PCSK9 (NM_174936) Human Tagged Lenti ORF Clone
CAT#: RC220000L2
- LentiORF®
Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), mGFP tagged
"NM_174936" in other vectors (6)
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CNY 10,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | FH3; FHCL3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC220000).
|
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_174936 |
ORF Size | 2076 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_174936.2 |
RefSeq Size | 3636 bp |
RefSeq ORF | 2079 bp |
Locus ID | 255738 |
UniProt ID | Q8NBP7 |
Domains | Peptidase_S8 |
Protein Families | Secreted Protein |
MW | 71 kDa |
Gene Summary | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220000 | PCSK9 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 9 (PCSK9) |
CNY 7,600.00 |
|
RC220000L1 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), Myc-DDK-tagged |
CNY 10,000.00 |
|
RC220000L3 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), Myc-DDK-tagged |
CNY 10,000.00 |
|
RC220000L4 | Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), mGFP tagged |
CNY 10,000.00 |
|
RG220000 | PCSK9 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 9 (PCSK9) |
CNY 9,200.00 |
|
SC101299 | PCSK9 (untagged)-Human proprotein convertase subtilisin/kexin type 9 (PCSK9) |
CNY 7,616.00 |