RTN3 (NM_006054) Human Tagged ORF Clone
CAT#: RC217819
RTN3 (Myc-DDK-tagged)-Human reticulon 3 (RTN3), transcript variant 1
ORF Plasmid: tGFP
"NM_006054" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ASYIP; HAP; NSPL2; NSPLII; RTN3-A1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217819 representing NM_006054
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCGTCGGCGGCCACTCAGTCCCATTCCATCTCCTCGTCGTCCTTCGGAGCCGAGCCGTCCG CGCCCGGCGGCGGCGGGAGCCCAGGAGCCTGCCCCGCCCTGGGGACGAAGAGCTGCAGCTCCTCCTGTGC GGTGCACGATCTGATTTTCTGGAGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACGCTGATCATG CTGCTTTCCCTGGCAGCTTTCAGTGTCATCAGTGTGGTTTCTTACCTCATCCTGGCTCTTCTCTCTGTCA CCATCAGCTTCAGGATCTACAAGTCCGTCATCCAAGCTGTACAGAAGTCAGAAGAAGGCCATCCATTCAA AGCCTACCTGGACGTAGACATTACTCTGTCCTCAGAAGCTTTCCATAATTACATGAATGCTGCCATGGTG CACATCAACAGGGCCCTGAAACTCATTATTCGTCTCTTTCTGGTAGAAGATCTGGTTGACTCCTTGAAGC TGGCTGTCTTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAACGGAATCACCCTTCTAATTCTTGC TGAACTGCTCATTTTCAGTGTCCCGATTGTCTATGAGAAGTACAAGACCCAGATTGATCACTATGTTGGC ATCGCCCGAGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAACTCCCTGGAATCGCCAAAAAAA AGGCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217819 representing NM_006054
Red=Cloning site Green=Tags(s) MAEPSAATQSHSISSSSFGAEPSAPGGGGSPGACPALGTKSCSSSCAVHDLIFWRDVKKTGFVFGTTLIM LLSLAAFSVISVVSYLILALLSVTISFRIYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHNYMNAAMV HINRALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLIFSVPIVYEKYKTQIDHYVG IARDQTKSIVEKIQAKLPGIAKKKAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006054 |
ORF Size | 708 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006054.4 |
RefSeq Size | 2583 bp |
RefSeq ORF | 711 bp |
Locus ID | 10313 |
UniProt ID | O95197 |
Domains | Reticulon |
Protein Families | Transmembrane |
MW | 25.4 kDa |
Gene Summary | This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12. [provided by RefSeq, May 2012] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The N-Degron Pathway Mediates ER-phagy
,Ji, C;Kim, H;Heo, A;Lee, S;Lee, M;Kim, S;Srinivasrao, G;Mun, S;Cha-Molstad, H;Ciechanover, A;Choi, C;Lee, H;Kim, B;Kwon, Y;,
Molecular Cell
,PubMed ID 31375263
[RTN3]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217819L1 | Lenti ORF clone of Human reticulon 3 (RTN3), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC217819L2 | Lenti ORF clone of Human reticulon 3 (RTN3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC217819L3 | Lenti ORF clone of Human reticulon 3 (RTN3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC217819L4 | Lenti ORF clone of Human reticulon 3 (RTN3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG217819 | RTN3 (tGFP-tagged) - Human reticulon 3 (RTN3), transcript variant 1 |
CNY 4,370.00 |
|
SC108563 | RTN3 (untagged)-Human reticulon 3 (RTN3), transcript variant 1 |
CNY 2,400.00 |