gamma Sarcoglycan (SGCG) (NM_000231) Human Tagged ORF Clone
CAT#: RC220093
SGCG (Myc-DDK-tagged)-Human sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (SGCG)
ORF Plasmid: tGFP
"NM_000231" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 35DAG; A4; DAGA4; DMDA; DMDA1; gamma-SG; LGMD2C; LGMDR5; MAM; SCARMD2; SCG3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220093 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCGTGAGCAGTACACTACAGCCACAGAAGGCATCTGCATAGAGAGGCCAGAGAATCAGTATGTCT ACAAAATTGGCATTTATGGCTGGAGAAAGCGCTGTCTCTACTTGTTTGTTCTTCTTTTACTCATCATCCT CGTTGTGAATTTAGCTCTTACAATTTGGATTCTTAAAGTGATGTGGTTTTCTCCAGCAGGAATGGGCCAC TTGTGTGTAACAAAAGATGGACTGCGCTTGGAAGGGGAATCAGAATTTTTATTCCCATTGTATGCCAAAG AAATACACTCCAGAGTGGACTCATCTCTGCTTCTACAATCAACCCAGAATGTGACTGTAAATGCGCGCAA CTCAGAAGGGGAGGTCACAGGCAGGTTAAAAGTCGGTCCCAAAATGGTAGAAGTCCAGAATCAACAGTTT CAGATCAACTCCAACGACGGCAAGCCACTATTTACTGTAGATGAGAAGGAAGTTGTGGTTGGTACAGATA AACTTCGAGTAACTGGGCCTGAAGGGGCTCTTTTTGAACATTCAGTGGAGACACCCCTTGTCAGAGCCGA CCCGTTTCAAGACCTTAGATTAGAATCCCCCACTCGGAGTCTAAGCATGGATGCCCCAAGGGGTGTGCAT ATTCAAGCTCACGCTGGGAAAATTGAGGCGCTTTCTCAAATGGATATTCTTTTTCATAGTAGTGATGGAA TGCTTGTGCTTGATGCTGAAACTGTGTGCTTACCCAAGCTGGTGCAGGGGACGTGGGGTCCCTCTGGCAG CTCACAGAGCCTCTACGAAATCTGTGTGTGTCCAGATGGGAAGCTGTACCTGTCTGTGGCCGGTGTGAGC ACCACGTGCCAGGAGCACAGCCACATCTGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220093 protein sequence
Red=Cloning site Green=Tags(s) MVREQYTTATEGICIERPENQYVYKIGIYGWRKRCLYLFVLLLLIILVVNLALTIWILKVMWFSPAGMGH LCVTKDGLRLEGESEFLFPLYAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQF QINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRSLSMDAPRGVH IQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVS TTCQEHSHICL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000231 |
ORF Size | 873 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000231.2, NP_000222.1 |
RefSeq Size | 1661 bp |
RefSeq ORF | 876 bp |
Locus ID | 6445 |
UniProt ID | Q13326 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis |
MW | 32.4 kDa |
Gene Summary | This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220093L3 | Lenti ORF clone of Human sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (SGCG), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC220093L4 | Lenti ORF clone of Human sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (SGCG), mGFP tagged |
CNY 5,890.00 |
|
RG220093 | SGCG (tGFP-tagged) - Human sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (SGCG) |
CNY 4,000.00 |
|
SC300030 | SGCG (untagged)-Human sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein) (SGCG) |
CNY 6,270.00 |
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