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LNK (SH2B3) (NM_005475) Human Tagged ORF Clone
CAT＃: RC218359
SH2B3 (Myc-DDK-tagged)-Human SH2B adaptor protein 3 (SH2B3)
ORF Plasmid: tGFP
"NM_005475" in other vectors (6)
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CNY 4,280.00
CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IDDM20; LNK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218359 representing NM_005475
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGGCCTGCCCTGCAGCCCTCCTCGCCCTCTTCCGCGCCCTCAGCCTCCCCGGCGGCGGCCCCGC GGGGCTGGAGCGAGTTCTGTGAGTTGCACGCCGTAGCGGCGGCCCGGGAGCTGGCCCGCCAGTACTGGCT GTTCGCCCGGGAGCATCCGCAGCACGCGCCGCTGCGCGCCGAGCTGGTGTCGCTGCAGTTCACCGACCTC TTCCAGCGCTACTTCTGCCGCGAGGTGCGCGACGGACGGGCGCCGGGCCGCGACTACCGGGACACAGGCC GTGGGCCCCCAGCCAAGGCCGAGGCGTCCCCGGAGCCAGGCCCCGGCCCCGCCGCCCCTGGCCTGCCCAA GGCCCGCAGCTCTGAGGAGCTGGCCCCGCCGCGGCCGCCCGGGCCCTGCTCCTTCCAGCACTTTCGCCGC AGCCTCCGCCACATCTTCCGCCGCCGCTCGGCCGGGGAGCTGCCAGCGGCCCACACCGCTGCCGCCCCCG GGACCCCCGGAGAGGCTGCTGAGACCCCCGCCCGGCCTGGCCTGGCCAAGAAGTTCCTGCCCTGGAGCCT GGCCCGGGAGCCGCCACCCGAGGCGCTGAAGGAGGCGGTGCTGCGCTACAGCCTGGCCGACGAGGCCTCC ATGGACAGCGGGGCACGCTGGCAGCGCGGGAGGCTGGCGCTGCGCCGGGCCCCGGGCCCCGATGGCCCCG ACCGCGTGCTGGAGCTCTTCGACCCACCCAAGAGTTCAAGGCCCAAGCTACAAGCAGCTTGCTCCAGCAT CCAGGAGGTCCGGCGGTGCACACGGCTTGAGATGCCTGACAACCTTTACACCTTTGTGCTGAAGGTGAAG GACCGGACAGACATCATCTTTGAGGTGGGAGACGAGCAGCAGCTGAATTCATGGATGGCTGAGCTCTCGG AGTGCACAGGCCGAGGGCTGGAGAGCACAGAAGCAGAGATGCATATTCCCTCAGCCCTAGAGCCTAGCAC GTCCAGCTCCCCAAGGGGCAGCACAGATTCCCTTAACCAAGGTGCTTCTCCTGGGGGGCTGCTGGACCCG GCCTGCCAGAAGACGGACCATTTCCTGTCCTGCTACCCCTGGTTCCACGGCCCCATCTCCAGAGTGAAAG CAGCTCAGCTGGTTCAGCTGCAGGGCCCTGATGCTCATGGAGTGTTCCTGGTGCGGCAGAGCGAGACGCG GCGTGGGGAATACGTGCTCACTTTCAACTTTCAGGGGATAGCCAAGCACCTGCGCCTGTCGCTGACAGAG CGGGGCCAGTGCCGTGTGCAGCACCTCCACTTTCCCTCGGTCGTGGACATGCTCCACCACTTCCAGCGCT CGCCCATCCCACTCGAGTGCGGCGCCGCCTGTGATGTCCGGCTCTCCAGCTACGTGGTAGTCGTCTCCCA ACCACCAGGTTCCTGCAACACGGTCCTCTTCCCTTTCTCCCTTCCTCACTGGGATTCAGAGTCCCTTCCT CACTGGGGTTCAGAGTTGGGCCTTCCCCACCTTAGTTCTTCTGGCTGTCCCCGGGGGCTCAGCCCAGAGG GTCTCCCAGGGCGATCCTCACCCCCCGAGCAGATCTTCCACCTGGTGCCTTCGCCCGAAGAACTGGCCAA CAGCCTGCAGCACCTGGAGCATGAGCCTGTGAATCGAGCCCGGGACTCGGACTACGAAATGGACTCATCC TCCCGGAGCCACCTGCGGGCCATAGACAATCAGTACACACCTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218359 representing NM_005475
Red=Cloning site Green=Tags(s) MNGPALQPSSPSSAPSASPAAAPRGWSEFCELHAVAAARELARQYWLFAREHPQHAPLRAELVSLQFTDL FQRYFCREVRDGRAPGRDYRDTGRGPPAKAEASPEPGPGPAAPGLPKARSSEELAPPRPPGPCSFQHFRR SLRHIFRRRSAGELPAAHTAAAPGTPGEAAETPARPGLAKKFLPWSLAREPPPEALKEAVLRYSLADEAS MDSGARWQRGRLALRRAPGPDGPDRVLELFDPPKSSRPKLQAACSSIQEVRRCTRLEMPDNLYTFVLKVK DRTDIIFEVGDEQQLNSWMAELSECTGRGLESTEAEMHIPSALEPSTSSSPRGSTDSLNQGASPGGLLDP ACQKTDHFLSCYPWFHGPISRVKAAQLVQLQGPDAHGVFLVRQSETRRGEYVLTFNFQGIAKHLRLSLTE RGQCRVQHLHFPSVVDMLHHFQRSPIPLECGAACDVRLSSYVVVVSQPPGSCNTVLFPFSLPHWDSESLP HWGSELGLPHLSSSGCPRGLSPEGLPGRSSPPEQIFHLVPSPEELANSLQHLEHEPVNRARDSDYEMDSS SRSHLRAIDNQYTPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005475 |
| ORF Size | 1725 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005475.3 |
| RefSeq Size | 5423 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 10019 |
| UniProt ID | Q9UQQ2 |
| Domains | SH2, PH |
| Protein Pathways | Neurotrophin signaling pathway |
| MW | 63 kDa |
| Gene Summary | This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218359L1 | Lenti ORF clone of Human SH2B adaptor protein 3 (SH2B3), Myc-DDK-tagged |
CNY 6,680.00 |
|
| RC218359L2 | Lenti ORF clone of Human SH2B adaptor protein 3 (SH2B3), mGFP tagged |
CNY 6,460.00 |
|
| RC218359L3 | Lenti ORF clone of Human SH2B adaptor protein 3 (SH2B3), Myc-DDK-tagged |
CNY 6,680.00 |
|
| RC218359L4 | Lenti ORF clone of Human SH2B adaptor protein 3 (SH2B3), mGFP tagged |
CNY 6,680.00 |
|
| RG218359 | SH2B3 (tGFP-tagged) - Human SH2B adaptor protein 3 (SH2B3) |
CNY 5,880.00 |
|
| SC116715 | SH2B3 (untagged)-Human SH2B adaptor protein 3 (SH2B3) |
CNY 4,288.00 |
