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TDRKH (NM_001083964) Human Tagged ORF Clone
CAT＃: RC217780
TDRKH (Myc-DDK-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4
ORF Plasmid: tGFP
"NM_001083964" in other vectors (4)
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CNY 3,840.00
CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TDRD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217780 representing NM_001083964
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACTGAACGGACTTCTTGGACAAGCCTGTCCACCATTCAGAAAATAGCCCTGGGCCTTGGGATCC CAGCCAGTGCAACAGTTGCCTATATCCTATACCGCAGGTATAGGGAAAGCAGAGAAGAGCGGCTGACATT TGTTGGGGAAGATGACATTGAGATAGAGATGCGGGTTCCCCAGGAGGCTGTGAAACTCATCATTGGCCGG CAAGGAGCCAATATTAAACAGCTGCGGAAACAGACAGGTGCTCGGATTGATGTGGACACAGAGGATGTAG GCGATGAGCGAGTGCTGCTTATCAGTGGTTTTCCTGTTCAGGCATCTGGAGCCAAAATTACCTGTGACAA AGAATCAGAAGGGACATTACTACTATCAAGACTTATAAAAATCTCAGGAACACAGAAGGAAGTGGCAGCA GCCAAGCATTTGATACTGGAGAAAGTTTCAGAAGATGAAGAACTTCGGAAGAGAATTGCTCATTCTGCAG AAACCAGGGTCCCACGCAAACAGCCAATCAGTGTGAGAAGAGAAGACATGACAGAGCCAGGTGGAGCTGG AGAGCCAGCATTATGGAAAAACACCAGTTCTAGCATGGAGCCGACTGCACCCCTGGTGACTCCTCCACCC AAAGGAGGAGGCGACATGGCTGTGGTAGTGTCAAAGGAAGGTTCCTGGGAGAAACCTAGTGATGACAGCT TTCAGAAGTCTGAAGCCCAGGCCATCCCAGAGATGCCCATGTTTGAAATCCCCAGTCCTGACTTCAGTTT TCATGCTGATGAGTACCTAGAAGTCTACGTTTCTGCTTCTGAGCACCCTAACCACTTCTGGATCCAGATC GTTGGCTCCCGCAGCCTGCAATTGGATAAGCTTGTCAATGAGATGACCCAGCACTATGAGAATAGTGTGC CTGAAGACTTGACTGTGCATGTAGGAGACATTGTAGCAGCACCTTTACCTACAAATGGTTCCTGGTATCG AGCCCGGGTCCTCGGCACCTTGGAGAATGGGAACTTGGACCTCTATTTTGTTGACTTTGGAGATAATGGA GATTGCCCACTGAAGGACCTCAGGGCTCTCAGGAGTGACTTCCTAAGCCTTCCATTTCAAGCAATAGAAT GTAGTCTGGCACGGATTGCTCCCTCAGGTGACCAGTGGGAAGAGGAAGCTTTGGATGAGTTTGATAGACT CACTCATTGTGCTGACTGGAAGCCTCTGGTAGCCAAGATCTCTAGCTATGTCCAGACTGGGATCTCAACT TGGCCAAAGATCTACTTATATGATACTAGCAATGGGAAGAAACTTGATATTGGGCTAGAATTAGTACACA AAGGATACGCAATTGAGCTTCCTGAAGACATAGAAGAAAACAGAGCTGTCCCAGACATGTTGAAGGACAT GGCCACAGAAACAGATGCCTCTCTCAGCACGTTGCTCACTGAGACCAAAAAGAGCTCTGGAGAGATAACA CATACCCTGTCCTGCCTCAGCTTATCAGAAGCTGCTTCCATGTCTGGTGATGATAACCTTGAAGATGACT ACTTACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217780 representing NM_001083964
Red=Cloning site Green=Tags(s) MSTERTSWTSLSTIQKIALGLGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGR QGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQASGAKITCDKESEGTLLLSRLIKISGTQKEVAA AKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPP KGGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQI VGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRARVLGTLENGNLDLYFVDFGDNG DCPLKDLRALRSDFLSLPFQAIECSLARIAPSGDQWEEEALDEFDRLTHCADWKPLVAKISSYVQTGIST WPKIYLYDTSNGKKLDIGLELVHKGYAIELPEDIEENRAVPDMLKDMATETDASLSTLLTETKKSSGEIT HTLSCLSLSEAASMSGDDNLEDDYLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001083964 |
| ORF Size | 1548 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083964.1, NP_001077433.1 |
| RefSeq Size | 2719 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 11022 |
| UniProt ID | Q9Y2W6 |
| Protein Families | Transmembrane |
| MW | 57 kDa |
| Gene Summary | Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217780L3 | Lenti-ORF clone of TDRKH (Myc-DDK-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4 |
CNY 6,370.00 |
|
| RC217780L4 | Lenti-ORF clone of TDRKH (mGFP-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4 |
CNY 6,370.00 |
|
| RG217780 | TDRKH (tGFP-tagged) - Human tudor and KH domain containing (TDRKH), transcript variant 4 |
CNY 4,940.00 |
|
| SC316232 | TDRKH (untagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4 |
CNY 8,360.00 |
