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Axin 1 (AXIN1) (NM_003502) Human Tagged ORF Clone
CAT＃: RC219592
AXIN1 (Myc-DDK-tagged)-Human axin 1 (AXIN1), transcript variant 1
ORF Plasmid: tGFP
"NM_003502" in other vectors (6)
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CNY 6,312.00
CNY 9,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AXIN; PPP1R49 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219592 representing NM_003502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATATCCAAGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCC CAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGG GAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTAT GAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGG ATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGCCGACTTGCTGGACTT CTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTG GCGAGAGCCATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCA CCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGTTTGACCAGGCCCAGAC CGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAATAT ACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCA TATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGA TGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTC TCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCT ATTATGTCAATGCCGGCTATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTC CAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCCATACAGGATCCGTAAG CAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCA CGTACCGGGTGCCGAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGA GGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCGCGTGCGCATGGAGGAG GAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTT GGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCC TGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGC CATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCGCCTCGGGGCACG GGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCA CCACGTCCACCACAGCACAGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGC AGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCG CTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAA GAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAG AAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGGACCGGCCACGGGTCTT CGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCC TCAGCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCT CCCAACCCCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAG CACCCTCCAAGCAGAGGTATGTGCAGGAGGTTATGCGGCGGGGACGCGCCTGCGTCAGGCCAGCGTGCGC GCCGGTGCTGCACGTGGTACCAGCCGTGTCGGACATGGAGCTCTCCGAGACAGAGACAAGATCGCAGAGG AAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTTGTGGCGTACTACTTCTGCGGGGAACCCA TCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAA GGGCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTT CGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219592 representing NM_003502
Red=Cloning site Green=Tags(s) MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGY EPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKL ARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEY TRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRV SSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRK QHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEE EGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPG HRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQS SFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQ KIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPA PNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQR KVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEV REDEAVLPVFEEKIIGKVEKVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003502 |
| ORF Size | 2586 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003502.4 |
| RefSeq Size | 3477 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 8312 |
| UniProt ID | O15169 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway |
| Protein Pathways | Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway |
| MW | 95.5 kDa |
| Gene Summary | This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219592L1 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 1, Myc-DDK-tagged |
CNY 8,712.00 |
|
| RC219592L2 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 1, mGFP tagged |
CNY 11,020.00 |
|
| RC219592L3 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 1, Myc-DDK-tagged |
CNY 11,020.00 |
|
| RC219592L4 | Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 1, mGFP tagged |
CNY 11,020.00 |
|
| RG219592 | AXIN1 (tGFP-tagged) - Human axin 1 (AXIN1), transcript variant 1 |
CNY 7,912.00 |
|
| SC303321 | AXIN1 (untagged)-Human axin 1 (AXIN1), transcript variant 1 |
CNY 6,320.00 |
