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Metabotropic Glutamate Receptor 2 (GRM2) (NM_000839) Human Tagged ORF Clone
CAT＃: RC218103
- TrueORF®
GRM2 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1
ORF Plasmid: tGFP
"NM_000839" in other vectors (6)
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CNY 6,384.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GLUR2; GPRC1B; mGlu2; MGLUR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218103 representing NM_000839.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGATCGCTGCTTGCGCTCCTGGCACTGCTGCTGCTGTGGGGTGCTGTGGCTGAGGGCCCAGCCAAG AAGGTGCTGACCCTGGAGGGAGACTTGGTGCTGGGTGGGCTGTTCCCAGTGCACCAGAAGGGCGGCCCA GCAGAGGACTGTGGTCCTGTCAATGAGCACCGTGGCATCCAGCGCCTGGAGGCCATGCTTTTTGCACTG GACCGCATCAACCGTGACCCGCACCTGCTGCCTGGCGTGCGCCTGGGTGCACACATCCTCGACAGTTGC TCCAAGGACACACATGCGCTGGAGCAGGCACTGGACTTTGTGCGTGCCTCACTCAGCCGTGGTGCTGAT GGCTCACGCCACATCTGCCCCGACGGCTCTTATGCGACCCATGGTGATGCTCCCACTGCCATCACTGGT GTTATTGGCGGTTCCTACAGTGATGTCTCCATCCAGGTGGCCAACCTCTTGAGGCTATTTCAGATCCCA CAGATTAGCTACGCCTCTACCAGTGCCAAGCTGAGTGACAAGTCCCGCTATGACTACTTTGCCCGCACA GTGCCTCCTGACTTCTTCCAAGCCAAGGCCATGGCTGAGATTCTCCGCTTCTTCAACTGGACCTATGTG TCCACTGTGGCGTCTGAGGGCGACTATGGCGAGACAGGCATTGAGGCCTTTGAGCTAGAGGCTCGTGCC CGCAACATCTGTGTGGCCACCTCGGAGAAAGTGGGCCGTGCCATGAGCCGCGCGGCCTTTGAGGGTGTG GTGCGAGCCCTGCTGCAGAAGCCCAGTGCCCGCGTGGCTGTCCTGTTCACCCGTTCTGAGGATGCCCGG GAGCTGCTTGCTGCCAGCCAGCGCCTCAATGCCAGCTTCACCTGGGTGGCCAGTGATGGTTGGGGGGCC CTGGAGAGTGTGGTGGCAGGCAGTGAGGGGGCTGCTGAGGGTGCTATCACCATCGAGCTGGCCTCCTAC CCCATCAGTGACTTTGCCTCCTACTTCCAGAGCCTGGACCCTTGGAACAACAGCCGGAACCCCTGGTTC CGTGAATTCTGGGAGCAGAGGTTCCGCTGCAGCTTCCGGCAGCGAGACTGCGCAGCCCACTCTCTCCGG GCTGTGCCCTTTGAGCAGGAGTCCAAGATCATGTTTGTGGTCAATGCAGTGTACGCCATGGCCCATGCG CTCCACAACATGCACCGTGCCCTCTGCCCCAACACCACCCGGCTCTGTGACGCGATGCGGCCAGTTAAC GGGCGCCGCCTCTACAAGGACTTTGTGCTCAACGTCAAGTTTGATGCCCCCTTTCGCCCAGCTGACACC CACAATGAGGTCCGCTTTGACCGCTTTGGTGATGGTATTGGCCGCTACAACATCTTCACCTATCTGCGT GCAGGCAGTGGGCGCTATCGCTACCAGAAGGTGGGCTACTGGGCAGAAGGCTTGACTCTGGACACCAGC CTCATCCCATGGGCCTCACCCTCAGCCGGCCCCCTGCCCGCCTCTCGCTGCAGTGAGCCCTGCCTCCAG AATGAGGTGAAGAGTGTGCAGCCGGGCGAAGTCTGCTGCTGGCTCTGCATTCCGTGCCAGCCCTATGAG TACCGATTGGACGAATTCACTTGCGCTGATTGTGGCCTGGGCTACTGGCCCAATGCCAGCCTGACTGGC TGCTTCGAACTGCCCCAGGAGTACATCCGCTGGGGCGATGCCTGGGCTGTGGGACCTGTCACCATCGCC TGCCTCGGTGCCCTGGCCACCCTCTTTGTGCTGGGTGTCTTTGTGCGGCACAATGCCACACCAGTGGTC AAGGCCTCAGGTCGGGAGCTCTGCTACATCCTGCTGGGTGGTGTCTTCCTCTGCTACTGCATGACCTTC ATCTTCATTGCCAAGCCATCCACGGCAGTGTGTACCTTACGGCGTCTTGGTTTGGGCACTGCCTTCTCT GTCTGCTACTCAGCCCTGCTCACCAAGACCAACCGCATTGCACGCATCTTCGGTGGGGCCCGGGAGGGT GCCCAGCGGCCACGCTTCATCAGTCCTGCCTCACAGGTGGCCATCTGCCTGGCACTTATCTCGGGCCAG CTGCTCATCGTGGTCGCCTGGCTGGTGGTGGAGGCACCGGGCACAGGCAAGGAGACAGCCCCCGAACGG CGGGAGGTGGTGACACTGCGCTGCAACCACCGCGATGCAAGTATGTTGGGCTCGCTGGCCTACAATGTG CTCCTCATCGCGCTCTGCACGCTTTATGCCTTCAAGACTCGCAAGTGCCCCGAAAACTTCAACGAGGCC AAGTTCATTGGCTTCACCATGTACACCACCTGCATCATCTGGCTGGCATTCCTGCCCATCTTCTATGTC ACCTCCAGTGACTACCGGGTACAGACCACCACCATGTGCGTGTCAGTCAGCCTCAGCGGCTCCGTGGTG CTTGGCTGCCTCTTTGCGCCCAAGCTGCACATCATCCTCTTCCAGCCGCAGAAGAACGTGGTTAGCCAC CGGGCACCCACCAGCCGCTTTGGCAGTGCTGCTGCCAGGGCCAGCTCCAGCCTTGGCCAAGGGTCTGGC TCCCAGTTTGTCCCCACTGTTTGCAATGGCCGTGAGGTGGTGGACTCGACAACGTCATCGCTT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT >Peptide sequence encoded by RC218103
Blue=ORF Red=Cloning site Green=Tag(s) MGSLLALLALLLLWGAVAEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEDCGPVNEHRGIQRLEAMLFAL DRINRDPHLLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHGDAPTAITG VIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEILRFFNWTYV STVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALLQKPSARVAVLFTRSEDAR ELLAASQRLNASFTWVASDGWGALESVVAGSEGAAEGAITIELASYPISDFASYFQSLDPWNNSRNPWF REFWEQRFRCSFRQRDCAAHSLRAVPFEQESKIMFVVNAVYAMAHALHNMHRALCPNTTRLCDAMRPVN GRRLYKDFVLNVKFDAPFRPADTHNEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTS LIPWASPSAGPLPASRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTG CFELPQEYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYCMTF IFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPASQVAICLALISGQ LLIVVAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLLIALCTLYAFKTRKCPENFNEA KFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVSVSLSGSVVLGCLFAPKLHIILFQPQKNVVSH RAPTSRFGSAAARASSSLGQGSGSQFVPTVCNGREVVDSTTSSL TRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000839 |
| ORF Size | 2616 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000839.5 |
| RefSeq Size | 3186 bp |
| RefSeq ORF | 2619 bp |
| Locus ID | 2912 |
| UniProt ID | Q14416 |
| Domains | 7tm_3, ANF_receptor |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 95.6 kDa |
| Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cholesterol Localization Around the Metabotropic Glutamate Receptor 2
,Kurth, M;Lolicato, F;Sandoval-Perez, A;Amaya-Espinosa, H;Teslenko, A;Sinning, I;Beck, R;Brügger, B;Aponte-Santamaría, CA;,
J Phys Chem B
,PubMed ID 32954729
[Metabotropic Glutamate Receptor 2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218103L1 | Lenti ORF clone of Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1, Myc-DDK-tagged |
CNY 8,784.00 |
|
| RC218103L2 | Lenti ORF clone of Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC218103L3 | Lenti ORF clone of Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218103L4 | Lenti ORF clone of Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG218103 | GRM2 (tGFP-tagged) - Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1 |
CNY 7,984.00 |
|
| SC119644 | GRM2 (untagged)-Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1 |
CNY 6,392.00 |
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