United States
Germany
Japan
United Kingdom
China
RAD17 (NM_133341) Human Tagged ORF Clone
CAT＃: RC219964
RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4
ORF Plasmid: tGFP
"NM_133341" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,352.00
CNY 5,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCYC; HRAD17; R24L; RAD17SP; RAD24 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219964 representing NM_133341
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAG TTTTAGAAAGGCAACCAAAACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGAC AACGACCTTAAAAATACTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCA GACTTCCAAAAAGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGT CTCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGA TGATCTGAGAACTGATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCAT ACTTTACATGAAGTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACA GTCTCAGTGGAGATAATAATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAA TATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCT AACAAGAATGGAGGAAAAATTACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTG GTGATATCAGAAGTGCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAG GAAAAAAGGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGG GTTTTTGAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGG GGAAAATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATC AGAATATGAACGGGATACATTACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGAC TTATTTAATTTATATCTTCACCAAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCA GTGAATTTCTGAGTTTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAG CACATCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGA GGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGCC TGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAGCTATTGCCATA CCTTGCTCTACTAACCATTCCAATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGGCTC CCTCTGAAGCGACACTTTGGAAGATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATAGACC CTGACAGCGGAGATGAAGCCCAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCCACTCA AGCCACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCTAGCCAG CCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGTGATGGGA CA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219964 representing NM_133341
Red=Cloning site Green=Tags(s) MNQHELAVHKKKIEEVETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLP DFQKDDFKGMFNTESSFHMFPYQSQIAVFKEFLLRATKYNKLQMLGDDLRTDKKIILVEDLPNQFYRDSH TLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISFNPVAPTIMMKFLNRIVTIEA NKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSSKGENNLRPRKKGMSLKSDAVLSKSKRRKKPDR VFENQEVQAIGGKDVSLFLFRALGKILYCKRASLTELDSPRLPSHLSEYERDTLLVEPEEVVEMSHMPGD LFNLYLHQNYIDFFMEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRGVMHSNKARGYAHCQGG GSSFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCLQTQLLPYLALLTIPMRNQAQISFIQDIGRL PLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQATVPETWSLPLSQNSASELPASQ PQPFSAQGDMEENIIIEDYESDGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133341 |
| ORF Size | 1752 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133341.2, NP_579919.1 |
| RefSeq Size | 2592 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 5884 |
| UniProt ID | O75943 |
| Protein Families | Druggable Genome |
| MW | 66 kDa |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219964L3 | Lenti-ORF clone of RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 7,030.00 |
|
| RC219964L4 | Lenti-ORF clone of RAD17 (mGFP-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 7,030.00 |
|
| RG219964 | RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 5,610.00 |
|
| SC305944 | RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 9,500.00 |
