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BAG2 (NM_004282) Human Tagged ORF Clone
CAT＃: RC220122
BAG2 (Myc-DDK-tagged)-Human BCL2-associated athanogene 2 (BAG2)
ORF Plasmid: tGFP
"NM_004282" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BAG-2; dJ417I1.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGCGAAGATCAACGCTAAAGCCAACGAGGGGCGCTTCTGCCGCTCCTCCTCCATGGCTGACC GCTCCAGCCGCCTGCTGGAGAGCCTGGACCAGCTGGAGCTCAGGGTTGAAGCTTTGAGAGAAGCAGCAAC TGCTGTTGAGCAAGAGAAAGAAATCCTTCTGGAAATGATCCACAGTATCCAAAATAGCCAGGACATGAGG CAGATCAGTGACGGAGAAAGAGAAGAATTAAATCTGACTGCAAACCGTTTGATGGGAAGAACTCTCACCG TTGAAGTGTCAGTAGAAACAATTAGAAACCCCCAGCAGCAAGAATCCCTAAAGCATGCCACAAGGATTAT TGATGAGGTGGTCAATAAGTTTCTGGATGATTTGGGAAATGCCAAGAGTCATTTAATGTCGCTCTACAGT GCATGTTCATCTGAGGTGCCACATGGGCCAGTTGATCAGAAGTTTCAATCCATAGTAATTGGCTGTGCTC TTGAAGATCAGAAGAAAATTAAGAGAAGATTAGAGACTCTGCTTAGAAATATTGAAAACTCTGACAAGGC CATCAAGCTATTAGAGCATTCTAAAGGAGCTGGTTCCAAAACTCTGCAACAAAATGCTGAAAGCAGATTC AAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220122 protein sequence
Red=Cloning site Green=Tags(s) MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMR QISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSHLMSLYS ACSSEVPHGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSKTLQQNAESRF N TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004282 |
| ORF Size | 633 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004282.4 |
| RefSeq Size | 2068 bp |
| RefSeq ORF | 636 bp |
| Locus ID | 9532 |
| UniProt ID | O95816 |
| Protein Families | Druggable Genome |
| MW | 23.8 kDa |
| Gene Summary | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220122L1 | Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC220122L2 | Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), mGFP tagged |
CNY 5,890.00 |
|
| RC220122L3 | Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC220122L4 | Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), mGFP tagged |
CNY 5,890.00 |
|
| RG220122 | BAG2 (tGFP-tagged) - Human BCL2-associated athanogene 2 (BAG2) |
CNY 4,000.00 |
|
| SC303461 | BAG2 (untagged)-Human BCL2-associated athanogene 2 (BAG2) |
CNY 2,400.00 |
