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PDPR (NM_017990) Human Tagged ORF Clone
CAT＃: RC218100
PDPR (Myc-DDK-tagged)-Human pyruvate dehydrogenase phosphatase regulatory subunit (PDPR)
ORF Plasmid: tGFP
"NM_017990" in other vectors (4)
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CNY 9,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PDP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218100 representing NM_017990
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTTCTACCGGTTGCTGTCGATTGTTGGAAGACAAAGAGCCAGCCCAGGATGGCAGAACTGGTCCT CTGCAAGAAACAGCACGTCAGCTGCCGAGGCGCGTTCCATGGCCCTGCCCACCCAGGCACAGGTGGTCAT CTGTGGAGGTGGAATCACGGGCACTTCTGTGGCCTATCACCTCTCCAAAATGGGGTGGAAGGATATTGTC CTTTTGGAGCAGGGCAGGCTGGCTGCTGGCTCTACCAGGTTCTGTGCTGGCATCCTGAGCACTGCCAGGC ACTTGACCATTGAGCAGAAGATGGCAGACTACTCAAACAAACTCTACTATCAGTTAGAGCAAGAAACAGG GATCCAAACAGGTTACACAAGGACAGGCTCAATCTTTCTGGCCCAAACTCAGGACCGACTGATCTCCCTG AAGCGCATCAACGCAGGGCTGAATGTTATAGGTATCCCTTCTGAGATCATCTCCCCCAAGAAAGTGGCCG AGCTTCACCATCTCCTCAACGTGCACGACCTGGTGGGGGCCATGCATGTTCCCGAGGATGCAGTGGTGTC TTCCGCTGACGTGGCTCTTGCCCTGGCAAGTGCTGCCTCCCAAAATGGTGTTCAGATCTATGACCGGACA TCTGTTCTTCATGTAATGGTCAAAAAAGGTCAAGTTACTGGAGTGGAGACCGATAAAGGACAGATTGAAT GCCAGTATTTTGTCAACTGTGCTGGCCAGTGGGCATACGAGCTGGGTCTGTCCAACGAGGAGCCGGTTAG TATCCCGCTACATGCCTGCGAACACTTCTACCTCCTGACTCGCCCCTTGGAGACCCCTCTGCAGAGCAGC ACACCAACTATTGTGGATGCTGATGGAAGAATTTATATTCGGAACTGGCAGGGTGGCATCCTGTCTGGGG GCTTTGAGAAGAACCCGAAACCAATTTTCACTGAGGGCAAGAACCAGCTGGAGATTCAGAATCTACAGGA AGACTGGGATCACTTTGAGCCTCTGTTGAGTTCCCTTCTGAGGAGGATGCCAGAATTAGAGACTCTGGAG ATCATGAAGTTGGTGAACTGCCCAGAGACCTTCACACCAGACATGAGGTGCATCATGGGCGAGTCTCCTG CAGTGCAGGGCTACTTTGTCCTGGCAGGAATGAACTCTGCTGGCCTTTCATTTGGTGGAGGAGCCGGAAA GTACCTTGCCGAATGGATGGTACATGGTTATCCCTCAGAAAACGTTTGGGAATTGGACCTGAAACGTTTT GGAGCCCTCCAGAGCAGCCGCACCTTTCTGCGCCACCGGGTCATGGAAGTCATGCCTTTGATGTATGATC TGAAGGTTCCCCGCTGGGACTTCCAGACCGGTAGGCAGTTACGCACCTCTCCTCTCTACGACCGGCTGGA TGCACAGGGAGCCAGGTGGATGGAGAAACATGGATTTGAGAGGCCAAAGTACTTTGTTCCCCCCGACAAG GACCTCCTGGCATTGGAGCAGAGCAAGACTTTCTATAAGCCAGATTGGTTTGACATCGTGGAGTCTGAAG TCAAGTGCTGTAAGGAAGCTGTGTGTGTCATTGACATGTCCTCTTTCACAAAGTTTGAGATAACATCCAC TGGGGATCAGGCATTAGAAGTTCTACAGTACCTCTTCTCCAATGACCTGGATGTGCCTGTGGGCCACATT GTGCATACTGGCATGCTCAACGAGGGTGGAGGGTATGAAAATGACTGCAGCATAGCACGACTGAACAAGC GCAGTTTCTTCATGATCTCTCCAACCGACCAGCAGGTCCACTGTTGGGCCTGGCTTAAGAAACACATGCC GAAAGACAGCAACCTGCTCCTGGAGGACGTCACCTGGAAGTACACAGCCCTCAATCTGATTGGCCCTCGA GCTGTGGATGTGCTGTCTGAGTTGTCCTATGCCCCTATGACTCCAGACCACTTCCCAAGCCTCTTTTGCA AGGAGATGAGTGTGGGCTATGCAAATGGGATCCGGGTGATGAGCATGACGCACACAGGAGAGCCAGGATT CATGCTCTACATCCCCATAGAGTACGCCCTGCATGTATACAATGAAGTGATGAGTGTTGGCCAGAAATAC GGAATCCGGAATGCTGGGTATTACGCTCTTCGCAGTCTCCGAATTGAGAAGTTTTTTGCCTTCTGGGGTC AGGATATAAATAACCTCACCACGCCCCTGGAATGTGGACGAGAGTCTCGGGTGAAATTAGAGAAGGGCAT GGATTTCATTGGTCGCGACGCCCTCCTGCAGCAGAAGCAGAATGGAGTGTATAAACGCCTCACCATGTTC ATCCTGGACGACCATGATTCAGACCTAGACCTTTGGCCTTGGTGGGGAGAGCCCATTTACCGGAATGGGC AGTATGTTGGCAAGACCACCAGCAGTGCCTACAGCTACAGCCTGGAGCGCCACGTTTGCCTGGGCTTTGT GCACAATTTTTCTGAGGACACGGGGGAAGAGCAAGTGGTGACAGCAGATTTCATCAACCGGGGAGAGTAT GAGATTGACATCGCGGGATACCGCTTCCAGGCCAAGGCCAAGCTCTACCCTGTCGCCTCCCTCTTCACCC AGAAGCGCCGAAAGGATGACATGGAGCTGAGTGACTTACATGGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218100 representing NM_017990
Red=Cloning site Green=Tags(s) MMFYRLLSIVGRQRASPGWQNWSSARNSTSAAEARSMALPTQAQVVICGGGITGTSVAYHLSKMGWKDIV LLEQGRLAAGSTRFCAGILSTARHLTIEQKMADYSNKLYYQLEQETGIQTGYTRTGSIFLAQTQDRLISL KRINAGLNVIGIPSEIISPKKVAELHHLLNVHDLVGAMHVPEDAVVSSADVALALASAASQNGVQIYDRT SVLHVMVKKGQVTGVETDKGQIECQYFVNCAGQWAYELGLSNEEPVSIPLHACEHFYLLTRPLETPLQSS TPTIVDADGRIYIRNWQGGILSGGFEKNPKPIFTEGKNQLEIQNLQEDWDHFEPLLSSLLRRMPELETLE IMKLVNCPETFTPDMRCIMGESPAVQGYFVLAGMNSAGLSFGGGAGKYLAEWMVHGYPSENVWELDLKRF GALQSSRTFLRHRVMEVMPLMYDLKVPRWDFQTGRQLRTSPLYDRLDAQGARWMEKHGFERPKYFVPPDK DLLALEQSKTFYKPDWFDIVESEVKCCKEAVCVIDMSSFTKFEITSTGDQALEVLQYLFSNDLDVPVGHI VHTGMLNEGGGYENDCSIARLNKRSFFMISPTDQQVHCWAWLKKHMPKDSNLLLEDVTWKYTALNLIGPR AVDVLSELSYAPMTPDHFPSLFCKEMSVGYANGIRVMSMTHTGEPGFMLYIPIEYALHVYNEVMSVGQKY GIRNAGYYALRSLRIEKFFAFWGQDINNLTTPLECGRESRVKLEKGMDFIGRDALLQQKQNGVYKRLTMF ILDDHDSDLDLWPWWGEPIYRNGQYVGKTTSSAYSYSLERHVCLGFVHNFSEDTGEEQVVTADFINRGEY EIDIAGYRFQAKAKLYPVASLFTQKRRKDDMELSDLHGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017990 |
| ORF Size | 2637 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017990.5 |
| RefSeq Size | 7999 bp |
| RefSeq ORF | 2640 bp |
| Locus ID | 55066 |
| UniProt ID | Q8NCN5 |
| Domains | GCV_T |
| Protein Families | Druggable Genome |
| MW | 99.4 kDa |
| Gene Summary | Pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate and links glycolysis to the tricarboxylic acid cycle and fatty acid synthesis. The dephosphorylation and reactivation of PDC is catalyzed by pyruvate dehydrogenase phosphatase (PDP). The dimeric PDP has a catalytic subunit and a regulatory subunit. This gene encodes the FAD-containing regulatory subunit of PDP. The encoded protein acts to decrease the sensitivity of the PDP catalytic subunit to magnesium ions. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218100L3 | Lenti-ORF clone of PDPR (Myc-DDK-tagged)-Human pyruvate dehydrogenase phosphatase regulatory subunit (PDPR) |
CNY 9,030.00 |
|
| RC218100L4 | Lenti-ORF clone of PDPR (mGFP-tagged)-Human pyruvate dehydrogenase phosphatase regulatory subunit (PDPR) |
CNY 9,030.00 |
|
| RG218100 | PDPR (tGFP-tagged) - Human pyruvate dehydrogenase phosphatase regulatory subunit (PDPR) |
CNY 7,890.00 |
|
| SC314855 | PDPR (untagged)-Human pyruvate dehydrogenase phosphatase regulatory subunit (PDPR) |
CNY 10,312.00 |
