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KCNQ2 (NM_172109) Human Tagged ORF Clone
CAT＃: RC218643
KCNQ2 (Myc-DDK-tagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 5
ORF Plasmid: tGFP
"NM_172109" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BFNC; DEE7; EBN; EBN1; ENB1; HNSPC; KCNA11; KV7.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218643 representing NM_172109
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGAAGTCGCGCAACGGCGGCGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCT TCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTGATCGCCGGCTCCGAGGC CCCCAAGCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGGGAAGCCCCCCAAGCGC AACGCCTTCTACCGCAAGCTGCAGAATTTCCTCTACAACGTGCTGGAGCGGCCGCGCGGCTGGGCGTTCA TCTACCACGCCTACGTGTTCCTCCTGGTTTTCTCCTGCCTCGTGCTGTCTGTGTTTTCCACCATCAAGGA GTATGAGAAGAGCTCGGAGGGGGCCCTCTACATCCTGGAAATCGTGACTATCGTGGTGTTTGGCGTGGAG TACTTCGTGCGGATCTGGGCCGCAGGCTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTG CCCGGAAACCGTTCTGTGTGATTGACATCATGGTGCTCATCGCCTCCATTGCGGTGCTGGCCGCCGGCTC CCAGGGCAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGATTCTGCGGATGATCCGC ATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGTCTATGCCCACAGCAAGGAGCTGGTCA CTGCCTGGTACATCGGCTTCCTTTGTCTCATCCTGGCCTCGTTCCTGGTGTACTTGGCAGAGAAGGGGGA GAACGACCACTTTGACACCTACGCGGATGCACTCTGGTGGGGCCTGATCACGCTGACCACCATTGGCTAC GGGGACAAGTACCCCCAGACCTGGAACGGCAGGCTCCTTGCGGCAACCTTCACCCTCATCGGTGTCTCCT TCTTCGCGCTGCCTGCAGGCATCTTGGGGTCTGGGTTTGCCCTGAAGGTTCAGGAGCAGCACAGGCAGAA GCACTTTGAGAAGAGGCGGAACCCGGCAGCAGGCCTGATCCAGTCGGCCTGGAGATTCTACGCCACCAAC CTCTCGCGCACAGACCTGCACTCCACGTGGCAGTACTACGAGCGAACGGTCACCGTGCCCATGTACAGGT ACCGCCGCCGGGCACCTGCCACCAAGCAACTGTTTCATTTTTTATTTTCCATTTGTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218643 representing NM_172109
Red=Cloning site Green=Tags(s) MVQKSRNGGVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALLIAGSEAPKRGSILSKPRAGGAGAGKPPKR NAFYRKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLVLSVFSTIKEYEKSSEGALYILEIVTIVVFGVE YFVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIMVLIASIAVLAAGSQGNVFATSALRSLRFLQILRMIR MDRRGGTWKLLGSVVYAHSKELVTAWYIGFLCLILASFLVYLAEKGENDHFDTYADALWWGLITLTTIGY GDKYPQTWNGRLLAATFTLIGVSFFALPAGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQSAWRFYATN LSRTDLHSTWQYYERTVTVPMYRYRRRAPATKQLFHFLFSICS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172109 |
| ORF Size | 1179 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172109.3 |
| RefSeq Size | 1425 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 3785 |
| UniProt ID | O43526 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 44.1 kDa |
| Gene Summary | The M channel is a slowly activating and deactivating potassium channel that plays a critical role in the regulation of neuronal excitability. The M channel is formed by the association of the protein encoded by this gene and a related protein encoded by the KCNQ3 gene, both integral membrane proteins. M channel currents are inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 1 (BFNC), also known as epilepsy, benign neonatal type 1 (EBN1). At least five transcript variants encoding five different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218643L3 | Lenti ORF clone of Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 5, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218643L4 | Lenti ORF clone of Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 5, mGFP tagged |
CNY 5,890.00 |
|
| RG218643 | KCNQ2 (tGFP-tagged) - Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 5 |
CNY 4,370.00 |
|
| SC122047 | KCNQ2 (untagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 5 |
CNY 3,656.00 |
