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CLASP2 (NM_015097) Human Tagged ORF Clone
CAT＃: RC220105
- TrueORF®
CLASP2 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 2 (CLASP2), transcript variant 1
ORF Plasmid: tGFP
"NM_015097" in other vectors (4)
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CNY 15,456.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220105 representing NM_015097
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCGCAGCATGGAGTACTTCTGCGCCCAGGTGCAGCAGAAGGACGTCGGCGGCCGGCTGCAGG TCGGCCAGGAGCTCCTGCTCTACCTTGGCGCCCCCGGCGCCATCTCGGACCTGGAGGAGGACCTGGGCCG CCTAGGCAAGACAGTCGACGCGCTCACCGGCTGGGTGGGTTCGAGCAACTACCGGGTATCATTAATGGGA TTGGAAATTTTAAGTGCCTTTGTGGACAGATTATCAACACGCTTTAAATCCTATGTAGCAATGGTTATTG TAGCTTTAATAGACAGAATGGGAGATGCCAAAGACAAGGTTCGAGATGAAGCTCAGACTCTGATATTGAA GTTAATGGATCAAGTAGCACCACCTATGTACATTTGGGAGCAGTTGGCTTCTGGTTTTAAACACAAGAAT TTTCGATCTCGAGAAGGCGTGTGTCTGTGTCTTATTGAAACCTTAAACATTTTTGGGGCTCAGCCACTAG TCATCAGCAAATTGATACCACATTTGTGTATCCTGTTTGGAGACTCCAACAGTCAGGTGAGAGATGCTGC AATATTGGCTATAGTGGAGATTTATAGACATGTGGGAGAAAAAGTGAGGATGGATCTTTATAAGAGAGGA ATTCCCCCTGCTAGATTAGAAATGATATTTGCCAAATTTGATGAAGTGCAAAGTTCAGGCGGTATGATTT TGAGTGTCTGCAAAGATAAAAGCTTCGATGATGAAGAATCAGTGGATGGAAATAGGCCATCATCAGCTGC ATCAGCCTTCAAGGTTCCTGCACCTAAAACATCCGGAAATCCTGCCAACAGTGCAAGGAAGCCTGGTTCA GCAGGTGGCCCTAAGGTTGGAGGTGCTTCTAAGGAAGGAGGTGCTGGAGCAGTTGATGAAGATGATTTTA TAAAAGCTTTTACAGATGTCCCTTCTATTCAGATTTATTCTAGTCGAGAACTCGAAGAAACATTAAATAA AATCAGGGAAATTTTGTCAGATGATAAACATGACTGGGATCAGCGTGCCAATGCACTGAAGAAAATTCGA TCACTGCTTGTTGCTGGAGCTGCACAGTATGATTGCTTTTTTCAACATTTACGATTGTTGGATGGAGCAC TTAAACTTTCAGCTAAGGATCTTAGATCCCAGGTGGTTAGAGAAGCTTGTATTACTGTAGCCCACCTTTC AACAGTTTTGGGAAACAAGTTTGATCATGGCGCTGAAGCCATTGTACCTACACTTTTTAATCTCGTCCCC AATAGTGCAAAAGTCATGGCAACTTCTGGATGTGCAGCAATCAGATTTATCATTCGGCATACTCATGTAC CCAGACTTATACCTTTAATAACAAGCAATTGCACATCAAAATCAGTTCCCGTGAGGAGACGTTCATTTGA ATTTTTAGATTTATTGTTGCAAGAGTGGCAGACTCATTCATTGGAAAGACATGCAGCCGTCTTGGTTGAA ACTATTAAAAAGGGAATTCATGATGCTGACGCTGAGGCCAGAGTGGAGGCAAGAAAGACATACATGGGTC TTAGAAACCACTTTCCTGGTGAAGCTGAAACATTATATAATTCCCTTGAGCCATCTTATCAGAAGAGTCT TCAAACTTACTTAAAGAGTTCTGGCAGTGTAGCATCTCTTCCACAATCAGACAGGTCCTCATCCAGCTCA CAGGAAAGTCTCAATCGCCCTTTTTCTTCCAAATGGTCTACAGCAAATCCATCAACTGTGGCTGGAAGAG TATCAGCAGGCAGCAGCAAAGCCAGTTCCCTTCCAGGAAGCCTGCAGCGTTCACGAAGTGACATTGATGT GAATGCTGCTGCAGGTGCCAAGGCACATCATGCTGCTGGACAGTCTGTGCGAAGCGGGCGCTTAGGTGCA GGTGCCCTGAATGCAGGTTCCTATGCGTCACTAGAGGATACTTCTGACAAGCTGGATGGAACAGCATCTG AAGATGGCCGGGTGAGAGCAAAACTTTCAGCACCACTTGCTGGCATGGGAAATGCCAAGGCAGATTCTAG AGGAAGAAGTCGAACAAAAATGGTGTCTCAATCACAGCCTGGTAGCCGGTCTGGGTCTCCAGGAAGAGTT CTGACCACAACAGCCCTGTCCACTGTGAGCTCTGGTGTTCAAAGAGTCCTGGTCAATTCAGCCTCAGCAC AAAAAAGAAGCAAGATACCACGGAGCCAGGGCTGTAGCAGAGAGGCTAGTCCATCTAGGCTTTCAGTGGC CCGAAGCAGTCGTATTCCTCGACCAAGTGTGAGTCAAGGATGCAGCCGGGAAGCTAGTCGGGAGAGCAGC AGAGACACAAGTCCTGTTCGCTCTTTTCAGCCCCTCGGTCCAGGTTATGGGATCAGCCAATCAAGTCGAC TGTCGTCTTCTGTTAGTGCCATGCGAGTCCTGAACACAGGTTCTGATGTGGAGGAGGCGGTGGCAGATGC CTTGAAAAAACCAGCTCGAAGAAGATATGAATCATATGGAATGCATTCAGATGATGACGCCAACAGCGAT GCATCTAGTGCTTGTTCAGAACGCTCCTATAGTTCTCGAAATGGTAGTATTCCTACATATATGAGGCAGA CGGAAGATGTGGCAGAAGTCCTCAATAGATGTGCTAGTTCCAATTGGTCAGAAAGGAAAGAAGGCCTCCT AGGTCTGCAGAACTTATTAAAAAATCAGAGAACACTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATT TTCACAAGAATGTTTGCTGACCCTCATGGCAAGAGAGTATTCAGCATGTTTTTGGAGACTCTAGTGGATT TCATACAAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTACTGCTGACACAACTACTAAAAAAAAT GGGTGCTGATTTGCTTGGATCTGTTCAGGCAAAAGTTCAGAAAGCCCTTGATGTTACAAGAGAGTCTTTT CCAAATGATCTTCAGTTCAATATTCTAATGAGATTTACAGTTGATCAGACCCAGACACCAAGCTTAAAGG TGAAGGTTGCTATCCTTAAATACATAGAAACTCTGGCCAAACAGATGGATCCAGGAGATTTTATAAATTC CAGTGAAACTCGCCTAGCAGTGTCTCGGGTCATCACTTGGACAACAGAACCCAAAAGTTCTGATGTTCGG AAGGCAGCACAGTCAGTGCTGATTTCATTATTTGAACTCAATACCCCAGAGTTTACAATGTTATTAGGAG CTTTACCAAAAACTTTTCAGGATGGTGCTACCAAGCTTCTTCATAATCACCTTCGAAACACTGGCAATGG AACCCAGAGTTCCATGGGGAGTCCTTTGACAAGACCAACACCACGATCACCAGCTAACTGGTCCAGTCCT CTTACTTCTCCTACCAATACATCACAGAATACTTTATCTCCAAGTGCATTTGATTATGACACAGAAAATA TGAACTCTGAAGATATTTATAGCTCTCTTAGAGGTGTCACTGAAGCAATCCAGAATTTCAGCTTCCGTAG CCAAGAAGATATGAATGAGCCATTGAAAAGGGATTCTAAAAAAGATGATGGCGATTCAATGTGTGGTGGT CCTGGGATGTCTGACCCAAGAGCAGGAGGTGATGCTACTGACTCAAGTCAAACAGCTCTTGATAATAAAG CTTCATTGCTCCATTCAATGCCTACTCACTCCTCTCCACGCTCTCGAGACTATAATCCATATAACTATTC AGATAGCATCAGTCCCTTCAACAAGTCTGCCCTCAAGGAAGCCATGTTTGATGATGATGCTGACCAGTTT CCTGACGATCTTTCCCTAGATCATTCTGACCTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAACCATAATG AGCGTGTAGAAGAAAGAAAAATTGCCCTCTATGAACTTATGAAACTGACACAGGAAGAATCTTTTAGTGT TTGGGATGAACACTTCAAAACAATATTGCTTTTATTGCTTGAAACGCTTGGAGATAAAGAGCCTACAATC AGGGCTTTGGCATTAAAGGTTTTAAGAGAAATCCTAAGGCATCAACCAGCAAGATTTAAAAACTATGCAG AATTGACTGTCATGAAAACATTGGAAGCACATAAAGATCCTCATAAGGAGGTGGTGAGATCTGCTGAGGA AGCGGCATCAGTGTTGGCCACTTCAATTAGTCCAGAGCAGTGCATCAAAGTGCTTTGTCCTATCATTCAA ACTGCAGACTACCCAATTAATCTGGCTGCAATCAAAATGCAAACAAAAGTGATAGAGAGAGTGTCCAAGG AAACCCTAAACCTGCTTTTGCCAGAGATTATGCCAGGTCTAATACAGGGTTATGATAATTCAGAGAGCAG TGTTCGGAAAGCTTGTGTCTTCTGCCTGGTGGCTGTTCATGCGGTAATTGGTGATGAACTAAAACCACAT CTCAGTCAACTTACTGGCAGTAAAATGAAGCTACTGAATCTTTACATCAAACGTGCACAAACAGGTTCTG GAGGAGCTGATCCCACTACTGATGTTTCTGGACAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220105 representing NM_015097
Red=Cloning site Green=Tags(s) MEPRSMEYFCAQVQQKDVGGRLQVGQELLLYLGAPGAISDLEEDLGRLGKTVDALTGWVGSSNYRVSLMG LEILSAFVDRLSTRFKSYVAMVIVALIDRMGDAKDKVRDEAQTLILKLMDQVAPPMYIWEQLASGFKHKN FRSREGVCLCLIETLNIFGAQPLVISKLIPHLCILFGDSNSQVRDAAILAIVEIYRHVGEKVRMDLYKRG IPPARLEMIFAKFDEVQSSGGMILSVCKDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGS AGGPKVGGASKEGGAGAVDEDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIR SLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVP NSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVE TIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSS QESLNRPFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVRSGRLGA GALNAGSYASLEDTSDKLDGTASEDGRVRAKLSAPLAGMGNAKADSRGRSRTKMVSQSQPGSRSGSPGRV LTTTALSTVSSGVQRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESS RDTSPVRSFQPLGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALKKPARRRYESYGMHSDDDANSD ASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEI FTRMFADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESF PNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVR KAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSP LTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMNEPLKRDSKKDDGDSMCGG PGMSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQF PDDLSLDHSDLVAELLKELSNHNERVEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTI RALALKVLREILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQ TADYPINLAAIKMQTKVIERVSKETLNLLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPH LSQLTGSKMKLLNLYIKRAQTGSGGADPTTDVSGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015097 |
| ORF Size | 4518 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015097.1, NP_055912.1 |
| RefSeq Size | 7289 bp |
| RefSeq ORF | 4548 bp |
| Locus ID | 23122 |
| UniProt ID | O75122 |
| MW | 164.7 kDa |
| Gene Summary | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220105L3 | Lenti ORF clone of Human cytoplasmic linker associated protein 2 (CLASP2), transcript variant 1, Myc-DDK-tagged |
CNY 16,910.00 |
|
| RC220105L4 | Lenti ORF clone of Human cytoplasmic linker associated protein 2 (CLASP2), transcript variant 1, mGFP tagged |
CNY 16,910.00 |
|
| RG220105 | CLASP2 (tGFP-tagged) - Human cytoplasmic linker associated protein 2 (CLASP2), transcript variant 1 |
CNY 16,530.00 |
|
| SC304229 | CLASP2 (untagged)-Human cytoplasmic linker associated protein 2 (CLASP2), transcript variant 1 |
CNY 15,464.00 |
