United States
Germany
Japan
United Kingdom
China
TRPC4 (NM_016179) Human Tagged ORF Clone
CAT＃: RC218941
TRPC4 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha
ORF Plasmid: tGFP
"NM_016179" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 10,728.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HTRP-4; HTRP4; TRP4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218941 representing NM_016179
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTCTATTACAAAAGAAATGTTAATGCTCCCTATAGAGACCGCATCCCTCTAAGGATAGTAA GAGCAGAATCAGAACTCTCGCCATCAGAAAAAGCCTACTTGAATGCTGTGGAAAAGGGAGATTATGCCAG TGTCAAGAAATCCCTAGAGGAAGCTGAAATTTATTTTAAAATCAATATTAATTGCATTGATCCTCTCGGA AGAACTGCTCTCCTCATTGCAATTGAAAATGAGAACTTGGAGCTCATCGAACTACTCTTAAGCTTTAATG TCTATGTTGGAGATGCTCTATTACATGCTATCAGAAAAGAAGTCGTCGGAGCTGTTGAGCTGTTATTGAA CCACAAAAAACCTAGTGGAGAAAAACAGGTGCCTCCTATACTCCTTGATAAGCAGTTCTCTGAATTCACT CCAGACATTACACCAATCATTTTGGCAGCCCATACAAATAATTATGAGATAATAAAACTCTTGGTTCAGA AAGGAGTCTCAGTGCCTCGACCCCACGAGGTCCGCTGTAACTGTGTGGAATGCGTGTCCAGTTCAGATGT GGACAGCCTCCGTCACTCACGCTCCAGACTCAACATCTACAAGGCCTTGGCCAGTCCCTCTCTCATTGCA CTGTCAAGCGAAGATCCTTTTCTCACAGCCTTTCAGTTAAGTTGGGAACTTCAGGAACTGAGCAAGGTGG AAAATGAATTCAAGTCGGAGTATGAAGAGCTGTCACGGCAGTGCAAACAATTTGCTAAGGACCTACTGGA TCAGACGAGAAGTTCCAGAGAACTGGAAATCATTCTTAATTACCGAGATGACAATAGTCTCATAGAAGAA CAAAGTGGAAATGATCTTGCAAGACTAAAATTGGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCCCAGC CCAATTGTCAACAGCTGCTGGCATCTCGCTGGTACGATGAGTTTCCAGGCTGGAGGAGAAGACACTGGGC AGTGAAGATGGTGACATGTTTCATAATAGGACTTCTTTTTCCTGTCTTCTCTGTGTGCTACCTGATAGCT CCCAAAAGCCCACTTGGACTGTTCATCAGGAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATT TGACTTTTTTGTTCCTGCTGCTGCTTGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC ACCACCAACCATCGTCGAGTGGATGATATTACCGTGGGTCCTGGGCTTCATATGGGGAGAAATTAAACAG ATGTGGGATGGCGGACTTCAGGACTACATCCATGATTGGTGGAATCTAATGGACTTTGTAATGAACTCCT TATATTTAGCAACAATCTCCTTGAAAATTGTTGCATTTGTAAAGTACAGTGCCCTTAATCCACGAGAATC ATGGGACATGTGGCATCCCACTCTGGTGGCAGAGGCTTTATTTGCTATTGCAAACATCTTCAGTTCTCTG CGTCTGATCTCACTGTTTACTGCAAATTCTCACCTGGGACCTCTGCAAATATCTCTGGGAAGAATGCTCC TGGACATTTTGAAGTTTCTATTCATATACTGCCTTGTGTTGCTAGCATTTGCAAATGGCCTAAATCAATT GTACTTCTATTATGAAGAAACGAAAGGGTTAACCTGCAAAGGCATAAGATGTGAAAAGCAGAATAATGCA TTTTCAACGTTATTTGAGACACTGCAGTCCCTGTTTTGGTCAATATTTGGGCTCATCAATTTATATGTGA CCAATGTCAAAGCACAGCATGAATTTACTGAGTTTGTTGGTGCCACCATGTTTGGGACATACAATGTCAT CTCTCTGGTTGTTCTACTCAACATGTTAATAGCTATGATGAATAATTCTTACCAACTGATTGCTGACCAT GCAGATATAGAATGGAAATTTGCACGAACAAAGCTTTGGATGAGTTATTTTGAAGAAGGAGGTACTCTGC CTACTCCCTTCAATGTCATCCCGAGCCCCAAGTCTCTCTGGTACCTGATCAAATGGATCTGGACACACTT GTGCAAGAAAAAGATGAGAAGAAAGCCAGAAAGTTTTGGAACAATAGGGAGGCGAGCTGCTGATAACTTG AGAAGACATCACCAATACCAAGAAGTTATGAGGAACCTGGTGAAGCGATACGTTGCTGCAATGATTAGAG ATGCTAAAACTGAAGAAGGCCTGACCGAAGAGAACTTTAAGGAACTAAAGCAAGACATTTCTAGTTTCCG CTTTGAAGTCCTGGGATTACTAAGAGGAAGCAAACTTTCCACAATACAATCTGCGAATGCCTCGAAGGAG TCTTCAAATTCGGCAGACTCAGATGAAAAGAGTGATAGCGAAGGTAATAGCAAGGACAAGAAAAAGAATT TCAGCCTTTTTGATTTAACCACCCTGATTCATCCGAGATCAGCAGCAATTGCCTCTGAAAGACATAACAT AAGCAATGGCTCTGCCCTGGTGGTTCAGGAGCCGCCCAGGGAGAAGCAGAGAAAAGTGAATTTTGTGACC GATATCAAAAACTTTGGGTTATTTCATAGACGATCAAAACAAAATGCTGCTGAGCAAAATGCAAACCAAA TCTTCTCTGTTTCAGAAGAAGTTGCTCGTCAACAGGCTGCAGGACCACTTGAGAGAAATATTCAACTGGA ATCTCGAGGATTAGCTTCACGGGGTGACCTGAGCATTCCCGGTCTCAGTGAACAATGTGTGTTAGTAGAC CATAGAGAAAGGAATACGGACACACTGGGGTTACAGGTAGGAAAGAGAGTGTGTCCATTCAAGTCAGAGA AGGTGGTGGTGGAGGACACGGTTCCTATAATACCAAAGGAGAAACATGCAAAAGAAGAGGACTCTAGTAT AGACTATGATCTAAACCTCCCAGACACAGTCACCCACGAAGATTACGTGACCACAAGATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218941 representing NM_016179
Red=Cloning site Green=Tags(s) MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGDYASVKKSLEEAEIYFKININCIDPLG RTALLIAIENENLELIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSEFT PDITPIILAAHTNNYEIIKLLVQKGVSVPRPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIA LSSEDPFLTAFQLSWELQELSKVENEFKSEYEELSRQCKQFAKDLLDQTRSSRELEIILNYRDDNSLIEE QSGNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAVKMVTCFIIGLLFPVFSVCYLIA PKSPLGLFIRKPFIKFICHTASYLTFLFLLLLASQHIDRSDLNRQGPPPTIVEWMILPWVLGFIWGEIKQ MWDGGLQDYIHDWWNLMDFVMNSLYLATISLKIVAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSL RLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNA FSTLFETLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADH ADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSPKSLWYLIKWIWTHLCKKKMRRKPESFGTIGRRAADNL RRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTEENFKELKQDISSFRFEVLGLLRGSKLSTIQSANASKE SSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVT DIKNFGLFHRRSKQNAAEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVD HRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016179 |
| ORF Size | 2931 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016179.4 |
| RefSeq Size | 3440 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 7223 |
| UniProt ID | Q9UBN4 |
| Domains | ANK, ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 111.9 kDa |
| Gene Summary | This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Englerin A Agonizes the TRPC4/C5 Cation Channels to Inhibit Tumor Cell Line Proliferation
,Carson, C;Raman, P;Tullai, J;Xu, L;Henault, M;Thomas, E;Yeola, S;Lao, J;McPate, M;Verkuyl, JM;Marsh, G;Sarber, J;Amaral, A;Bailey, S;Lubicka, D;Pham, H;Miranda, N;Ding, J;Tang, HM;Ju, H;Tranter, P;Ji, N;Krastel, P;Jain, RK;Schumacher, AM;Loureiro, JJ;Geor,
PLoS ONE
,PubMed ID 26098886
[TRPC4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218941L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged |
CNY 13,128.00 |
|
| RC218941L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged |
CNY 5,890.00 |
|
| RC218941L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, Myc-DDK-tagged |
CNY 13,128.00 |
|
| RC218941L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha, mGFP tagged |
CNY 13,128.00 |
|
| RG218941 | TRPC4 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha |
CNY 12,328.00 |
|
| SC114411 | TRPC4 (untagged)-Human transient receptor potential cation channel, subfamily C, member 4 (TRPC4), transcript variant alpha |
CNY 10,744.00 |
