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VPS29 (NM_016226) Human Tagged ORF Clone
CAT＃: RC219311
- TrueORF®
VPS29 (Myc-DDK-tagged)-Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1
ORF Plasmid: tGFP
"NM_016226" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DC7; DC15; PEP11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219311 representing NM_016226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTGTTGGTATTAGGAGATCTGCACATCCCACACCGGTGCAACAGTTTGCCAGCTAAATTCAAAA AACTCCTGGTGCCAGGAAAAATTCAGCACATTCTCTGCACAGGAAACCTTTGCACCAAAGAGAGTTATGA CTATCTCAAGACTCTGGCTGGTGATGTTCATATTGTGAGAGGAGACTTCGATGAGAATCTGAATTATCCA GAACAGAAAGTTGTGACTGTTGGACAGTTCAAAATTGGTCTGATCCATGGACATCAAGTTATTCCATGGG GAGATATGGCCAGCTTAGCCCTGTTGCAGAGGCAATTTGATGTGGACATTCTTATCTCGGGACACACACA CAAATTTGAAGCATTTGAGCATGAAAATAAATTCTACATTAATCCAGGTTCTGCCACTGGGGCATATAAT GCCTTGGAAACAAACATTATTCCATCATTTGTGTTGATGGATATCCAGGCTTCTACAGTGGTCACCTATG TGTATCAGCTAATTGGAGATGATGTGAAAGTAGAACGAATCGAATACAAAAAACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219311 representing NM_016226
Red=Cloning site Green=Tags(s) MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYP EQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYN ALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016226 |
| ORF Size | 546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016226.5 |
| RefSeq Size | 1095 bp |
| RefSeq ORF | 549 bp |
| Locus ID | 51699 |
| UniProt ID | Q9UBQ0 |
| Domains | Metallophos |
| MW | 20.3 kDa |
| Gene Summary | This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219311L1 | Lenti-ORF clone of VPS29 (Myc-DDK-tagged)-Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1 |
CNY 4,800.00 |
|
| RC219311L2 | Lenti-ORF clone of VPS29 (mGFP-tagged)-Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1 |
CNY 5,890.00 |
|
| RC219311L3 | Lenti-ORF clone of VPS29 (Myc-DDK-tagged)-Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1 |
CNY 5,890.00 |
|
| RC219311L4 | Lenti-ORF clone of VPS29 (mGFP-tagged)-Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1 |
CNY 5,890.00 |
|
| RG219311 | VPS29 (tGFP-tagged) - Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1 |
CNY 4,370.00 |
|
| SC114392 | VPS29 (untagged)-Human vacuolar protein sorting 29 homolog (S. cerevisiae) (VPS29), transcript variant 1 |
CNY 2,400.00 |
