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DDX58 (NM_014314) Human Tagged ORF Clone
CAT＃: RC217615
- TrueORF®
DDX58 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58)
ORF Plasmid: tGFP
"NM_014314" in other vectors (6)
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CNY 10,160.00
| Cited in 3 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RIG-I; RIG1; RIGI; RLR-1; SGMRT2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217615 representing NM_014314
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCGAGCAGCGACGCAGCCTGCAAGCCTTCCAGGATTATATCCGGAAGACCCTGGACCCTACCT ACATCCTGAGCTACATGGCCCCCTGGTTTAGGGAGGAAGAGGTGCAGTATATTCAGGCTGAGAAAAACAA CAAGGGCCCAATGGAGGCTGCCACACTTTTTCTCAAGTTCCTGTTGGAGCTCCAGGAGGAAGGCTGGTTC CGTGGCTTTTTGGATGCCCTAGACCATGCAGGTTATTCTGGACTTTATGAAGCCATTGAAAGTTGGGATT TCAAAAAAATTGAAAAGTTGGAGGAGTATAGATTACTTTTAAAACGTTTACAACCAGAATTTAAAACCAG AATTATCCCAACCGATATCATTTCTGATCTGTCTGAATGTTTAATTAATCAGGAATGTGAAGAAATTCTA CAGATTTGCTCTACTAAGGGGATGATGGCAGGTGCAGAGAAATTGGTGGAATGCCTTCTCAGATCAGACA AGGAAAACTGGCCCAAAACTTTGAAACTTGCTTTGGAGAAAGAAAGGAACAAGTTCAGTGAACTGTGGAT TGTAGAGAAAGGTATAAAAGATGTTGAAACAGAAGATCTTGAGGATAAGATGGAAACTTCTGACATACAG ATTTTCTACCAAGAAGATCCAGAATGCCAGAATCTTAGTGAGAATTCATGTCCACCTTCAGAAGTGTCTG ATACAAACTTGTACAGCCCATTTAAACCAAGAAATTACCAATTAGAGCTTGCTTTGCCTGCTATGAAAGG AAAAAACACAATAATATGTGCTCCTACAGGTTGTGGAAAAACCTTTGTTTCACTGCTTATATGTGAACAT CATCTTAAAAAATTCCCACAAGGACAAAAGGGGAAAGTTGTCTTTTTTGCGAATCAGATCCCAGTGTATG AACAGCAGAAATCTGTATTCTCAAAATACTTTGAAAGACATGGGTATAGAGTTACAGGCATTTCTGGAGC AACAGCTGAGAATGTCCCAGTGGAACAGATTGTTGAGAACAATGACATCATCATTTTAACTCCACAGATT CTTGTGAACAACCTTAAAAAGGGAACGATTCCATCACTATCCATCTTTACTTTGATGATATTTGATGAAT GCCACAACACTAGTAAACAACACCCGTACAATATGATCATGTTTAATTATCTAGATCAGAAACTTGGAGG ATCTTCAGGCCCACTGCCCCAGGTCATTGGGCTGACTGCCTCGGTTGGTGTTGGGGATGCCAAAAACACA GATGAAGCCTTGGATTATATCTGCAAGCTGTGTGCTTCTCTTGATGCGTCAGTGATAGCAACAGTCAAAC ACAATCTGGAGGAACTGGAGCAAGTTGTTTATAAGCCCCAGAAGTTTTTCAGGAAAGTGGAATCACGGAT TAGCGACAAATTTAAATACATCATAGCTCAGCTGATGAGGGACACAGAGAGTCTGGCAAAGAGAATCTGC AAAGACCTCGAAAACTTATCTCAAATTCAAAATAGGGAATTTGGAACACAGAAATATGAACAATGGATTG TTACAGTTCAGAAAGCATGCATGGTGTTCCAGATGCCAGACAAAGATGAAGAGAGCAGGATTTGTAAAGC CCTGTTTTTATACACTTCACATTTGCGGAAATATAATGATGCCCTCATTATCAGTGAGCATGCACGAATG AAAGATGCTCTGGATTACTTGAAAGACTTCTTCAGCAATGTCCGAGCAGCAGGATTCGATGAGATTGAGC AAGATCTTACTCAGAGATTTGAAGAAAAGCTGCAGGAACTAGAAAGTGTTTCCAGGGATCCCAGCAATGA GAATCCTAAACTTGAAGACCTCTGCTTCATCTTACAAGAAGAGTACCACTTAAACCCAGAGACAATAACA ATTCTCTTTGTGAAAACCAGAGCACTTGTGGACGCTTTAAAAAATTGGATTGAAGGAAATCCTAAACTCA GTTTTCTAAAACCTGGCATATTGACTGGACGTGGCAAAACAAATCAGAACACAGGAATGACCCTCCCGGC ACAGAAGTGTATATTGGATGCATTCAAAGCCAGTGGAGATCACAATATTCTGATTGCCACCTCAGTTGCT GATGAAGGCATTGACATTGCACAGTGCAATCTTGTCATCCTTTATGAGTATGTGGGCAATGTCATCAAAA TGATCCAAACCAGAGGCAGAGGAAGAGCAAGAGGTAGCAAGTGCTTCCTTCTGACTAGTAATGCTGGTGT AATTGAAAAAGAACAAATAAACATGTACAAAGAAAAAATGATGAATGACTCTATTTTACGCCTTCAGACA TGGGACGAAGCAGTATTTAGGGAAAAGATTCTGCACATACAGACTCATGAAAAATTCATCAGAGATAGTC AAGAAAAACCAAAACCTGTACCTGATAAGGAAAATAAAAAACTGCTCTGCAGAAAGTGCAAAGCCTTGGC ATGTTACACAGCTGACGTAAGAGTGATAGAGGAATGCCATTACACTGTGCTTGGAGATGCTTTTAAGGAA TGCTTTGTGAGTAGACCACATCCCAAGCCAAAGCAGTTTTCAAGTTTTGAAAAAAGAGCAAAGATATTCT GTGCCCGACAGAACTGCAGCCATGACTGGGGAATCCATGTGAAGTACAAGACATTTGAGATTCCAGTTAT AAAAATTGAAAGTTTTGTGGTGGAGGATATTGCAACTGGAGTTCAGACACTGTACTCGAAGTGGAAGGAC TTTCATTTTGAGAAGATACCATTTGATCCAGCAGAAATGTCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217615 representing NM_014314
Red=Cloning site Green=Tags(s) MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWF RGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEIL QICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQ IFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEH HLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQI LVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNT DEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRIC KDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARM KDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETIT ILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVA DEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQT WDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKE CFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKD FHFEKIPFDPAEMSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014314 |
| ORF Size | 2775 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014314.4 |
| RefSeq Size | 4372 bp |
| RefSeq ORF | 2778 bp |
| Locus ID | 23586 |
| UniProt ID | O95786 |
| Domains | DEAD, helicase_C |
| Protein Pathways | Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway |
| MW | 106.4 kDa |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2. [provided by RefSeq, Aug 2020] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MiR-139 Induces an Interferon-β Response in Prostate Cancer Cells by Binding to RIG-1
,Nam, RK;Benatar, T;Amemiya, Y;Seth, A;,
Cancer genomics & proteomics
,PubMed ID 33893074
[DDX58]
|
|
A GTPase-activating protein binding protein (G3BP1) / antiviral protein relay conveys arteriosclerotic Wnt signals in aortic smooth muscle cells
,Ramachandran, B;Stabley, JN;Cheng, SL;Behrmann, AS;Gay, A;Li, L;Mead, M;Kozlitina, J;Lemoff, A;Mirzaei, H;Chen, Z;Towler, DA;,
J. Biol. Chem.
,PubMed ID 29626090
[DDX58]
|
|
RIG-I expression in perifascicular myofibers is a reliable biomarker of dermatomyositis
,Suárez-Calvet, X;Gallardo, E;Pinal-Fernandez, I;De Luna, N;Lleixà, C;Díaz-Manera, J;Rojas-García, R;Castellví, I;Martínez, MA;Grau, JM;Selva-O'Callaghan, A;Illa, I;,
Arthritis Res. Ther.
,PubMed ID 28738907
[DDX58]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217615L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), Myc-DDK-tagged |
CNY 12,560.00 |
|
| RC217615L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mGFP tagged |
CNY 9,030.00 |
|
| RC217615L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), Myc-DDK-tagged |
CNY 12,560.00 |
|
| RC217615L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mGFP tagged |
CNY 12,560.00 |
|
| RG217615 | DDX58 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) |
CNY 11,760.00 |
|
| SC115033 | DDX58 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58) |
CNY 10,168.00 |
