTAAACTACCCATCTACACTCTACCAG CCCTCAGCGGCATCCATGGCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGATT TGTGCCCGGCCTGACCCGTTCCAGCAAGCTCTCATCGTGTGTCCCCCCGGCTTCCAAGGCTTGCAGGCC TCTCCCTCTAAGCACGCTGGCTACTCGGTGCGAATGGAAAATGCAGTTCCCATCGTCACTCAAGCCCCA GGAGCTCAGCCTCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGACCCAGCAG ATCCTGCTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCCACCCACACATCAGTGCAGCATGCCACC GTGATTCCCGAGACCATGGCAGGCACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGC CATTATAATCCCATCATGCAGCAGCCTGCACTATTGACCGGTCATGTGACCCTTCCAGCAGCACAGCCC TTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCAGCCAACCAGCACCACCTCCTCCCGGAAGAGTAAG CAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCCCCA CAGCGATCCAAGCGTGTCAAGGAGAACACACCTCCCCGCTGTGCCATGGTGCACAGTAGCCCGGCCTGC AGCACCTCGGTCACCTGTGGGTGGGGCGACGTGGCCTCCAGCACCACCCGGGAACGGCAGCGGCAGACA ATTGTCATTCCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCATCAGCAGTGACACGGACGAGGAG GAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGCAAAGAAAAAACGTCATCAGCTGTGTCACA GTCCACGACTCCCCCTACTCCGACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGG CACAACAATGCCAATGCCTTTGACACCAAGGGGAGCCTGGAGAATCACTGCACGGGGAACCCCCGAACC ATCATCGTGCCACCCCTGAAAACCCAGGCCAGCGAAGTATTGGTGGAGTGTGATAGCCTGGTGCCAGTC AACACCAGTCACCACTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCAGCGGTCAC TCTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGGCCGGGCCCCCACTTCCAGCAGCAG CAGCCACTCAATCTCAGCCAGGCTCAGCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAG CAGGCCTACATCACTCCCACCATGGCCCAGGCTCCGTACTCCTTCCCGCACAACAGCCCCAGCCACGGC ACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGCCCACCTCCCCACCCAGCCCCACCTCTACACC TACACTGCGCCGGCGGCCCTGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCG CGCCACACCGTGCAGCACACTGCCTACCCAGCCAGCATCGTCCACCAGGTCCCCGTGAGCATGGGCCCC CGGGTCCTGCCCTCGCCCACCATCCACCCGAGTCAGTATCCAGCCCAATTTGCCCACCAGACCTACATC AGCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGCCCCGCCAAGGTCAACCAGTACCCT TACATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC220278
Blue=ORF Red=Cloning site Green=Tag(s) MAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKIEPSSNWDMTGYGSHSKVYSQSKNIPLSQPATTTVST SLPVPNPSLPYEQTIVFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEI ENTSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGR GTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLV FEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVI DFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYIS QTQGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTT DLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICK RRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQAPSSTSATISLANPEVSILNYPSTLYQ PSAASMAAVAQRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAP GAQPLQIQPGLLAQQAWPSGTQQILLPPAWQQLTGVATHTSVQHATVIPETMAGTQQLADWRNTHAHGS HYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKSKQHQSSVRNVSTCEVSSSQAISSP QRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTTRERQRQTIVIPDTPSPTVSVITISSDTDEE EEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSVQQRAGHNNANAFDTKGSLENHCTGNPRT IIVPPLKTQASEVLVECDSLVPVNTSHHSSSYKSKSSSNVTSTSGHSSGSSSGAITYRQQRPGPHFQQQ QPLNLSQAQQHITTDRTGSHRRQQAYITPTMAQAPYSFPHNSPSHGTVHPHLAAAAAAAHLPTQPHLYT YTAPAALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYPAQFAHQTYI SASPASTVYTGYPLSPAKVNQYPYI TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC220278 also available, TP320278 |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022740 |
ORF Size | 3594 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022740.5 |
RefSeq Size | 15148 bp |
RefSeq ORF | 3597 bp |
Locus ID | 28996 |
UniProt ID | Q9H2X6 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase, Transcription Factors |
MW | 131 kDa |
Gene Summary | This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Exosomal miR-1260b derived from non-small cell lung cancer promotes tumor metastasis through the inhibition of HIPK2
,null,
Cell Death & Disease
,PubMed ID 34321461
[HIPK2]
|
A novel isoform of Homeodomain-interacting protein kinase-2 promotes YAP/TEAD transcriptional activity in NSCLC cells
,Dai, Y;Kyoyama, H;Yang, YL;Wang, Y;Liu, S;Wang, Y;Mao, JH;Xu, Z;Uematsu, K;Jablons, DM;You, L;,
Oncotarget
,PubMed ID 33613845
[HIPK2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220278L1 | Lenti ORF clone of Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1, Myc-DDK-tagged |
CNY 10,744.00 |
|
RC220278L2 | Lenti ORF clone of Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1, mGFP tagged |
CNY 13,870.00 |
|
RC220278L3 | Lenti ORF clone of Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1, Myc-DDK-tagged |
CNY 13,870.00 |
|
RC220278L4 | Lenti ORF clone of Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1, mGFP tagged |
CNY 10,744.00 |
|
RG220278 | HIPK2 (tGFP-tagged) - Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1 |
CNY 13,210.00 |
|
SC311119 | HIPK2 (untagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1 |
CNY 23,090.00 |
|
SC318547 | HIPK2 (untagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1 |
CNY 23,090.00 |